Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 138,950 T→G 8.7% R518S (AGA→AGC) ‡ kinB ← alginate biosynthesis sensor protein KinB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947138,9500TG 8.7% 151.9 / 5.4 55R518S (AGA→AGC) ‡kinBalginate biosynthesis sensor protein KinB
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base G (2/3);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

CAATTTCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATCTGCTT  >  NC_002947/138802‑139096
                                                                                                                                                    |                                                                                                                                                  
caATTTCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGt                                                                                                                                                    <  1:315481/149‑1 (MQ=255)
   tttCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTg                                                                                                                                                 <  1:23666/149‑1 (MQ=255)
           cacaGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGGACGGCATGCAg                                                                                                                                         <  6:295663/149‑1 (MQ=255)
           cacaGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCAGACGGCATGCa                                                                                                                                          <  7:153698/148‑1 (MQ=255)
            acaGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAgg                                                                                                                                        >  2:97110/1‑149 (MQ=255)
                  cgAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATg                                                                                                                                  >  8:298237/1‑149 (MQ=255)
                    aGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATgcg                                                                                                                                <  7:298237/149‑1 (MQ=255)
                           gcccagcGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATACCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCccgccg                                                                                                                         <  4:35078/149‑1 (MQ=255)
                             ccagcGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGtt                                                                                                                       >  5:36989/1‑149 (MQ=255)
                             ccagcGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGtt                                                                                                                       >  7:148055/1‑149 (MQ=255)
                                       cTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATgg                                                                                                             <  3:151222/149‑1 (MQ=255)
                                       cTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATgg                                                                                                             <  8:148055/149‑1 (MQ=255)
                                              gCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATg                                                                                                       <  7:157011/148‑1 (MQ=255)
                                                  cctacctGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATgcg                                                                                                                                >  6:279844/1‑119 (MQ=255)
                                                  cctacctGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATgcg                                                                                                                                <  5:279844/119‑1 (MQ=255)
                                                       ctGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATgg                                                                                                      >  2:60000/1‑140 (MQ=255)
                                                       ctGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATgg                                                                                                      <  1:60000/140‑1 (MQ=255)
                                                        tGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTgcag                                                                                            >  8:92540/1‑149 (MQ=255)
                                                                    tCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAg                                                                                >  2:157940/1‑149 (MQ=255)
                                                                                gcTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCaa                                                                    >  8:203671/1‑149 (MQ=255)
                                                                                     ttgCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTgg                                                                <  7:203671/148‑1 (MQ=255)
                                                                                     ttgCCCATAGGAAATGCCCTATCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTgg                                                                <  1:97110/148‑1 (MQ=255)
                                                                                       gCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGAAGGTTGTCCAGCACTCGGTCCAACTGGGc                                                              >  8:248517/1‑148 (MQ=255)
                                                                                        cccATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGa                                                            >  4:262333/1‑149 (MQ=255)
                                                                                         ccATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGAc                                                           >  6:154654/1‑149 (MQ=255)
                                                                                          cATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACg                                                          >  7:218921/1‑149 (MQ=255)
                                                                                           aTAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGt                                                         >  3:135251/1‑149 (MQ=255)
                                                                                             aGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTg                                                                                     <  5:139267/119‑1 (MQ=255)
                                                                                             aGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTg                                                                                     >  6:139267/1‑119 (MQ=255)
                                                                                               gAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTc                                                                       <  6:48392/131‑1 (MQ=255)
                                                                                               gAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTc                                                                       >  5:48392/1‑131 (MQ=255)
                                                                                                   tGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTgg                                                 <  8:218921/149‑1 (MQ=255)
                                                                                                      ccTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATa                                              >  2:282543/1‑149 (MQ=255)
                                                                                                       ctctCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATAc                                             >  4:92547/1‑149 (MQ=255)
                                                                                                                   cTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAgcggcg                                 >  1:161793/1‑149 (MQ=255)
                                                                                                                   cTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAgcggc                                  <  3:262333/148‑1 (MQ=255)
                                                                                                                   cTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGAGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAgcggcg                                 >  7:206121/1‑149 (MQ=255)
                                                                                                                    tCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGcggcgg                                >  6:185365/1‑149 (MQ=255)
                                                                                                                      gACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGcggcgg                                <  1:282543/147‑1 (MQ=255)
                                                                                                                            gatgatCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGtt                        >  5:35571/1‑149 (MQ=255)
                                                                                                                               gatCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCt                     >  3:304148/1‑149 (MQ=255)
                                                                                                                                atCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCt                     <  5:185365/148‑1 (MQ=255)
                                                                                                                                atCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCt                     <  7:92540/148‑1 (MQ=255)
                                                                                                                                 tCACCTGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCtt                    >  2:260234/1‑148 (MQ=255)
                                                                                                                                 tCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCtt                    <  6:36989/148‑1 (MQ=255)
                                                                                                                                 tCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTg                   <  1:157940/149‑1 (MQ=255)
                                                                                                                                 tCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTGGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCtt                    <  4:242270/148‑1 (MQ=255)
                                                                                                                                     ccGTTATGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGGTCCTTGATc                >  2:62802/1‑148 (MQ=255)
                                                                                                                                        gccTGCATGCCAGACGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGcggcgg                                <  2:298740/127‑1 (MQ=255)
                                                                                                                                        gccTGCATGCCAGACGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGcggcgg                                >  1:298740/3‑129 (MQ=255)
                                                                                                                                          cTGCATGCCAGACGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCGAACTGGGCGACGTCGGGCTGGATACGCGGTAGCGGCTGGTACAGTTCCTTGATCAACtg           >  2:114030/1‑147 (MQ=255)
                                                                                                                                             cATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATc       <  7:248517/149‑1 (MQ=255)
                                                                                                                                              aTGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATCt      >  8:37277/1‑149 (MQ=255)
                                                                                                                                                  cGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGa         >  5:131120/1‑142 (MQ=255)
                                                                                                                                                  cGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGa         <  6:131120/142‑1 (MQ=255)
                                                                                                                                                  cGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATCTGCtt  <  6:159403/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAATTTCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATCTGCTT  >  NC_002947/138802‑139096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑