Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 799,847 A→T 8.3% L209Q (CTG→CAG)  ispB ← octaprenyl diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947799,8470AT 8.3% 134.0 / 2.5 48L209Q (CTG→CAG) ispBoctaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base T (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTC  >  NC_002947/799705‑799995
                                                                                                                                              |                                                                                                                                                    
gCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCTGGTCGACGACctgctg                                                                                                                                                  <  1:247462/147‑1 (MQ=255)
 cGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGaa                                                                                                                                               <  1:155568/149‑1 (MQ=255)
                tGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATg                                                                                                                                >  5:101166/1‑149 (MQ=255)
                     ggCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCg                                                                                                                            <  2:57700/148‑1 (MQ=255)
                         gTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCaaa                                                                                                                       <  4:7846/149‑1 (MQ=255)
                            ccTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGt                                                                                                                    <  8:116184/149‑1 (MQ=255)
                              ttCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGaa                                                                                                                                               >  5:8493/1‑120 (MQ=255)
                              ttCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGaa                                                                                                                                               <  6:8493/120‑1 (MQ=255)
                                        gtgtTGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGGCACCGACGTTCTTGGTCTGGGGCTGGGTGTTACCCTTGTCGTCCAGCAGGGCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTc                                                                                                        <  1:257388/149‑1 (MQ=255)
                                        gtgtAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGGCGTCACCGACGTGCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCtt                                                                                                         <  6:190881/148‑1 (MQ=255)
                                                      cAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCgg                                                                                          <  6:101166/149‑1 (MQ=255)
                                                          gTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGc                                                                                      >  4:232148/1‑149 (MQ=255)
                                                              gTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGt                                                                                                                              <  1:287259/105‑1 (MQ=255)
                                                              gTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGt                                                                                                                              >  2:287259/1‑105 (MQ=255)
                                                               tttGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTggg                                                                                 >  2:154438/1‑149 (MQ=255)
                                                                   cccTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCa                                                                              <  3:232148/148‑1 (MQ=255)
                                                                    ccTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCTGCTGGTGGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAgc                                                                            <  1:236995/149‑1 (MQ=255)
                                                                               tcgtcACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACt                                                                 <  1:51265/149‑1 (MQ=255)
                                                                                 gtcACCGACGTTCTTTCCCAGTGTGTGCGAGTCACCCTTTTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTAGGCAGGGCTTCGCGCTGCTCGTCGGTGGCGTGGGCCAGCGCCGCAGCACtgt                                                               <  4:116766/149‑1 (MQ=255)
                                                                                  tcACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACtgtg                                                              >  2:175642/1‑149 (MQ=255)
                                                                                    aCCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTgg                                                             <  3:115244/148‑1 (MQ=255)
                                                                                      cGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGc                                                                                                                                       >  5:14811/1‑72 (MQ=255)
                                                                                      cGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGc                                                                                                                                       <  6:14811/72‑1 (MQ=255)
                                                                                      cGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTggg                                                            >  4:271533/1‑147 (MQ=255)
                                                                                       gACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACtgt                                                               >  3:221069/1‑143 (MQ=255)
                                                                                       gACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACtgt                                                               <  4:221069/143‑1 (MQ=255)
                                                                                                  ccAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTc                                                                                                                             <  1:283888/70‑1 (MQ=255)
                                                                                                  ccAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTc                                                                                                                             >  2:283888/1‑70 (MQ=255)
                                                                                                      ggTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCa                                          >  3:135709/1‑149 (MQ=255)
                                                                                                                  aCCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGc                              >  6:100648/1‑149 (MQ=255)
                                                                                                                   cccTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGcc                             <  1:175642/149‑1 (MQ=255)
                                                                                                                    gcTTGTAGTCCATCAGGTCGTCGATCAGCTGGAAGTCCACGCCCAGATGGTCGCCAAATGTACGCAGGGCTTCGCGCTGCTCGTCGGtt                                                                                        <  5:63375/88‑2 (MQ=255)
                                                                                                                    ccTTGTAGTCCAGCAGGTCTTCGATCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAACGTACGCAGGGCTTCGCGCAGCTCGTCGGTg                                                                                        >  6:63375/1‑89 (MQ=255)
                                                                                                                    ccTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCcg                            >  4:44771/1‑149 (MQ=255)
                                                                                                                          cttCCAGCTGGTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTc                                                  <  7:113244/119‑1 (MQ=255)
                                                                                                                          cttCCAGCTGGTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTc                                                  >  8:113244/3‑121 (MQ=255)
                                                                                                                             ccAGCTGGTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACa                                             <  1:207930/123‑1 (MQ=255)
                                                                                                                             ccAGCTGGTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACa                                             >  2:207930/1‑123 (MQ=255)
                                                                                                                             ccAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGAcc                   >  4:121734/1‑149 (MQ=255)
                                                                                                                              cagcagGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCt                  >  5:322082/1‑149 (MQ=255)
                                                                                                                                gcagGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTcc                <  3:121734/149‑1 (MQ=255)
                                                                                                                                gcagGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTc                 >  8:271024/1‑148 (MQ=255)
                                                                                                                                  agGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCAt              <  5:100648/149‑1 (MQ=255)
                                                                                                                                       gtcgACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGa         >  8:156075/1‑149 (MQ=255)
                                                                                                                                       gtcgACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGa         >  6:23003/1‑149 (MQ=255)
                                                                                                                                             cAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCa                                          <  4:49938/110‑1 (MQ=255)
                                                                                                                                             cAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCa                                          >  3:49938/1‑110 (MQ=255)
                                                                                                                                             cAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCt   >  3:207993/1‑149 (MQ=255)
                                                                                                                                             cAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCt   >  8:3546/1‑149 (MQ=255)
                                                                                                                                              aGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTc  <  3:271533/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTC  >  NC_002947/799705‑799995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑