Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 739,976 C→T 8.1% G217G (GGG→GGA)  pilC ← type IV fimbrial assembly protein PilC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947739,9760CT 8.1% 140.7 / 2.4 49G217G (GGG→GGA) pilCtype IV fimbrial assembly protein PilC
Reads supporting (aligned to +/- strand):  ref base C (20/25);  new base T (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

CCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGATCGCCGACACCCCCAGCCCGGTCAGCAACAGCAGCAGCGGGTAGATC  >  NC_002947/739828‑740123
                                                                                                                                                    |                                                                                                                                                   
ccTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGc                                                                                                                                                     >  4:134025/1‑149 (MQ=255)
ccTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGc                                                                                                                                                     >  7:54866/1‑149 (MQ=255)
              ttGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGatatctc                                                                                                                                       >  3:122530/1‑143 (MQ=255)
                  cAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCtgtg                                                                                                                                               >  4:152155/1‑137 (MQ=255)
                  cAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCtgtg                                                                                                                                               <  3:152155/137‑1 (MQ=255)
                   aaaCACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAACTTGATCa                                                                                                                                  >  4:36931/1‑149 (MQ=255)
                      cacGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCAccc                                                                                                                               <  6:78407/149‑1 (MQ=255)
                         gggCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCAtt                                                                                                                            <  3:134025/149‑1 (MQ=255)
                           gCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTgg                                                                                                                          <  2:316677/149‑1 (MQ=255)
                            cAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTggg                                                                                                                         >  7:36923/1‑149 (MQ=255)
                                cccAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGACCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTaaa                                                                                                                     >  6:272743/1‑149 (MQ=255)
                                 ccAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAAt                                                                                                                    >  8:216768/1‑149 (MQ=255)
                                  cAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATg                                                                                                                   >  4:266160/1‑149 (MQ=255)
                                    gCACCCGTCGAGATATCAACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGcc                                                                                                                 >  6:150018/1‑149 (MQ=255)
                                     cACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCg                                                                                                                <  3:36931/149‑1 (MQ=255)
                                     cACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCg                                                                                                                <  4:149785/149‑1 (MQ=255)
                                        ccGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGc                                                                                                              >  6:309649/1‑148 (MQ=255)
                                                atatCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCa                                                                                                     <  4:38485/149‑1 (MQ=255)
                                                 tatCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCAc                                                                                                    <  8:36923/149‑1 (MQ=255)
                                                     cacaGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCAt                                                                                                <  5:309649/149‑1 (MQ=255)
                                                           cgcgcTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCACCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCga                                                                                          <  6:230384/149‑1 (MQ=255)
                                                              gcTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGcc                                                                                             >  7:95303/1‑143 (MQ=255)
                                                              gcTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGcc                                                                                             <  8:95303/143‑1 (MQ=255)
                                                               cTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCTCGAGAAc                                                                                      <  8:298688/149‑1 (MQ=255)
                                                                  gcgcGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGACCATg                                                                                   >  3:141649/1‑149 (MQ=255)
                                                                       tgctgcCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCtctc                                                                              <  4:141649/149‑1 (MQ=255)
                                                                          tgcCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAACTAGATCACCCATTGCGTAAATGCCGGCAACGCACCACCCATCCCCGAGAACAACCtctcca                                                                            >  4:124219/1‑146 (MQ=255)
                                                                           gcCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCtctc                                                                              <  2:191485/145‑1 (MQ=255)
                                                                           gcCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCtctc                                                                              >  1:191485/1‑145 (MQ=255)
                                                                              ggTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGt                                                                                                                        <  1:195114/100‑1 (MQ=255)
                                                                              ggTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGt                                                                                                                        >  2:195114/1‑100 (MQ=255)
                                                                              ggTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTa                                                                       <  5:272820/149‑1 (MQ=255)
                                                                                  aaGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGa                                                                    <  6:240517/148‑1 (MQ=255)
                                                                                          gcACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAAt                                                                          >  6:177906/1‑134 (MQ=255)
                                                                                          gcACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAAt                                                                          <  5:177906/134‑1 (MQ=255)
                                                                                          gcACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGACAACCGGGTCAGCGCGGTGGACAAGTTGATCACCCATTGGGGAAATGCCGGCAACGCCGCCCGCATGCCCGAGCACATGATCTCGAATTTAGGAATCAGCTcc                                                           >  2:150087/1‑149 (MQ=255)
                                                                                                 aaCACCCATCACCACGACCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGcaaca                                                    <  5:272743/149‑1 (MQ=255)
                                                                                                      ccATCACCTCGGCCATCACCAGCAGCAGCGGCGCAAACCGGCTCTGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATTCCGGCAACGCCGCAGCCATGCCCGAGAACATGCTCTAGAATTTa                                                                       <  7:135661/125‑1 (MQ=255)
                                                                                                        aTCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGa                                                                    <  7:294679/126‑1 (MQ=255)
                                                                                                        aTCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGa                                                                    >  8:294679/1‑126 (MQ=255)
                                                                                                           accacGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGATCGCCGa                                          <  3:266160/149‑1 (MQ=255)
                                                                                                                        ccAGCAGCAGCGGCGCAAACCGGCTCAGTCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATg                                                                                               <  4:125973/83‑1 (MQ=255)
                                                                                                                        ccAGCAGCAGCGGCGCAAACCGGCTCAGTCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATg                                                                                               <  4:105910/83‑1 (MQ=255)
                                                                                                                        ccAGCAGCAGCGGCGCAAACCGGCTCAGTCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATg                                                                                               >  3:125973/1‑83 (MQ=255)
                                                                                                                        ccAGCAGCAGCGGCGCAAACCGGCTCAGTCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATg                                                                                               >  3:105910/1‑83 (MQ=255)
                                                                                                                            cagcagcGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGATCGCCGACACCCCCAGCCCGGTCa                         <  7:216768/149‑1 (MQ=255)
                                                                                                                               cagcGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGATCGCCGACACCCCCAGCCCGGTCAGCa                      <  5:150018/149‑1 (MQ=255)
                                                                                                                                       cAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAGCATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGCTCGCCGACACCCCCAGCCCGGTCAGCAAccgccgc              >  8:271433/1‑149 (MQ=255)
                                                                                                                                                tCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGATCGCCGACACCCCCAGCCCGGTCAGCAACAGCAGCAGCggg        >  7:118753/1‑146 (MQ=255)
                                                                                                                                                tCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGATCGCCGACACCCCCAGCCCGGTCAGCAACAGCAGCAGCggg        >  2:248641/1‑146 (MQ=255)
                                                                                                                                                   gCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGATCGCCGACACCCCCAGCCCGGTCAGCAACAGCAGCAGCGGGTAGATc  <  8:118753/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCACCCATTGGGTAAATGCCGGCAACGCCGCACCCATGCCCGAGAACATGCTCTCGAATTTAGGAATCACCTCCAGCAACAAGATCGCCGACACCCCCAGCCCGGTCAGCAACAGCAGCAGCGGGTAGATC  >  NC_002947/739828‑740123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑