Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	750,543	T→C	5.5%	R107R (AGA→AGG)	PP_0640 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	750,543	0	T	C	5.5%	221.1 / 2.4	73	R107R (AGA→AGG)	PP_0640	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (35/34);  new base C (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

ACATGTGCTCAAAGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAACCTTCCATTTGCCACTT  >  NC_002947/750413‑750678                                                                                                                                   |                                                                                                                                        aCATGTGCTCAAAGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGa                                                                                                                       >  1:34656/1‑149 (MQ=255)     gtgCTCAAAGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACATAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAg                                                                                                                   >  3:31457/1‑149 (MQ=255)         tCAAAGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGc                                                                                                                          >  1:210379/1‑138 (MQ=255)         tCAAAGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGc                                                                                                                          <  2:210379/138‑1 (MQ=255)         tCAAAGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGAt                                                                                                               >  6:287596/1‑149 (MQ=255)         tCAAAGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTATGCCTGAGTAATATCTCGCCATAACCAGCCGACCAGCGAt                                                                                                               >  2:116954/1‑149 (MQ=255)           aaaGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGAc                                                                                                                      <  5:88877/140‑1 (MQ=255)           aaaGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGAc                                                                                                                      >  6:88877/1‑140 (MQ=255)           aaaGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGAt                                                                                                               <  2:34656/147‑1 (MQ=255)           aaaGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATgg                                                                                                             <  3:181437/149‑1 (MQ=255)           aaaGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATg                                                                                                              >  4:239979/1‑148 (MQ=255)                   ggTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGTTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCGTCTCTCGCCATAACCAGCCGACCa                                                                                                                    <  1:200028/134‑1 (MQ=255)                   ggTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCGTCTCTCGCCATAACCAGCCGACCa                                                                                                                    >  2:200028/1‑134 (MQ=255)                    gTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCt                                                                                                    <  6:110297/149‑1 (MQ=255)                     tGCTTGATCTCATCGCGTCGTACAACTACAATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTc                                                                                                   <  5:302343/149‑1 (MQ=255)                      gCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCATGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCt                                                                                                    >  6:248485/1‑147 (MQ=255)                       cTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCa                                                                                                  <  5:248485/148‑1 (MQ=255)                       cTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCa                                                                                                  <  7:90887/148‑1 (MQ=255)                               cATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAAct                                                                                         <  3:10090/149‑1 (MQ=255)                                aTCGCGTCTTACAACTACCATAATTTGGTCGCTAGGGACTTTATAGCTAACTCTGTACGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAAct                                                                                         <  6:149020/148‑1 (MQ=255)                                      tcgtACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCg                                                                                                                                     <  2:225527/98‑1 (MQ=255)                                      tcgtACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCg                                                                                                                                     >  1:225527/1‑98 (MQ=255)                                            aaCTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGa                                                                              >  2:251476/1‑147 (MQ=255)                                            aaCTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGa                                                                              >  2:51171/1‑147 (MQ=255)                                            aaCTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGa                                                                              >  6:23840/1‑147 (MQ=255)                                              cTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGc                                                                          >  5:113481/1‑149 (MQ=255)                                              cTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGc                                                                          >  8:168297/1‑149 (MQ=255)                                               tACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTAc                                                                                     >  6:110177/1‑137 (MQ=255)                                               tACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTAc                                                                                     <  5:110177/137‑1 (MQ=255)                                                aCCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCt                                                                         >  8:71212/1‑148 (MQ=255)                                                 ccATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGcc                                                                                                                         >  2:77622/1‑99 (MQ=255)                                                 ccATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGcc                                                                                                                         <  1:77622/99‑1 (MQ=255)                                                 ccATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTc                                                                        >  6:246044/1‑148 (MQ=255)                                                       gTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCa                                                                                                  <  2:277181/116‑1 (MQ=255)                                                       gTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCa                                                                                                  >  1:277181/1‑116 (MQ=255)                                                                tAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGAt                                                                  <  2:312945/139‑1 (MQ=255)                                                                tAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGAt                                                                  >  1:312945/1‑139 (MQ=255)                                                                tAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACACTACACCCAGAAAGCTCAAGGATGTAAAgctgc                                                        >  4:85989/1‑149 (MQ=255)                                                                    gACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCtt                                                    <  1:51171/149‑1 (MQ=255)                                                                       tttATAGCTAACTCTGTCCGACCCCTTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACACACAGAAAGATCAAGGATGTAAAgctg                                                         >  4:22256/1‑141 (MQ=255)                                                                       tttATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGa                                                 <  7:71212/149‑1 (MQ=255)                                                                       tttATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCCCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTc                                                   <  8:101184/147‑1 (MQ=255)                                                                       tttATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCCCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTc                                                   >  7:101184/1‑147 (MQ=255)                                                                       tttATAGCTAACTCTGTCCGACCACGTTTTCTTTAAAACAAAACCTTTGACTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAgctg                                                         <  3:22256/141‑1 (MQ=255)                                                                                       tCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAACGCTGCTCTTCGATCATTTGGCCTTTTGt                                 <  5:246044/149‑1 (MQ=255)                                                                                       tCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCtttt                                   <  1:116954/147‑1 (MQ=255)                                                                                       tCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCtttt                                   <  8:172374/147‑1 (MQ=255)                                                                                       tCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTga                                 >  7:172374/1‑148 (MQ=255)                                                                                         cGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGtt                                <  7:168297/148‑1 (MQ=255)                                                                                          gACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCt                              >  1:181476/1‑149 (MQ=255)                                                                                           aCCCCTTTTTCATTAAAACAACACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGCCCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCtt                             <  4:31457/149‑1 (MQ=255)                                                                                           aCCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCtt                             <  2:181476/149‑1 (MQ=255)                                                                                             cccGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTc                           <  5:287596/149‑1 (MQ=255)                                                                                                gTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCaaa                        <  3:239979/149‑1 (MQ=255)                                                                                                     cTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATaa                   >  8:15751/1‑149 (MQ=255)                                                                                                     cTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATaa                   >  4:223591/1‑149 (MQ=255)                                                                                                      tttAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAatat                     >  4:169075/1‑146 (MQ=255)                                                                                                      tttAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAatat                     <  3:169075/146‑1 (MQ=255)                                                                                                      tttAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAAc                  >  6:167599/1‑149 (MQ=255)                                                                                                      tttAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAAc                  >  5:204090/1‑149 (MQ=255)                                                                                                      tttAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAAc                  <  5:276304/149‑1 (MQ=255)                                                                                                      tttAAAACAAAACCTTTGCCTGAGTCATCCCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGAt                                                <  6:51201/119‑1 (MQ=255)                                                                                                      tttAAAACAAAACCTTTGCCTGAGTCATCCCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGAt                                                >  5:51201/1‑119 (MQ=255)                                                                                                       ttAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAAcc                 >  3:152270/1‑149 (MQ=255)                                                                                                        tAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAAcc                 <  5:167599/148‑1 (MQ=255)                                                                                                            acaaaacCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAACCTTCCa            <  7:15751/149‑1 (MQ=255)                                                                                                                   cTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAACCTTc              <  2:215219/140‑1 (MQ=255)                                                                                                                   cTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAACCTTc              >  1:215219/1‑140 (MQ=255)                                                                                                                   cTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAACCTTCCATTTGCCa     >  5:188788/1‑149 (MQ=255)                                                                                                                      tGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAACCTTCCATTTGCCAc    <  5:23840/147‑1 (MQ=255)                                                                                                                        ccTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGt                                 >  7:106875/1‑116 (MQ=255)                                                                                                                        ccTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGt                                 <  8:106875/116‑1 (MQ=255)                                                                                                                        ccTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAACCTTCCATTTGCCACtt  >  8:14941/1‑147 (MQ=255)                                                                                                                                  ctctcGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCtt                                                    <  3:149780/87‑1 (MQ=255)                                                                                                                                  ctctcGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCtt                                                    >  4:149780/1‑87 (MQ=255)                                                                                                                                   |                                                                                                                                        ACATGTGCTCAAAGAATGGGTGCTGGATCTCATCGCGTCGTACAACTACCATAAGTTGGTCGCTAGGGACTTTATAGCTAACTCTGTCCGACCCCGTTTTCTTTAAAACAAAACCTTTGCCTGAGTCATCTCTCGCCATAACCAGCCGACCAGCGATGGACATCGCCTCAGATACAACTCTACCCCCAGAAAGCTCAAGGATGTAAAGCTGCTCTTCGATCATTTGGCCTTTTGTTCTTTCAAATATAACCTTCCATTTGCCACTT  >  NC_002947/750413‑750678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑