Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	719,424	C→T	6.6%	A165T (GCA→ACA)	PP_0613 ←	amidase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	719,424	0	C	T	6.6%	180.4 / 2.5	60	A165T (GCA→ACA)	PP_0613	amidase family protein
Reads supporting (aligned to +/- strand):  ref base C (24/32);  new base T (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

CGGTCATGGCGCTGGCCGGCCATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAGCACTGCACCGGCTTCACGCAT  >  NC_002947/719280‑719571                                                                                                                                                   |                                                                                                                                                    cGGTCATGGCGCTGGCCGGCCATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTCCAGCGCGGCGGCTGCCgca                                                                                                                                                 <  2:145879/149‑1 (MQ=255)  ggTCATGGCGCTGGCCGGCCATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGCACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCgcag                                                                                                                                                >  6:318166/1‑149 (MQ=255)        ggCGCTGGCCGGCCATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGcaccac                                                                                                                                          >  4:233465/1‑149 (MQ=255)         gcgcTGGCCGGCCATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGcaccac                                                                                                                                          <  5:232627/148‑1 (MQ=255)               gccggccATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACg                                                                                                                                    <  1:301075/148‑1 (MQ=255)                ccggccATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGaa                                                                                                                                  >  8:230379/1‑149 (MQ=255)                 cggccATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAac                                                                                                                                 <  8:149032/149‑1 (MQ=255)                 cggccATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAac                                                                                                                                 <  8:144938/149‑1 (MQ=255)                  ggccATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAac                                                                                                                                 >  7:174515/1‑148 (MQ=255)                    ccATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAaccac                                                                                                                              <  3:266401/149‑1 (MQ=255)                            gCACATAGCAGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTc                                                                                                                      <  2:237670/149‑1 (MQ=255)                                aTAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGCACCACCGGCAGTCAGcc                                                                                                                  >  8:96935/1‑149 (MQ=255)                                aTAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGcc                                                                                                                  <  1:40435/149‑1 (MQ=255)                                 tAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCt                                                                                                                 <  7:230379/149‑1 (MQ=255)                                   tccGAAGGTCGGTTTGATTCCGAAGGTGCCGGTGAATGCGCAAGGGATGCGAATCGAATCAACGGCATCGGTCTTTTCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGcc                                                                                                                  <  1:256344/147‑1 (MQ=255)                                   gCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTgg                                                                                                               <  3:233465/149‑1 (MQ=255)                                   gCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGAAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTg                                                                                                                <  7:296207/148‑1 (MQ=255)                                     cGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGgtg                                                                                                             >  6:214112/1‑149 (MQ=255)                                       aaGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTc                                                                                                           <  8:174515/149‑1 (MQ=255)                                               tttGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGAAGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGt                                                                                                   >  1:207098/1‑149 (MQ=255)                                                  gATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGc                                                                                                >  5:323812/1‑149 (MQ=255)                                                      ccGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGcc                                                                                                                  <  2:31414/127‑1 (MQ=255)                                                      ccGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGcc                                                                                                                  <  4:249359/127‑1 (MQ=255)                                                      ccGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGcc                                                                                                                  >  3:249359/1‑127 (MQ=255)                                                      ccGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGcc                                                                                                                  >  1:31414/1‑127 (MQ=255)                                                        gAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGTCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGa                                                                                          >  6:76134/1‑149 (MQ=255)                                                        gAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGTCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGa                                                                                          >  6:80642/1‑149 (MQ=255)                                                            gTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGcc                                                                                      <  7:191684/149‑1 (MQ=255)                                                                 ggTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTa                                                                                   >  4:181495/1‑147 (MQ=255)                                                                     aaGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCCGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCgg                                                                             >  2:264692/1‑149 (MQ=255)                                                                      aGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCggg                                                                            <  7:96935/149‑1 (MQ=255)                                                                        gcgcAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCATACGAACCACCGGCAGTCACCCACGAGTCCCACACGTTGCGAGTGATCCCGTACAGCGGGtt                                                                          >  8:283846/1‑149 (MQ=255)                                                                        gcgcAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCggg                                                                            >  1:13001/1‑147 (MQ=255)                                                                        gcgcAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCggg                                                                            <  2:13001/147‑1 (MQ=255)                                                                                        tCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTc                                                          <  6:323812/149‑1 (MQ=255)                                                                                        tCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTc                                                          <  4:108852/149‑1 (MQ=255)                                                                                        tCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTc                                                          >  1:248481/1‑149 (MQ=255)                                                                                             ccaccGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGcc                                                     >  4:272350/1‑149 (MQ=255)                                                                                             ccaccGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGcc                                                     >  2:146456/1‑149 (MQ=255)                                                                                                    cATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGt                                                                    <  4:129142/127‑1 (MQ=255)                                                                                                    cATCG‑‑CTGCCCAGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAActct                                              >  5:4051/1‑149 (MQ=255)                                                                                                              cccTGATGCAGCACCCCCAGGTTCCGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCGCTTTCCAGCCGAACTCTGGCgtggt                                      >  7:258965/1‑149 (MQ=255)                                                                                                              cccTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCgtg                                        <  5:318166/147‑1 (MQ=255)                                                                                                                 tGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCggg                                                                            >  1:282917/1‑108 (MQ=255)                                                                                                                 tGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCggg                                                                            <  2:282917/108‑1 (MQ=255)                                                                                                                    tGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCtt                                <  1:146456/149‑1 (MQ=255)                                                                                                                     gcagcaCCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTg                               >  2:93164/1‑149 (MQ=255)                                                                                                                          aCCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCccac                          <  6:5252/149‑1 (MQ=255)                                                                                                                            ccccAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCccacca                        <  3:181495/149‑1 (MQ=255)                                                                                                                             cccAGGTTCAGCGCGGCGGCTGTCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAg                       <  5:80642/149‑1 (MQ=255)                                                                                                                             cccAGGTTCAGCGCGGCGGCTGTCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAg                       <  5:76134/149‑1 (MQ=255)                                                                                                                             cccAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCGTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCccac                          >  1:170036/1‑146 (MQ=255)                                                                                                                             cccAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCccacca                        <  2:170036/148‑1 (MQ=255)                                                                                                                              ccAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTggtggt                                   <  2:21119/136‑1 (MQ=255)                                                                                                                              ccAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTggtggt                                   >  1:21119/1‑136 (MQ=255)                                                                                                                                 ggTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGGCAAGAccaccacgcca                   <  3:272350/149‑6 (MQ=255)                                                                                                                                         gcggcgGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAGCACTGCACCGGc           <  1:93164/149‑1 (MQ=255)                                                                                                                                           ggcggcTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAGCACTGCACCGGCtt         <  2:207098/149‑1 (MQ=255)                                                                                                                                                cTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAGCACTGCACCGGCTTCACGc    <  2:248481/149‑1 (MQ=255)                                                                                                                                                 tGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAGCACTGCACCGGCTTCACGCa   >  2:278708/1‑149 (MQ=255)                                                                                                                                                   ccGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAGCACTGCACCGGCTTCACGCAt  >  8:162585/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CGGTCATGGCGCTGGCCGGCCATTGCGGCACATAGCCGAAGGTCGGTTTGATGCCGAAGGTGCCGGTGAAGGCGCAAGGGATGCGAATCGAACCACCGGCATCG‑‑CTGCCCTGATGCAGCACCCCCAGGTTCAGCGCGGCGGCTGCCGCAGCACCACCAGACGAACCACCGGCAGTCAGCCTGGTGTCCCACGGGTTGCGAGTGATGCCGTACAGCGGGTTGTCGGTCACCCCTTTCCAGCCGAACTCTGGCGTGGTGGTCTTGCCCACCAGCACTGCACCGGCTTCACGCAT  >  NC_002947/719280‑719571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑