| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 702,766 | C→A | 14.6% | intergenic (+14/‑118) | PP_23SD → / → PP_5SD | 23S ribosomal RNA/5S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 702,766 | 0 | C | A | 14.6% | 120.7 / 11.7 | 48 | intergenic (+14/‑118) | PP_23SD/PP_5SD | 23S ribosomal RNA/5S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (20/21); new base A (3/4); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
ACTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAAC > NC_002947/702627‑702910 | aCTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGc < 1:181987/149‑1 (MQ=11) cTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTTGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGct < 2:203969/149‑2 (MQ=2) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCgg < 1:226579/147‑1 (MQ=32) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCgg < 6:237816/147‑1 (MQ=32) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt < 1:68832/147‑3 (MQ=2) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt < 2:49990/147‑3 (MQ=2) gAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCggtggt < 7:46043/144‑1 (MQ=32) ccTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGa > 4:195996/1‑146 (MQ=32) ccTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGa < 3:195996/146‑1 (MQ=32) cTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGa > 7:39787/1‑149 (MQ=32) aaaGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAAGCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGaaa < 5:133115/145‑1 (MQ=17) aaaGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGaaa > 6:133115/1‑145 (MQ=32) gggCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCg > 2:210987/1‑148 (MQ=32) ccGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt < 3:81180/125‑3 (MQ=2) ccGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt > 4:76947/1‑123 (MQ=2) ccGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt > 4:81180/1‑123 (MQ=2) gAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGt < 4:115415/149‑1 (MQ=32) tACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTAGTGCTTTa > 2:21775/1‑149 (MQ=17) cgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACaa < 6:186425/149‑1 (MQ=32) gTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAag < 4:8024/149‑1 (MQ=32) gATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGc > 2:130005/1‑149 (MQ=32) ggatGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAAATCGTGCTTTACACAAGAGCTTc < 4:227053/145‑1 (MQ=17) aGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGAcc > 3:275442/1‑106 (MQ=32) aGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGAcc < 4:275442/106‑1 (MQ=32) gAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAAtt > 7:49705/1‑149 (MQ=32) aGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTc > 6:307533/1‑149 (MQ=32) aCCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCa > 3:270857/1‑149 (MQ=32) aTACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTc < 5:324531/109‑1 (MQ=32) aTACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTc > 6:324531/1‑109 (MQ=32) tACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTg > 1:119142/1‑148 (MQ=32) cTAATTTCCCGTGAGGCTTGACCATATAACACCCAATCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGa < 5:307533/148‑1 (MQ=17) tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACa > 7:212514/1‑149 (MQ=32) tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACa > 7:50571/1‑149 (MQ=32) gCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGaa > 4:102225/1‑149 (MQ=32) gCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGaa < 2:119142/149‑1 (MQ=32) aCCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATTGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTc < 1:130005/148‑1 (MQ=17) aCCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCt > 6:210467/1‑149 (MQ=32) aCCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCt > 2:244401/1‑149 (MQ=32) atatAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTga < 1:210987/149‑1 (MQ=32) aaCACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTgacgac > 7:94192/1‑149 (MQ=32) cacCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCa < 8:212514/149‑1 (MQ=32) ccAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCa > 8:34885/1‑146 (MQ=32) caccgCAATCTGCGTAGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCa > 7:127521/5‑146 (MQ=255) cAATCTGCTACGCAGATTGCGGTTGTGAAGAGGAAAGACCAGAAAATTCGACCTTAACACCATAGCGTCGCAAAACTCGCACATCCGACATCGCTGGGCTGGCCATGTGGCCATTCTGGCAACAGAATTTCTTGACGACCATAGAGCAt < 6:170289/149‑1 (MQ=11) cAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGT‑CATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCAtt < 8:39787/149‑1 (MQ=17) aaTCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCAtt < 8:49705/149‑1 (MQ=32) cTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGa < 8:94192/146‑1 (MQ=31) cTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAac > 3:189226/1‑148 (MQ=31) | ACTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAAC > NC_002947/702627‑702910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |