Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 708,972 A→G 9.1% V458V (GTA→GTG)  murJ → lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947708,9720AG 9.1% 118.6 / 3.0 44V458V (GTA→GTG) murJlipid II flippase
Reads supporting (aligned to +/- strand):  ref base A (20/20);  new base G (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTC  >  NC_002947/708829‑709120
                                                                                                                                                |                                                                                                                                                    
gCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCAGGTTTGCTGTTCTGGAAGCTTCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTActgct                                                                                                                                                 >  1:206899/1‑149 (MQ=255)
         aTCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTGCTGCTGGCGGGCAt                                                                                                                                        >  5:121217/1‑149 (MQ=255)
         aTCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTGCTGCTGGCGGGCAt                                                                                                                                        >  5:126107/1‑149 (MQ=255)
              ccTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGACAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAG‑GCTCATGTCGGCGGTACTGCTGGCGGGCAGGCAc                                                                                                                                    >  6:27798/1‑148 (MQ=255)
                  ggTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTatat                                                                                                                               <  5:27798/149‑1 (MQ=255)
                              aaTGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGTCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTgg                                                                                                                     <  4:13586/147‑1 (MQ=255)
                                   cGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGAAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAgg                                                                                                              <  2:278826/149‑1 (MQ=255)
                                      tttGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGca                                                                                                           >  2:23909/1‑149 (MQ=255)
                                      tttGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGc                                                                                                            >  7:110106/1‑148 (MQ=255)
                                         gCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGTGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGcatc                                                                                                         <  1:256109/148‑1 (MQ=255)
                                          cTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCTCTATATGCCGGCCTGGGAGCAGGGcatcat                                                                                                       <  6:100772/149‑1 (MQ=255)
                                             ttCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTGCTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGc                                                                                                     <  6:126107/148‑1 (MQ=255)
                                             ttCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTGCTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGc                                                                                                     <  6:121217/148‑1 (MQ=255)
                                             ttCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCt                                                                                                    >  7:80623/1‑149 (MQ=255)
                                                   aaGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAgc                                                                                               <  8:110106/148‑1 (MQ=255)
                                                       tGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCt                                                                                                    <  8:30202/139‑1 (MQ=255)
                                                       tGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCt                                                                                                    >  7:30202/1‑139 (MQ=255)
                                                           cAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACta                                                                                                                                  >  1:18080/1‑105 (MQ=255)
                                                           cAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACta                                                                                                                                  <  2:18080/105‑1 (MQ=255)
                                                            aGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGa                                                                                                 >  8:297863/1‑137 (MQ=255)
                                                            aGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGa                                                                                                 <  7:297863/137‑1 (MQ=255)
                                                             gTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCgg                                                                                    <  7:85171/149‑1 (MQ=255)
                                                                          cGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTg                                                                                >  4:133432/1‑140 (MQ=255)
                                                                          cGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTg                                                                                <  3:133432/140‑1 (MQ=255)
                                                                                 cAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGTTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGTGCGCTGATCCTggc                                                                <  2:206899/149‑1 (MQ=255)
                                                                                    ccTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggcggg                                                             >  6:288170/1‑149 (MQ=255)
                                                                                    ccTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGAGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggcggg                                                             >  4:19790/1‑149 (MQ=255)
                                                                                     ctggctggGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggcgggc                                                            <  6:181755/149‑1 (MQ=255)
                                                                                            ggCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGa                                                     <  1:23909/149‑1 (MQ=255)
                                                                                                     ccTGCTCAAGCTTGTGTTGGCGGTGACGGCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggc                                                                >  1:179142/1‑130 (MQ=255)
                                                                                                     ccTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggc                                                                <  2:179142/129‑1 (MQ=255)
                                                                                                       tGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGc                                          >  6:244946/1‑149 (MQ=255)
                                                                                                         cTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggcg                                                               <  4:259100/126‑1 (MQ=255)
                                                                                                         cTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggcg                                                               >  3:259100/1‑126 (MQ=255)
                                                                                                          tCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGc                                       >  1:286567/1‑149 (MQ=255)
                                                                                                              gCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGcgtc                                                         >  6:167130/1‑127 (MQ=255)
                                                                                                              gCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGcgtc                                                         <  5:167130/127‑1 (MQ=255)
                                                                                                                  gtgtTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCt                               >  3:166793/1‑149 (MQ=255)
                                                                                                                              aC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggcgggc                                                            >  3:122658/1‑108 (MQ=255)
                                                                                                                              aC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTggcgggc                                                            <  4:122658/108‑1 (MQ=255)
                                                                                                                              aC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTcc                    <  3:19790/148‑1 (MQ=255)
                                                                                                                                     aTGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCg             >  3:232207/1‑149 (MQ=255)
                                                                                                                                           gcggTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCAttt       <  5:244946/149‑1 (MQ=255)
                                                                                                                                              gTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTtcgc    >  5:271550/1‑149 (MQ=255)
                                                                                                                                                aCTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTtcgctc  >  7:296918/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGCCTCAGCCTGGCTGGGCAGTGTTCCTGCTCAAGCTTGTGTTGGCGGTGAC‑GCTGATGTCGGCGGTACTGCTGGCGGGCATGCACTATATGCCGGCCTGGGAGCAGGGCATCATGCTTGAGCGCTTCCTGCGGCTTGGGGCGCTGATCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTC  >  NC_002947/708829‑709120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑