| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 838,071 | A→G | 6.9% | A209A (GCT→GCC) | rplY ← | 50S ribosomal protein L25/general stress protein Ctc |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 838,071 | 0 | A | G | 6.9% | 169.2 / 2.6 | 58 | A209A (GCT→GCC) | rplY | 50S ribosomal protein L25/general stress protein Ctc |
| Reads supporting (aligned to +/- strand): ref base A (28/26); new base G (2/2); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
GGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGTTGGCCAGGTCGACTTCGAT > NC_002947/837928‑838211 | ggTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAAc > 7:140102/1‑149 (MQ=255) cccAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTg < 8:140102/149‑1 (MQ=255) ggCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGc > 5:306906/1‑149 (MQ=255) ccAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGt < 6:306906/149‑1 (MQ=255) cAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGGGCAACGCGTgg < 5:123218/143‑1 (MQ=255) cAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGGGCAACGCGTgg > 6:123218/1‑143 (MQ=255) aaCGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTAGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTg > 8:222662/1‑148 (MQ=255) aCTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTgg > 3:101153/1‑148 (MQ=255) aCTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTgg > 3:67028/1‑148 (MQ=255) gATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTgg < 4:189470/144‑1 (MQ=255) gATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTgg > 3:189470/1‑144 (MQ=255) cGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCttt < 4:269814/149‑1 (MQ=255) cGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCttt < 4:23640/149‑1 (MQ=255) cGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCttt > 2:235221/1‑149 (MQ=255) cACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGtca < 1:235221/149‑1 (MQ=255) gTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGtcatca < 3:166667/149‑1 (MQ=255) tCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGGGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCAc > 4:309045/1‑149 (MQ=255) ggCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTgg < 6:232041/149‑1 (MQ=255) ttCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTgg > 1:92932/1‑121 (MQ=255) ttCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTgg < 2:92932/121‑1 (MQ=255) ccTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCagag > 2:258681/1‑149 (MQ=255) ccTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCaga < 7:99215/148‑1 (MQ=255) cTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGc > 4:260071/1‑149 (MQ=255) cTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTATGTCATCACCGTGGGCCagacc > 5:163403/1‑147 (MQ=255) gAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGcgac > 3:270634/1‑148 (MQ=255) gAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGcgac < 1:258681/148‑1 (MQ=255) aGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGtt < 1:271829/111‑1 (MQ=255) aGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGtt > 2:271829/1‑111 (MQ=255) aGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGtca < 7:98900/127‑1 (MQ=255) aGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGtca > 8:98900/1‑127 (MQ=255) gtggGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAActc > 5:132123/1‑148 (MQ=255) ttACAGTG‑C‑CGCGCGCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCttt > 3:153416/1‑113 (MQ=255) ttACAGTG‑C‑CGCGCGCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCttt < 4:153416/113‑1 (MQ=255) cAGTGCCGCGC‑‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCttt > 3:3893/1‑149 (MQ=255) tG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCg > 1:154836/1‑148 (MQ=255) ccgcgc‑‑gCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGa < 4:3893/147‑1 (MQ=255) cgcgcg‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGa < 3:260071/148‑1 (MQ=255) gCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCa < 7:10322/149‑1 (MQ=255) aaCCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGa > 7:178262/1‑149 (MQ=255) ccGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGGGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAg > 7:84770/1‑149 (MQ=255) gATCAACGCCGGCTTGCGAGTTGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAAAGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGGCCGACAGGt < 5:182074/149‑1 (MQ=255) gATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGt < 8:178262/149‑1 (MQ=255) tCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTg > 8:71995/1‑148 (MQ=255) aaCGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGgat > 5:149690/1‑149 (MQ=255) ccGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGatgatg < 7:222662/149‑1 (MQ=255) ggTGCTAGTGGATTACTGGGCAGCGCCTTGTGCTTCTGGAGCAACGCGTGTAGCGTGAACGTTGGCAACAGCTTTGTCATCACGGTGTGCCAGAGCGACGAAGTCTACTCCTTTCGGAGCTTTGAGGTCCGACAGGTGGATGATTGTGc < 6:163403/148‑1 (MQ=255) gCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGa > 4:303446/1‑149 (MQ=255) cGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGGGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGAt < 3:309045/149‑1 (MQ=255) cGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTAGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGa < 4:270634/148‑1 (MQ=255) tGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGAttt > 1:116099/1‑147 (MQ=255) gATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGc < 6:149690/149‑1 (MQ=255) aCTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGGGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGtt < 8:84770/148‑1 (MQ=255) cTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGTTg < 2:116099/148‑1 (MQ=255) tCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAAGTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGTTGGc > 7:97549/1‑149 (MQ=255) gcCTTCTGCTTCTGGAGCAACGCGAGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGTTGGCCAGGTCGa > 7:264378/1‑149 (MQ=255) ctgcttctgGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGTTGGCCAGGTCGACTTc > 6:6474/1‑148 (MQ=255) ggAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGTTGGCCAGGTCGACTTCGAt > 8:211640/1‑143 (MQ=255) ggAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGTTGGCCAGGTCGACTTCGAt < 7:211640/143‑1 (MQ=255) | GGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTG‑CGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGAGCAACGCGTGGAGCGTGAACGTTGGCAACAGCTTTGTCATCACCGTGGGCCAGAGCGACGAACTCTACGCCTTTCGGAGCTTTCAGGTCCGACAGGTGGATGATGGTGCCGATTTCAGCGTTGGCCAGGTCGACTTCGAT > NC_002947/837928‑838211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |