Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 15,617 G→A 7.5% R763H (CGT→CAT)  gyrB → DNA gyrase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294715,6170GA 7.5% 152.8 / 2.9 53R763H (CGT→CAT) gyrBDNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

GCCGCAAGGCAATCGTCGAGTTCAAAGAGGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGT  >  NC_002947/15470‑15756
                                                                                                                                                   |                                                                                                                                           
gccgcAAGGCAATCGTCGAGTTCAAAGAGGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCg                                                                                                                                             >  7:271156/1‑148 (MQ=255)
       ggCAATCGTCGAGTTCAAAGAGGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTc                                                                                                                                      >  6:98737/1‑148 (MQ=255)
         cAATCGTCGAGTTCAAAGAGGGCCGGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAg                                                                                                                                   <  4:64419/149‑1 (MQ=255)
                cgAGTTCAAAGAGGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGa                                                                                                                            >  1:136105/1‑149 (MQ=255)
                     tCAAAGAGGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGa                                                                                                                        <  3:184748/148‑1 (MQ=255)
                       aaaGAGGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGa                                                                                                                     <  2:136105/149‑1 (MQ=255)
                         agagGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATATGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCaa                                                                                                                                    >  3:291142/1‑132 (MQ=255)
                         agagGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATATGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCaa                                                                                                                                    <  4:291142/132‑1 (MQ=255)
                              gCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATc                                                                                                              <  5:98737/149‑1 (MQ=255)
                               ccTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGAt                                                                                                                           >  8:163038/1‑135 (MQ=255)
                               ccTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGAt                                                                                                                           <  7:163038/135‑1 (MQ=255)
                               ccTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATcg                                                                                                             >  5:211017/1‑149 (MQ=255)
                                   gATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCTAGGTCACGATCGAAGATGCTATcgccgc                                                                                                         <  1:179860/149‑1 (MQ=255)
                                        gCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGAc                                                                                                     >  6:326423/1‑148 (MQ=255)
                                                  gagaCCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCaa                                                                                          <  8:215359/149‑1 (MQ=255)
                                                   agaCCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAac                                                                                         <  5:326423/149‑1 (MQ=255)
                                                        accaAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCt                                                                                    >  3:209402/1‑149 (MQ=255)
                                                            aGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATg                                                                                <  5:115178/149‑1 (MQ=255)
                                                              cgcCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATggg                                                                              <  8:271156/149‑1 (MQ=255)
                                                                 cATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGa                                                                           >  2:20683/1‑149 (MQ=255)
                                                                       aTTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGAcc                                                                                                    >  5:122509/1‑118 (MQ=255)
                                                                       aTTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGAcc                                                                                                    <  6:122509/118‑1 (MQ=255)
                                                                           aGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGa                                                                  <  4:209402/148‑1 (MQ=255)
                                                                            gCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGa                                                                  <  6:61144/147‑1 (MQ=255)
                                                                            gCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGa                                                                  >  5:61144/1‑147 (MQ=255)
                                                                            gCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGc                                                                >  8:261076/1‑149 (MQ=255)
                                                                             cGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGcc                                                               >  6:294208/1‑149 (MQ=255)
                                                                                tACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGAcgcg                                                            >  7:199806/1‑149 (MQ=255)
                                                                                   aaaGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGcgtcg                                                         >  5:286609/1‑149 (MQ=255)
                                                                                       ggCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCgg                                                                      <  2:113057/132‑1 (MQ=255)
                                                                                        gCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACt                                                    >  4:205028/1‑149 (MQ=255)
                                                                                                 gaTGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGaaa                                           <  6:211017/149‑1 (MQ=255)
                                                                                                  aTGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAg                                          <  3:205028/149‑1 (MQ=255)
                                                                                                      aCCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAAc                                      >  5:252597/1‑149 (MQ=255)
                                                                                                        ccGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAAcgc                                    <  7:261076/149‑1 (MQ=255)
                                                                                                        ccGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAAcgc                                    >  8:12660/1‑149 (MQ=255)
                                                                                                               aaCTGTGGGAAACCACGATGGAGCCGACCGTTGGGCGTATGCTCAAGGTCTCGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTc                                                                    <  6:312429/110‑1 (MQ=255)
                                                                                                               aaCTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGAACCGGTc                                                                    >  5:312429/1‑110 (MQ=255)
                                                                                                                aCTGTGGGAAACCACCATGGACCCGACCGTGCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGCTGGGTGATGCGGTCGAGCCGCGTCGTGCCTTCATCGAAAGTAACGCGCTGTCgg                            >  2:150414/1‑149 (MQ=255)
                                                                                                                        aaaCCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGcc                                                               <  6:314097/106‑1 (MQ=255)
                                                                                                                        aaaCCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGcc                                                               >  5:314097/1‑106 (MQ=255)
                                                                                                                            caccaTGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTg                 >  5:50259/1‑148 (MQ=255)
                                                                                                                              ccaTGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGAc              >  4:109020/1‑149 (MQ=255)
                                                                                                                              ccaTGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGAc              <  5:294208/149‑1 (MQ=255)
                                                                                                                                 tGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTc           <  6:252597/149‑1 (MQ=255)
                                                                                                                                      ccGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAg                                                                 >  4:284639/1‑90 (MQ=255)
                                                                                                                                      ccGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAg                                                                 <  3:284639/90‑1 (MQ=255)
                                                                                                                                      ccGACCGTTCGCCATATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAAcg                                     <  7:212064/118‑1 (MQ=255)
                                                                                                                                      ccGACCGTTCGCCATATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAAcg                                     <  7:207863/118‑1 (MQ=255)
                                                                                                                                      ccGACCGTTCGCCATATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAAcg                                     >  8:207863/1‑118 (MQ=255)
                                                                                                                                      ccGACCGTTCGCCATATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAAcg                                     >  8:212064/1‑118 (MQ=255)
                                                                                                                                          ccGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGgt  <  6:286609/149‑1 (MQ=255)
                                                                                                                                             ttCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTg         <  3:204948/139‑1 (MQ=255)
                                                                                                                                             ttCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTg         >  4:204948/1‑139 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GCCGCAAGGCAATCGTCGAGTTCAAAGAGGGCCTGGATTGGCTGATGAACGAGACCACCAAGCGCCATACGATTCAGCGATACAAAGGGCTGGGTGAGATGAACCCGGATCAACTGTGGGAAACCACCATGGACCCGACCGTTCGCCGTATGCTCAAGGTCACGATCGAAGATGCTATCGCCGCTGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAGCCGCGTCGTGACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGT  >  NC_002947/15470‑15756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑