Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	895,201	T→C	11.9%	intergenic (+41/+23)	PP_0777 → / ← PP_0778	glutathione peroxidase/group 1 family glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	895,201	0	T	C	11.9%	173.8 / 12.5	68	intergenic (+41/+23)	PP_0777/PP_0778	glutathione peroxidase/group 1 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (26/33);  new base C (3/5);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GAGAAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTC  >  NC_002947/895053‑895348                                                                                                                                                      |                                                                                                                                                     gagaAGTTTCTGGTGTGGTAGGATGGACGGGGGCTGGCGCGGTTCTCGCCGCGCTCGGCGCCGGAGGACCCAGCGGTGGTGCAGGCGATCGAGAAAGGCCTGGCCTGATGTGTGCCTGAGGTGATGTTT‑TGGCACGTGTGAGATCGAGt                                                                                                                                                      <  8:205602/149‑1 (MQ=255) gagaAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGt                                                                                                                                                      >  8:81609/1‑149 (MQ=255)  agaAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTg                                                                                                                                                     >  8:20960/1‑149 (MQ=255)             gtggGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCgcgggcg                                                                                                                                          <  7:81609/149‑1 (MQ=255)              tggGCAAGGATGGCCGGGTGCTGGCGCTTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGATTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCTAGTGCCGCgcgtgcgg                                                                                                                                         <  6:73245/149‑1 (MQ=255)                ggCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGgcggcg                                                                                                                                       >  3:143682/1‑149 (MQ=255)                 gCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGgcgc                                                                                                                                      >  3:9380/1‑149 (MQ=255)                    aGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTgg                                                                                                                                   <  4:143682/149‑1 (MQ=255)                     ggATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGa                                                                                                                                  <  7:20960/149‑1 (MQ=255)                      gATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAt                                                                                                                                 <  1:129431/149‑1 (MQ=39)                          gCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTca                                                                                                                             <  8:120432/149‑1 (MQ=255)                           ccGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTca                                                                                                                             >  4:115360/1‑148 (MQ=255)                            cGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTca                                                                                                                             >  3:102416/1‑147 (MQ=255)                             gggTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTGCAGCGCCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTcaca                                                                                                                           <  1:68043/149‑1 (MQ=25)                             gggTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAg                                                                                                                          <  5:42127/149‑1 (MQ=38)                             gggTGCTGGCGCGGTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAg                                                                                                                          <  3:197505/149‑1 (MQ=255)                                  cTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCt                                                                                                                     <  6:238705/149‑1 (MQ=255)                                         gTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCaaa                                                                                                                <  6:264486/147‑1 (MQ=255)                                         gTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCaaa                                                                                                                >  5:264486/1‑147 (MQ=255)                                         gTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACa                                                                                                              >  4:61451/1‑149 (MQ=255)                                         gTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACa                                                                                                              >  1:246747/1‑149 (MQ=255)                                          tttCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACAt                                                                                                             >  4:31872/1‑149 (MQ=255)                                           ttCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACAtt                                                                                                            >  4:215799/1‑149 (MQ=255)                                            tctcGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCcgc                                                                                                                                                 <  1:106729/111‑1 (MQ=255)                                            tctcGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCcgc                                                                                                                                                 >  2:106729/1‑111 (MQ=255)                                                  cgcgCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAg                                                                                                      >  4:156525/1‑148 (MQ=255)                                                       aCGGCGCCGGATGACCCAGCGGTCGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACgac                                                                                                >  7:229740/1‑147 (MQ=255)                                                         ggCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGcc                                                                                                >  1:213277/1‑147 (MQ=255)                                                           cgcCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGc                                                                                            <  1:278107/149‑1 (MQ=255)                                                            gcCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTTATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCCGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGcc                                                                                           >  6:326711/1‑149 (MQ=255)                                                                gATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTcaca                                                                                                                           >  5:41596/1‑113 (MQ=37)                                                                gATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTcaca                                                                                                                           <  6:41596/113‑1 (MQ=37)                                                                     cccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGGACCa                                                                                  <  6:78203/149‑1 (MQ=255)                                                                     cccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCAc                                                                                    >  5:95839/1‑147 (MQ=255)                                                                      ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                 <  3:31872/149‑1 (MQ=255)                                                                      ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                 <  3:112906/149‑1 (MQ=255)                                                                             tggtgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCg                                                                          <  2:246747/149‑1 (MQ=255)                                                                                   aGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATgc                                                                    >  5:76043/1‑149 (MQ=255)                                                                                    ggCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATgcg                                                                   <  3:215799/149‑1 (MQ=255)                                                                                      ccATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATgcg                                                                   >  7:222707/1‑147 (MQ=255)                                                                                      ccATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATgcg                                                                   <  8:222707/147‑1 (MQ=255)                                                                                         tCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGtt                                                              <  3:156525/149‑1 (MQ=255)                                                                                         tCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGtt                                                              <  5:134427/149‑1 (MQ=255)                                                                                           gagaAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTc                                                             <  6:95839/148‑1 (MQ=255)                                                                                           gagaAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCa                                                            <  4:57117/149‑1 (MQ=255)                                                                                             gaAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGtt                                                              >  3:303625/1‑145 (MQ=255)                                                                                             gaAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGa                                                          <  4:303625/149‑1 (MQ=255)                                                                                               aaGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCCAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGa                                                          >  4:102469/1‑147 (MQ=255)                                                                                                   gatGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATAcc                                                      <  7:47719/145‑1 (MQ=255)                                                                                                         cTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAg                                                                                                                          <  6:168750/73‑1 (MQ=17)                                                                                                         cTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAg                                                                                                                          >  5:168750/1‑73 (MQ=17)                                                                                                          tGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑Ct                                            >  1:296164/1‑149 (MQ=255)                                                                                                            acgagTGCCTGACGTGATGGTA‑TGGCACCTGTGAGCTCGAGGGCCGCCCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑Ct                                            <  8:203259/145‑1 (MQ=39)                                                                                                             agagTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGtt                                         <  2:213277/149‑1 (MQ=255)                                                                                                             agagTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCCGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTTCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGtt                                         <  5:326711/149‑1 (MQ=255)                                                                                                              gagTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTc                                        >  7:29713/1‑149 (MQ=255)                                                                                                                        cGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGc                               <  1:96212/148‑1 (MQ=255)                                                                                                                           gATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCg                             >  4:263750/1‑147 (MQ=255)                                                                                                                           gATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCg                             <  3:263750/147‑1 (MQ=255)                                                                                                                            aTGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGcc                          >  5:310829/1‑149 (MQ=255)                                                                                                                                     gCAGCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTgg                  <  3:230841/149‑1 (MQ=255)                                                                                                                                      cACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCTGCATGCGCTTTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTggc                 <  8:229740/149‑1 (MQ=255)                                                                                                                                          tgtgAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAGGTGC‑CAAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTg            >  1:129126/1‑149 (MQ=25)                                                                                                                                            tgAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCt             <  4:9380/147‑1 (MQ=255)                                                                                                                                            tgAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTg            <  4:102416/148‑1 (MQ=255)                                                                                                                                              agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCa         >  5:222056/1‑149 (MQ=255)                                                                                                                                               gaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACCTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCa         <  1:51647/149‑1 (MQ=255)                                                                                                                                                aTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCAt        <  6:310829/148‑1 (MQ=255)                                                                                                                                                   gAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTg    >  3:78764/1‑149 (MQ=255)                                                                                                                                                     gTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTc  <  3:189056/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GAGAAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAA‑CTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTC  >  NC_002947/895053‑895348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑