Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	817,397	G→A	11.6%	A257A (GCC→GCT)	PP_0703 ←	transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	817,397	0	G	A	11.6%	154.6 / 9.3	60	A257A (GCC→GCT)	PP_0703	transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base G (24/29);  new base A (3/4);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

CTGTACTCGACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGCTGAG  >  NC_002947/817251‑817538                                                                                                                                                   |                                                                                                                                               cTGTACTCGACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTgg                                                                                                                                               <  3:89223/148‑1 (MQ=255) cTGTACTCGACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCCGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGc                                                                                                                                              <  2:45558/149‑1 (MQ=255)       tCGACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCACCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCAGTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTc                                                                                                                                        >  1:242125/1‑149 (MQ=255)         gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTc                                                                                                                                        <  1:24158/147‑1 (MQ=255)         gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTc                                                                                                                                        >  2:24158/1‑147 (MQ=255)         gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCg‑g                                                                                                                                     <  1:153433/149‑1 (MQ=255)         gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTATCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCg‑g                                                                                                                                     <  2:71463/149‑1 (MQ=255)         gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTATCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCg‑g                                                                                                                                     >  1:71463/1‑149 (MQ=255)            ccGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑Gc                                                                                                                                    >  1:138166/1‑147 (MQ=255)              gCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGc                                                                                                                                >  3:125577/1‑149 (MQ=255)              gCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGc                                                                                                                                <  5:118590/149‑1 (MQ=255)                         cTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATctgctg                                                                                                                     <  4:29886/149‑1 (MQ=255)                             gggTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGACACCTTCAAGCCCGCCACTTCTGGAGCGAAGCGCAACACGCCAGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCaag                                                                                                                 >  5:99910/1‑147 (MQ=255)                             gggTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACg                                                                                                                 >  7:232554/1‑149 (MQ=255)                                tGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTg                                                                                                              <  1:114887/149‑1 (MQ=255)                                       ttGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCG‑Gc                                                                                                                                    <  2:32168/120‑1 (MQ=255)                                       ttGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCG‑Gc                                                                                                                                    >  1:32168/1‑120 (MQ=255)                                         gCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCt                                                                                                     >  3:163075/1‑149 (MQ=255)                                          cAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCg‑g                                                                                                                                     <  5:68388/116‑1 (MQ=255)                                          cAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCg‑g                                                                                                                                     >  6:68388/1‑116 (MQ=255)                                          cAGGACTTGCCCGGTGGCAGCCAACTGATAGGGGCCTGACACCTTCCAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGGAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCtt                                                                                                    <  6:102129/149‑1 (MQ=255)                                               tttGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTc                                                                                               <  7:278472/149‑1 (MQ=255)                                               tttGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTACAAGACCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTc                                                                                               >  7:216463/1‑149 (MQ=255)                                                     cGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTacac                                                                                                       >  3:159596/1‑135 (MQ=255)                                                     cGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTacac                                                                                                       <  4:159596/135‑1 (MQ=255)                                                     cGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAAccc                                                                                         >  7:252939/1‑149 (MQ=255)                                                          tCAGCCATCTGTTAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCATCAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAgg                                                                                     <  8:216463/148‑1 (MQ=255)                                                                aaCTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGtt                                                                              <  4:125577/149‑1 (MQ=255)                                                                aaCTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGtt                                                                              <  2:138166/149‑1 (MQ=255)                                                                   tGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGGACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTtgtg                                                                           >  5:275847/1‑149 (MQ=255)                                                                    gATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTtgtg                                                                           >  3:97260/1‑148 (MQ=255)                                                                     aTAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTTCa                                                                         <  2:242125/149‑1 (MQ=255)                                                                     aTAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCa                                                                         <  3:293171/149‑1 (MQ=255)                                                                            ccTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTtgtg                                                                           <  6:176663/140‑1 (MQ=255)                                                                            ccTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTtgtg                                                                           >  5:176663/1‑140 (MQ=255)                                                                              tGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAg                                                                                                            >  6:16397/1‑105 (MQ=255)                                                                              tGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAg                                                                                                            <  5:16397/105‑1 (MQ=255)                                                                              tGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAg                                                                                      <  1:195896/127‑1 (MQ=255)                                                                              tGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAg                                                                                      >  2:195896/1‑127 (MQ=255)                                                                                    cTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGaa                                                          <  8:232554/149‑1 (MQ=255)                                                                                     ttCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAt                                                         >  8:31972/1‑149 (MQ=255)                                                                                        aaGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAtgc                                                       <  4:97260/148‑1 (MQ=255)                                                                                        aaGCCCGCCACTTCTGGAGCGCAGCGCAACAGACCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAtgc                                                       <  6:253772/148‑1 (MQ=255)                                                                                         aGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAtgctg                                                     <  6:275847/149‑1 (MQ=255)                                                                                         aGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAtgctg                                                     >  2:231337/1‑149 (MQ=255)                                                                                           cccGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGcc                                                   >  2:45911/1‑149 (MQ=255)                                                                                            ccGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCaa                                                                                            >  5:27769/1‑107 (MQ=255)                                                                                            ccGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCaa                                                                                            <  6:27769/107‑1 (MQ=255)                                                                                                  cTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGc                                                                                                                >  6:156399/1‑81 (MQ=255)                                                                                                  cTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGc                                                                                                                <  5:156399/81‑1 (MQ=255)                                                                                                  cTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCt                                            <  4:163075/149‑1 (MQ=255)                                                                                                        gAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACt                                      >  5:47485/1‑149 (MQ=255)                                                                                                          gcgcagcgcaACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTg                                    <  8:252939/149‑1 (MQ=255)                                                                                                              agcgcaACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCGAGCAAGCGGCATCTGCTGCACGCTGAGTACACCtt                                                                                                    <  1:42338/82‑1 (MQ=255)                                                                                                                          ccGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCgg                                              <  6:135673/123‑1 (MQ=255)                                                                                                                          ccGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCgg                                              >  5:135673/1‑123 (MQ=255)                                                                                                                          ccGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGgc                    <  6:47485/149‑1 (MQ=255)                                                                                                                           cggtcggtAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACTCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGgca                   >  5:257798/1‑149 (MQ=255)                                                                                                                                 gtAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACtt             <  1:231337/149‑1 (MQ=255)                                                                                                                                        ccGAGTTCGCTGGCAAACTCG‑GCAACCGGCATCTGCTCCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGc      >  1:255586/1‑149 (MQ=255)                                                                                                                                            gTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGCTGAg  <  4:39701/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               CTGTACTCGACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGCTGAG  >  NC_002947/817251‑817538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑