Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 765,351 T→A 8.1% intergenic (‑7/‑55) PP_0657 ← / → PP_0658 amino acid ABC transporter substrate‑binding protein/homocysteine S‑methyltransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947765,3510TA 8.1% 173.4 / 4.0 61intergenic (‑7/‑55)PP_0657/PP_0658amino acid ABC transporter substrate‑binding protein/homocysteine S‑methyltransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (25/31);  new base A (2/3);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTAGCTTTCCATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTAATTCTGGACGGTGGCATGGGCCGAGAGCTGCAGCG  >  NC_002947/765208‑765497
                                                                                                                                               |                                                                                                                                                  
gggTAGCTTTCCATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGtt                                                                                                                                               >  8:124805/1‑149 (MQ=255)
 ggTAGCTTTCCATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTg                                                                                                                                              <  2:250344/149‑1 (MQ=255)
       tttCCATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCaaa                                                                                                                                        >  4:126438/1‑149 (MQ=255)
          ccATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGAAATGTTgg                                                                                                                                             >  6:23599/1‑141 (MQ=255)
          ccATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGAAATGTTgg                                                                                                                                             <  5:19008/141‑1 (MQ=255)
          ccATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGAAATGTTgg                                                                                                                                             <  5:23599/141‑1 (MQ=255)
          ccATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGAAATGTTgg                                                                                                                                             >  6:19008/1‑141 (MQ=255)
            aTCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGt                                                                                                                                   >  5:191636/1‑149 (MQ=255)
               aaCACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTgg                                                                                                                                             <  3:279433/136‑1 (MQ=255)
               aaCACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTgg                                                                                                                                             >  4:279433/1‑136 (MQ=255)
                  acGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAgg                                                                                                                             >  1:181523/1‑149 (MQ=255)
                  acGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAgg                                                                                                                             <  2:181523/149‑1 (MQ=255)
                            tttCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTaa                                                                                                                     >  1:47499/1‑147 (MQ=255)
                            tttCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATa                                                                                                                   <  7:5392/149‑1 (MQ=255)
                            tttCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATa                                                                                                                   <  7:150785/149‑1 (MQ=255)
                            tttCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATa                                                                                                                   <  7:136948/149‑1 (MQ=255)
                            tttCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATa                                                                                                                   <  4:248747/149‑1 (MQ=255)
                              tCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAg                                                                                                                  <  3:299623/148‑1 (MQ=255)
                                cTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGtt                                                                                                                <  2:55374/148‑1 (MQ=255)
                                    cgcgATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAAc                                                                                                           <  4:148960/149‑1 (MQ=255)
                                        aTTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCt                                                                                                        >  3:274561/1‑148 (MQ=255)
                                            aaCCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATcc                                                                                                   <  6:191636/149‑1 (MQ=255)
                                              cccGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGATGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATccc                                                                                                  >  8:167145/1‑148 (MQ=255)
                                                 gCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAAt                                                                                                                                       <  2:294045/108‑1 (MQ=255)
                                                 gCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAAt                                                                                                                                       >  1:294045/1‑108 (MQ=255)
                                                  cTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATaa                                                                                             <  3:129350/149‑1 (MQ=255)
                                                  cTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGATGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATaa                                                                                             <  7:167145/149‑1 (MQ=255)
                                                   tCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCTAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATaa                                                                                             >  7:132075/1‑148 (MQ=255)
                                                     cAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTa                                                                                                       >  6:45994/1‑136 (MQ=255)
                                                     cAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTa                                                                                                       <  5:45994/136‑1 (MQ=255)
                                                     cAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATaa                                                                                             <  8:132075/146‑1 (MQ=255)
                                                         gTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAAttt                                                                                      >  2:171204/1‑149 (MQ=255)
                                                            gcgcCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGATAAGAAAATCGCATGTACATACCTGAAATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTa                                                                                    <  3:14106/148‑1 (MQ=255)
                                                                cAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCAt                                                                               <  4:274561/149‑1 (MQ=255)
                                                                   cAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCAt                                                                               >  4:178683/1‑146 (MQ=255)
                                                                   cAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCAt                                                                               >  8:290532/1‑146 (MQ=255)
                                                                   cAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTAATCAt                                                                               >  4:75528/1‑146 (MQ=255)
                                                                         cTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCtt                                                                      <  1:171204/149‑1 (MQ=255)
                                                                         cTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCtt                                                                      <  6:239014/149‑1 (MQ=255)
                                                                            gggTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCa                                                                    <  7:276702/148‑1 (MQ=255)
                                                                              gTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAAttt                                                                                      <  5:86262/128‑1 (MQ=255)
                                                                              gTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAAttt                                                                                      >  6:86262/1‑128 (MQ=255)
                                                                              gTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCa                                                                    >  3:98911/1‑146 (MQ=255)
                                                                                 gCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGaa                                                               <  4:98911/148‑1 (MQ=255)
                                                                                     ttGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCag                                                                   <  4:225989/140‑1 (MQ=255)
                                                                                           gCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTATTCATAAGAACCTTCAGAGAAGTTATATg                                                       <  1:17998/146‑1 (MQ=255)
                                                                                           gCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATg                                                       >  2:17998/1‑146 (MQ=255)
                                                                                                aCAAGGCCCGAACCCAGCAGAGAAGAAAATCTCATGTACATAACTGATATGTTGGGCAAATGGGTTATATTGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGc                                                <  3:75528/148‑1 (MQ=255)
                                                                                                 cAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCg                                               <  7:290532/148‑1 (MQ=255)
                                                                                                     gCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCaga                                                                  <  6:171364/125‑1 (MQ=255)
                                                                                                     gCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCaga                                                                  >  5:171364/1‑125 (MQ=255)
                                                                                                           aCCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTaa                                    >  8:235402/1‑149 (MQ=255)
                                                                                                                    agaagaAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTAGTTAATAGTTATAACCCTAATCCCCATATTGTTTTTTACTCATAAGAACGTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTAAATCTGGagg                           >  4:38551/1‑147 (MQ=255)
                                                                                                                       agaaAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTAATTCTGGACGGt                         <  7:124805/148‑1 (MQ=255)
                                                                                                                                    tACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTAATTCTGGACGGTGGCATGGGCCgag            <  1:214089/148‑1 (MQ=255)
                                                                                                                                     aCATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTAATTCTGGACGGTGGCATGGGCCgaga           <  3:178683/148‑1 (MQ=255)
                                                                                                                                         aCCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATa                                                                                                                   >  6:40384/1‑40 (MQ=255)
                                                                                                                                         aCCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATa                                                                                                                   <  5:40384/40‑1 (MQ=255)
                                                                                                                                           cTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTAATTCTGGACGGTGGCATGGGCCGAGAGCTgcag    >  1:253516/1‑149 (MQ=255)
                                                                                                                                             gATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATACCCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTAATTCTGGACGGTGGCATGGGCCGAGAGCTgcagcg  >  3:30734/1‑149 (MQ=255)
                                                                                                                                             gATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATg                                        >  4:62430/1‑111 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GGGTAGCTTTCCATCAACACGTTGACCATTTCCTTGCGCGATTCAACCCGCTCCAACGTGGCGCCAGCAAAGGCTTGGGTGGCAGTTGCGAGCAGTACAAGGCCCGCACCCAGCAGAGAAGAAAATCGCATGTACATACCTGATATGTTGGGCAAATGGGTGATAGGGTATTTAATAGTTATAACCCTAATCCCCATAATGAATTTTACTCATAAGAACCTTCAGAGAAGTTATATGGCAGAGCGAACGATGGTAATTCTGGACGGTGGCATGGGCCGAGAGCTGCAGCG  >  NC_002947/765208‑765497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑