| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 817,400 | A→G | 11.6% | F256F (TTT→TTC) | PP_0703 ← | transmembrane sensor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 817,400 | 0 | A | G | 11.6% | 154.2 / 10.5 | 60 | F256F (TTT→TTC) | PP_0703 | transmembrane sensor |
| Reads supporting (aligned to +/- strand): ref base A (25/28); new base G (3/4); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
TCGACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGCTGAGGCCGAGG > NC_002947/817257‑817545 | tCGACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCACCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCAGTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTc > 1:242125/1‑149 (MQ=255) gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTc < 1:24158/147‑1 (MQ=255) gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTc > 2:24158/1‑147 (MQ=255) gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCg‑g < 1:153433/149‑1 (MQ=255) gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTATCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCg‑g < 2:71463/149‑1 (MQ=255) gACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTATCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCg‑g > 1:71463/1‑149 (MQ=255) ccGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑Gc > 1:138166/1‑147 (MQ=255) gCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGc < 5:118590/149‑1 (MQ=255) gCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGc > 3:125577/1‑149 (MQ=255) cTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATctgctg < 4:29886/149‑1 (MQ=255) gggTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGACACCTTCAAGCCCGCCACTTCTGGAGCGAAGCGCAACACGCCAGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCaag > 5:99910/1‑147 (MQ=255) gggTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACg > 7:232554/1‑149 (MQ=255) tGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTg < 1:114887/149‑1 (MQ=255) ttGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCG‑Gc > 1:32168/1‑120 (MQ=255) ttGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTTGCCAGCGAACTCG‑Gc < 2:32168/120‑1 (MQ=255) gCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCt > 3:163075/1‑149 (MQ=255) cAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCg‑g > 6:68388/1‑116 (MQ=255) cAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCg‑g < 5:68388/116‑1 (MQ=255) cAGGACTTGCCCGGTGGCAGCCAACTGATAGGGGCCTGACACCTTCCAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGGAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCtt < 6:102129/149‑1 (MQ=255) tttGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTc < 7:278472/149‑1 (MQ=255) tttGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTACAAGACCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTc > 7:216463/1‑149 (MQ=255) cGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTacac > 3:159596/1‑135 (MQ=255) cGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTacac < 4:159596/135‑1 (MQ=255) cGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAAccc > 7:252939/1‑149 (MQ=255) tCAGCCATCTGTTAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCATCAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAgg < 8:216463/148‑1 (MQ=255) aaCTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGtt < 4:125577/149‑1 (MQ=255) aaCTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGtt < 2:138166/149‑1 (MQ=255) tGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGGACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTtgtg > 5:275847/1‑149 (MQ=255) gATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTtgtg > 3:97260/1‑148 (MQ=255) aTAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTTCa < 2:242125/149‑1 (MQ=255) aTAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCa < 3:293171/149‑1 (MQ=255) ccTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTtgtg < 6:176663/140‑1 (MQ=255) ccTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTtgtg > 5:176663/1‑140 (MQ=255) tGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAg > 6:16397/1‑105 (MQ=255) tGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAg < 5:16397/105‑1 (MQ=255) tGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAg > 2:195896/1‑127 (MQ=255) tGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAg < 1:195896/127‑1 (MQ=255) cTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGaa < 8:232554/149‑1 (MQ=255) ttCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAt > 8:31972/1‑149 (MQ=255) aaGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAtgc < 4:97260/148‑1 (MQ=255) aaGCCCGCCACTTCTGGAGCGCAGCGCAACAGACCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAtgc < 6:253772/148‑1 (MQ=255) aGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAtgctg > 2:231337/1‑149 (MQ=255) aGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAAtgctg < 6:275847/149‑1 (MQ=255) cccGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGcc > 2:45911/1‑149 (MQ=255) ccGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCaa > 5:27769/1‑107 (MQ=255) ccGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCaa < 6:27769/107‑1 (MQ=255) cTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGc < 5:156399/81‑1 (MQ=255) cTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGc > 6:156399/1‑81 (MQ=255) cTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCt < 4:163075/149‑1 (MQ=255) gAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACt > 5:47485/1‑149 (MQ=255) gcgcagcgcaACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTg < 8:252939/149‑1 (MQ=255) agcgcaACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCGAGCAAGCGGCATCTGCTGCACGCTGAGTACACCtt < 1:42338/82‑1 (MQ=255) ccGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCgg < 6:135673/123‑1 (MQ=255) ccGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCgg > 5:135673/1‑123 (MQ=255) ccGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGgc < 6:47485/149‑1 (MQ=255) cggtcggtAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACTCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGgca > 5:257798/1‑149 (MQ=255) gtAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACtt < 1:231337/149‑1 (MQ=255) ccGAGTTCGCTGGCAAACTCG‑GCAACCGGCATCTGCTCCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGc > 1:255586/1‑149 (MQ=255) gTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGCTGAg < 4:39701/149‑1 (MQ=255) cAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGCTGAGGCCGAgg > 5:44862/1‑147 (MQ=255) | TCGACCCGCACGGGCAGGCTGCGGGTGAGCAGTTGCAGGATTTGCCCGGTGTCAGCCAACTGATAGGTGCCTGAAACCTTCAAGCCCGCCACTTCTGGAGCGCAGCGCAACAGGCCCGGTCGGTAGCGGCCGAGTTCGCTGGCAAACTCG‑GCAAGCGGCATCTGCTGCACGCTGAGTACACCTTCGGTCCAACCCCAGGGGTCGTTGTGCAGTGTTCTGGCCTGAATGCTGCCGTCGGCTGTGACTTGCAAGGTTTCACCGGGCAGCACTTGCCTCGCTGAGGCCGAGG > NC_002947/817257‑817545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |