Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 839,878 C→A 7.8% R275L (CGT→CTT)  ispE ← 4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947839,8780CA 7.8% 229.9 / 6.9 73R275L (CGT→CTT) ispE4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol kinase
Reads supporting (aligned to +/- strand):  ref base C (36/31);  new base A (2/4);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TATAAGGAAAATGGCAGGGGCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGTTAGTCGAGCCTCAGTGAA  >  NC_002947/839735‑840023
                                                                                                                                               |                                                                                                                                                 
tataAGGAAAATGGCAGGGGCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGc                                                                                                                                              <  4:135203/149‑1 (MQ=255)
   aaGGAAAATGGCAGGGGCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCATCGTAAGCTGCaaa                                                                                                                                           <  5:46020/149‑2 (MQ=255)
    aGGAAAATGGCAGGGGCGGCGGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCGGCGAACGCGCATGCGCTCGACTTCTTGACCAGACTTTGCATCGTAAGCTGCaaa                                                                                                                                           <  5:41314/148‑2 (MQ=18)
      gAAAATGGCAGGGGCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATc                                                                                                                                        >  8:238873/1‑149 (MQ=255)
         aaTGGCAGGGGCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCATCGTAAGCTGCaaagtctgg                                                                                                                                     <  8:289840/149‑8 (MQ=21)
          aTGGCAGGGGCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGaaa                                                                                                                                    >  5:17961/1‑149 (MQ=255)
           tGGCAGGGGCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGaaa                                                                                                                                    >  3:282056/1‑148 (MQ=255)
                ggggCGGCTGGATTCGAACCTACGCATGGCAGGATCAAAACCTGCTGCCTTACCTCTTGGCGACGCCCCTGTAGCTGGTGCTGCGAACGCTCATTCTCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGc                                                                                                                              <  4:219271/149‑1 (MQ=25)
                 gggCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCt                                                                                                                             <  2:232981/149‑1 (MQ=255)
                          gATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGa                                                                                                                                                 <  8:146433/120‑1 (MQ=255)
                          gATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGa                                                                                                                                                 >  7:146433/1‑120 (MQ=255)
                          gATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTgg                                                                                                                     <  1:292402/148‑1 (MQ=255)
                          gATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGc                                                                                                                    <  4:282056/149‑1 (MQ=255)
                            ttCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCATCGTAAGCTGCaaagtc                                                                                                                                        >  7:294325/1‑123 (MQ=25)
                            ttCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCATCGTAAGCTGCaaagtc                                                                                                                                        <  8:294325/127‑5 (MQ=25)
                            ttCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCca                                                                                                                  <  3:283573/149‑1 (MQ=255)
                            ttCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCca                                                                                                                  >  4:159026/1‑149 (MQ=255)
                               gAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCcaca                                                                                                                >  3:304739/1‑148 (MQ=255)
                               gAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCcaca                                                                                                                >  4:306954/1‑148 (MQ=255)
                                    aaCGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGc                                                                                                                    >  7:57335/1‑139 (MQ=255)
                                    aaCGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGc                                                                                                                    <  8:57335/139‑1 (MQ=255)
                                     aCGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTg                                                                                                         >  6:239933/1‑149 (MQ=255)
                                     aCGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTg                                                                                                         <  3:283797/149‑1 (MQ=255)
                                      cGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGt                                                                                                        >  6:96000/1‑149 (MQ=255)
                                          tGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTgg                                                                                                    <  7:32565/149‑1 (MQ=255)
                                          tGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTgg                                                                                                    <  8:68550/149‑1 (MQ=255)
                                            gCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGt                                                                                                  <  5:239933/149‑1 (MQ=255)
                                            gCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGt                                                                                                  <  3:159026/149‑1 (MQ=255)
                                             cAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTTCGATGCAACATCGAAACATTGCTTCCTTTg                                                                                                                      <  1:28615/128‑1 (MQ=255)
                                             cAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTg                                                                                                                      >  2:28615/1‑128 (MQ=255)
                                              aGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAATGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCg                                                                                                >  8:103061/1‑149 (MQ=255)
                                                  tCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGa                                                                                            <  6:180939/149‑1 (MQ=255)
                                                   cAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTCTTGCTGCGAACGCTCATGCGCTCGACTTCTTCACCAGACTTTGCAGCTTACGATGCAAAATCGAAACATAGCTTCCTTTGGCCACAAACCCACTTTGGGTCGCTGa                                                                                            >  2:111227/1‑148 (MQ=38)
                                                       aCCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAaagaa                                                                                       <  4:304739/149‑1 (MQ=255)
                                                         ctgctgCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAgg                                                                                     <  3:92376/149‑1 (MQ=255)
                                                              gcCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGACACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGc                                                                                   >  4:43305/1‑146 (MQ=255)
                                                              gcCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAgg                                                                                     <  3:104011/144‑1 (MQ=255)
                                                              gcCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAgg                                                                                     >  4:104011/1‑144 (MQ=255)
                                                                           gCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCa                                                                      >  3:99383/1‑146 (MQ=255)
                                                                           gCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCa                                                                      <  4:99383/146‑1 (MQ=255)
                                                                                  cccTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAaagaag                                                                                      >  5:195846/1‑123 (MQ=255)
                                                                                  cccTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAaagaag                                                                                      <  6:195846/123‑1 (MQ=255)
                                                                                  cccTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCtt                                                            >  7:15595/1‑149 (MQ=255)
                                                                                  cccTCTATCTGTTGCTGCGAACGCTCATGCGCTCCACTTCTTCACCAGACTTTGCAGCTTACGATGCAACATCCAAACATTGCTGCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCtt                                                            >  4:196337/1‑149 (MQ=255)
                                                                                      gTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCt                                                        >  7:187427/1‑149 (MQ=255)
                                                                                       tATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCtt                                                       <  4:149766/149‑1 (MQ=255)
                                                                                         tCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTgg                                                     >  5:145029/1‑149 (MQ=255)
                                                                                          cTGTTGCTGCGAACGCTCATGCGCTCGACTTGTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCATAACTTTATCAGCTTCGGCTTTGCTTgg                                                     <  1:111227/148‑1 (MQ=255)
                                                                                            gTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTAt                                                                        >  8:3068/1‑127 (MQ=255)
                                                                                            gTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTAt                                                                        <  7:3068/127‑1 (MQ=255)
                                                                                             ttGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGaa                                                  <  7:238873/148‑1 (MQ=255)
                                                                                               gctgcGAATGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAgg                                                <  7:103061/148‑1 (MQ=255)
                                                                                               gctgcGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAgg                                                <  5:96000/148‑1 (MQ=255)
                                                                                                 tgcGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAg                                                 <  1:93256/145‑1 (MQ=255)
                                                                                                 tgcGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAg                                                 >  2:93256/1‑145 (MQ=255)
                                                                                                 tgcGAACGCTCATGCGCTCGACTTATTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTTGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGccc                                             >  6:277486/1‑149 (MQ=255)
                                                                                                   cGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGGTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGccc                                             <  8:56144/147‑1 (MQ=255)
                                                                                                     aaCGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCaaa                                         <  8:187427/149‑1 (MQ=255)
                                                                                                            aTGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACaa                                  >  5:274378/1‑149 (MQ=255)
                                                                                                                gcTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTTGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGCCTTTGCTTGGGAAGGCCCCAAACACACAACTTc                              >  5:136000/1‑149 (MQ=255)
                                                                                                                gcTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTc                              >  4:25193/1‑149 (MQ=255)
                                                                                                                    gACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCa                            <  6:274378/147‑1 (MQ=255)
                                                                                                                    gACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTACTTTTGCGACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCa                            <  6:17961/147‑1 (MQ=255)
                                                                                                                          ttGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGt                    >  6:96238/1‑149 (MQ=255)
                                                                                                                            gACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGTTa                  >  6:286507/1‑149 (MQ=255)
                                                                                                                              ccAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGATGGCGCCAAACACACAACTTCGAGTGCCGGTTAGt                >  4:176788/1‑149 (MQ=255)
                                                                                                                              ccAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGTTAGt                >  7:189067/1‑149 (MQ=255)
                                                                                                                               cAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACCCACAACTTCCAGTGCCGGTTAGTc               >  2:261643/1‑149 (MQ=255)
                                                                                                                               cAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGTTAGTc               >  2:211971/1‑149 (MQ=255)
                                                                                                                               cAGACTTTGCAGCTTAAGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACAGAAGTTCCAGTGCCGGTTAGTc               >  4:257655/1‑149 (MQ=255)
                                                                                                                               cAGACTTAGCAGCTTACGATGCAACATCGAAACATAGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGTTAg                 >  5:295813/1‑147 (MQ=255)
                                                                                                                                    tttGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGTTAGTCGAGcc          >  7:291167/1‑149 (MQ=255)
                                                                                                                                           cTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGTTAGTCGAGCCTCAGTg    >  4:202300/1‑148 (MQ=255)
                                                                                                                                              tcGATGCAACATAGAAACTTTGCTTCGTTTGGCCTCAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGTACTTTATCTGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCGAGTGCCGGTTAGTCGTGCCTCAGTGaa  <  6:27147/146‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TATAAGGAAAATGGCAGGGGCGGCTGGATTCGAACCAACGCATGGCAGGATCAAAACCTGCTGCCTTACCGCTTGGCGACGCCCCTGTATCTGTTGCTGCGAACGCTCATGCGCTCGACTTCTTGACCAGACTTTGCAGCTTACGATGCAACATCGAAACATTGCTTCCTTTGGCCACAAACCCTGTTTGGGTCGCTGAAAGAAGGGCCAGAACTTTATCAGCTTCGGCTTTGCTTGGGAAGGCCCCAAACACACAACTTCCAGTGCCGGTTAGTCGAGCCTCAGTGAA  >  NC_002947/839735‑840023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑