| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 889,448 | G→T | 10.2% | intergenic (+185/‑9) | PP_0769 → / → PP_0770 | sensor histidine kinase/PemI‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 889,448 | 0 | G | T | 10.2% | 144.5 / 6.2 | 49 | intergenic (+185/‑9) | PP_0769/PP_0770 | sensor histidine kinase/PemI‑like protein |
| Reads supporting (aligned to +/- strand): ref base G (24/20); new base T (2/3); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
TGGCTTCCCAAGGTGATGTAGGGCATTTCCTTCTTGAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAACCCGTCA > NC_002947/889306‑889595 | tGGCTTCCCAAGGTGATGTAGGGCATTTCCTTCTTGAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATa < 6:21665/148‑1 (MQ=255) cTTCCCAAGGTGATGTAGGGCATTTCCTTCTTGAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTCCCAAGGTAGATAcata < 5:141572/149‑4 (MQ=255) cTTCCCAAGGTGATGTAGGGCATTTCCTTCTTGAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTCCCAAGGTAGATAcata < 1:263225/149‑4 (MQ=255) cTTCCCAAGGTGATGTAGGGCATTTCCTTCTTGAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCa < 3:278807/149‑1 (MQ=255) gTAGGGCATTTCCTTCTTGAGGTTGCAGGGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTggg > 1:13917/1‑127 (MQ=255) gTAGGGCATTTCCTTCTTGAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTggg < 2:13917/126‑1 (MQ=255) gCATTTCCTTCTTGAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACTTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCaa > 4:129235/1‑147 (MQ=255) cttgttTAGGTTGCA‑GGGCTTTTGTTCTGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGc < 8:219468/149‑1 (MQ=255) gAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAAc < 3:129235/146‑1 (MQ=255) aggCTTTTGTTCGGGCAGGGCAGATGACAGTTTAGATACGAAGTTTCTACATTTCGCTTGCAATCATTTGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGTGGCAACAGCGCCGCGATc < 2:284708/148‑1 (MQ=255) ttttGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCt > 3:174238/1‑149 (MQ=255) ttCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGccc > 1:195917/1‑149 (MQ=255) gTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGc < 1:47579/139‑1 (MQ=255) gTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGc > 2:47579/1‑139 (MQ=255) ggCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGcgccgc > 5:291031/1‑127 (MQ=255) ggCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGcgccgc < 6:291031/127‑1 (MQ=255) ggCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGcgccgc > 7:43663/1‑127 (MQ=255) ggCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGcgccgc < 8:43663/127‑1 (MQ=255) aGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTc < 7:115155/149‑1 (MQ=255) tGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGTGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCaa < 4:174238/148‑1 (MQ=255) gACAGTGTAGATACGACGTTTCTACATTTCCCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAg > 2:180009/1‑148 (MQ=255) acgacgTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCg > 1:102898/1‑149 (MQ=255) gacgTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGgt > 1:44389/1‑149 (MQ=255) tACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTc > 5:196454/1‑149 (MQ=255) aCATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCTGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTcaaggc > 4:185031/1‑143 (MQ=255) aCATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGCGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTc > 2:208129/1‑148 (MQ=255) aTTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCAc > 2:115381/1‑149 (MQ=255) gCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGa < 6:196454/149‑1 (MQ=255) gCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGAAGCGCCTCGGTGTCGGCTCCACCCTGAGc < 1:180009/147‑1 (MQ=255) aaTCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCg < 6:19146/125‑1 (MQ=255) aaTCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCg > 5:19146/1‑125 (MQ=255) aaTCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCtt > 7:153834/1‑148 (MQ=255) aaTCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCtt > 4:264803/1‑148 (MQ=255) aTCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGa < 1:115381/149‑1 (MQ=255) aTCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGa > 3:237388/1‑149 (MQ=255) ggAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACg < 2:44389/149‑1 (MQ=255) gAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCaa > 1:12520/1‑102 (MQ=255) gAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCaa < 2:12520/102‑1 (MQ=255) gAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACgg > 2:55854/1‑149 (MQ=255) aGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGa < 4:175170/49‑1 (MQ=255) aGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGa > 3:175170/1‑49 (MQ=255) tgcatgagTATCTCCCAAGGTAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGAc > 1:293429/8‑148 (MQ=255) ctaTGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATg > 2:113014/1‑149 (MQ=255) tgagTATCTCCCAAGGTAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGAc > 7:10298/4‑144 (MQ=255) tgagTATCTCCCAAGGTAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGAc < 8:10298/141‑1 (MQ=255) tGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATgg < 2:102898/147‑1 (MQ=255) aTCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAg > 7:190987/1‑118 (MQ=255) aTCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAg < 8:190987/118‑1 (MQ=255) cTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAAccc < 3:264803/149‑1 (MQ=255) cTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAAccc > 1:314214/1‑149 (MQ=255) ttGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAAccc > 2:262500/1‑148 (MQ=255) gggAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAACCCGt > 3:148737/1‑148 (MQ=255) ggAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAACCCGTc < 4:148737/148‑1 (MQ=255) ggAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAACCCGTCa < 2:195917/149‑1 (MQ=255) ggAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAACCCGTCa < 1:113014/149‑1 (MQ=255) | TGGCTTCCCAAGGTGATGTAGGGCATTTCCTTCTTGAGGTTGCA‑GGGCTTTTGTTCGGTCAGGGCAGATGACAGTGTAGATACGACGTTTCTACATTTCGCTTGCAATCATTGGGAGGGTTTCTACTATGTATCTACCTTGGGAGATACTCATGCAGATCAAGATTCAACAGTGGGGCAACAGCGCCGCGATCCGCTTGCCCGCCGCAGTACTCAAGCAGATGCGCCTCGGTGTCGGCTCCACCCTGAGCCTTGACACAACGGGTGAGACGATGGTGCTCAAACCCGTCA > NC_002947/889306‑889595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |