Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	762,579	A→T	6.3%	E204V (GAA→GTA)	mdh →	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	762,579	0	A	T	6.3%	191.3 / 2.5	63	E204V (GAA→GTA)	mdh	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (23/36);  new base T (2/2);  total (25/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CTTCATCGCCGAGCAAACCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTATTTTGCCGGCGGTGGC  >  NC_002947/762433‑762726                                                                                                                                                   |                                                                                                                                                    cTTCATCGCCGAGCAAACCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGTGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGaa                                                                                                                                                    <  2:32182/148‑1 (MQ=255)   tCATCGCCGAGCAAACCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGaa                                                                                                                                                    >  5:257379/1‑146 (MQ=255)     aTCGCCGAGCAAACCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGa                                                                                                                                                <  7:254164/148‑1 (MQ=255)     aTCGCCGAGCAAACAGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGAt                                                                                                                                               <  5:819/149‑1 (MQ=255)      tCGCCGAGCAAACCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGAtt                                                                                                                                              <  8:274114/149‑1 (MQ=255)      tCGCCGAGCAAACCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGAtt                                                                                                                                              <  8:170961/149‑1 (MQ=255)         ccGAGCAAACCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTc                                                                                                                                           >  4:157085/1‑149 (MQ=255)                 aCCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCAc                                                                                                                                   <  5:44339/149‑1 (MQ=255)                 aCCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCAc                                                                                                                                   <  1:40444/149‑1 (MQ=255)                          tCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAggg                                                                                                                          >  2:117314/1‑149 (MQ=255)                           cTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAggg                                                                                                                          <  1:48151/148‑1 (MQ=255)                             gCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAgg                                                                                                                           >  5:135149/1‑145 (MQ=255)                             gCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAgg                                                                                                                           <  6:135149/145‑1 (MQ=255)                              cACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGggcggc                                                                                                                      >  8:197845/1‑149 (MQ=255)                              cACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGggcggc                                                                                                                      >  8:28479/1‑149 (MQ=255)                              cACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGCGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTc                                                                                                                                           <  5:214371/128‑1 (MQ=255)                              cACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGAGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTc                                                                                                                                           >  6:214371/1‑128 (MQ=255)                               aCGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGa                                                                                                                                                     <  3:38389/117‑1 (MQ=255)                               aCGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGa                                                                                                                                                     >  4:38389/1‑117 (MQ=255)                                cGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGggcggcgg                                                                                                                    <  1:117314/149‑1 (MQ=255)                                         aCGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCt                                                                                                           >  7:294036/1‑149 (MQ=255)                                         aCGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCt                                                                                                           >  8:10493/1‑149 (MQ=255)                                          cGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTg                                                                                                          <  3:157085/149‑1 (MQ=255)                                          cGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTg                                                                                                          <  6:296538/149‑1 (MQ=255)                                           ggCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTgg                                                                                                         <  7:143549/149‑1 (MQ=255)                                            gCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTggg                                                                                                        <  6:257379/149‑1 (MQ=255)                                            gCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTggg                                                                                                        <  4:294652/149‑1 (MQ=255)                                             cGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGAGGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTgggg                                                                                                       <  1:36889/149‑1 (MQ=255)                                               ttGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGtt                                                                                                     >  4:243193/1‑149 (MQ=255)                                                  gTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTAGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGGAAGGGCGGCGGCGAGATTCTGGGGTTg                                                                                                    >  7:28045/1‑147 (MQ=255)                                                         gcgggcATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACg                                                                                           <  3:180015/149‑1 (MQ=255)                                                         gcgggcATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACg                                                                                           <  8:294036/149‑1 (MQ=255)                                                          cgggcATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACgg                                                                                          <  7:28479/149‑1 (MQ=255)                                                               aTGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTgaaga                                                                                                >  2:234584/1‑138 (MQ=255)                                                               aTGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTgaaga                                                                                                <  1:234584/138‑1 (MQ=255)                                                                 ggCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAgcgc                                                                                   >  1:179023/1‑149 (MQ=255)                                                                     aCAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGc                                                                               <  2:179023/149‑1 (MQ=255)                                                                       aGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGa                                                                             <  3:265046/149‑1 (MQ=255)                                                                         cATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATg                                                                           >  5:26134/1‑149 (MQ=255)                                                                                      aTGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGc                                                              <  6:26134/149‑1 (MQ=255)                                                                                               tGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTgg                                                                                                         <  6:38726/97‑1 (MQ=255)                                                                                               tGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTgg                                                                                                         >  5:38726/1‑97 (MQ=255)                                                                                                 ccAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGt                                                                 <  4:308311/135‑1 (MQ=255)                                                                                                 ccAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGt                                                                 >  3:308311/1‑135 (MQ=255)                                                                                                   aGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATg                                                 >  2:194294/1‑149 (MQ=255)                                                                                                    gATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCTAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGAt                                                                            <  7:7347/121‑1 (MQ=255)                                                                                                    gATTGGCTCGGTGCCGTTGTCGCACTTACTGTCCAGCGAGCAGATCGAACAGATTCTCGAGCGCACCCGTAAGGGCGCCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTCCGAt                                                                            >  8:7347/1‑121 (MQ=255)                                                                                                     aTTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGt                                               >  6:319115/1‑149 (MQ=255)                                                                                                      ttGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTc                                              <  3:243193/149‑1 (MQ=255)                                                                                                         gCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGAt                                           <  5:319115/149‑1 (MQ=255)                                                                                                          cTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGTAGCGGCTGCGATGCACCGGGCGTAGCCATTGCGCAGATGGTCGATg                                          <  7:197845/149‑1 (MQ=255)                                                                                                              gTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCgaga                                                                                                               <  6:31248/76‑1 (MQ=255)                                                                                                              gTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCgaga                                                                                                               >  5:31248/1‑76 (MQ=255)                                                                                                              ccgatgTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACGGGGCGTGTCCATTGCGCAGATGGTCGATGCGa                                       <  5:99258/143‑1 (MQ=255)                                                                                                                      gTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCCATGCGa                                       >  6:99258/1‑140 (MQ=255)                                                                                                                                 tGTCCAGCGAGCAGATCGTACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCg                    >  5:298145/1‑148 (MQ=255)                                                                                                                                 tGTCCAGCGAGCAGATCGTACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCg                    >  5:293496/1‑148 (MQ=255)                                                                                                                                 tGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCg                    >  3:92262/1‑148 (MQ=255)                                                                                                                                 tGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCg                    >  1:226931/1‑148 (MQ=255)                                                                                                                                   tCCAGCGAGCAGATCGTACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGt                   <  6:293496/147‑1 (MQ=255)                                                                                                                                   tCCAGCGAGCAGATCGTACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGt                   <  6:298145/147‑1 (MQ=255)                                                                                                                                   tCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTAt                 <  6:299628/149‑1 (MQ=255)                                                                                                                                       gCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTATTTTg             >  6:240652/1‑149 (MQ=255)                                                                                                                                       gCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTATTTTg             <  1:194294/149‑1 (MQ=255)                                                                                                                                            cAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTATTTTGCcggc        <  4:92262/149‑1 (MQ=255)                                                                                                                                             aGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTATTTTGCcggcg       <  7:10493/149‑1 (MQ=255)                                                                                                                                                  gAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTATTTTGCCGGCGGTgg   <  5:240652/148‑1 (MQ=255)                                                                                                                                                   aaCAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTATTTTGCCGGCGGTGGc  >  7:191969/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CTTCATCGCCGAGCAAACCGGGTTTTCTGCACGGGATATCACGGCGTTGGTGCTGGGCGGGCATGGCGACAGCATGGTGCCGCTGATGCGCTACTGCCAGATTGGCTCGGTGCCGTTGTCGCACTTCCTGTCCAGCGAGCAGATCGAACAGATTGTCGAGCGCACCCGTAAGGGCGGCGGCGAGATTCTGGGGTTGAAGAAGACGGGGAGCGCCTGCGATGCACCGGGCGTGGCCATTGCGCAGATGGTCGATGCGATCGCCAATGGGCGGAACCGTATTTTGCCGGCGGTGGC  >  NC_002947/762433‑762726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑