Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 897,397 G→T 6.9% D277Y (GAT→TAT) ‡ PP_0779 → methyl‑accepting chemotaxis transducer/sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947897,3970GT 6.9% 186.0 / 4.0 58D277Y (GAT→TAT) ‡PP_0779methyl‑accepting chemotaxis transducer/sensory box protein
Reads supporting (aligned to +/- strand):  ref base G (28/26);  new base T (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

GATGCCCATGGCCGGGTGATCTGGCTGGAGGCCTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGACGGCATCCAGGCGCTGGATACCCAGTCGCG  >  NC_002947/897256‑897545
                                                                                                                                             |                                                                                                                                                    
gATGCCCATGGCCGGGTGATCTGGCTGGAGGCCTCGTACCACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGgcgc                                                                                                                                               <  8:202729/149‑1 (MQ=255)
    cccATGGCCGGGTGATCTGGCTGGAGGCCTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGACATCTGCCGCCtcg                                                                                                                                           <  7:147530/149‑3 (MQ=255)
     ccATGGCCGGGTGATCTGGCTGGAGGCCTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTac                                                                                                                                           >  3:36046/1‑148 (MQ=255)
                    ctggctggAGGCCTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTac                                                                                                                                           >  1:125221/1‑133 (MQ=255)
                       gctggAGGCCTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGt                                                                                                                            <  6:131166/145‑1 (MQ=255)
                       gctggAGGCCTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTAcc                                                                                                                         >  2:269571/1‑148 (MQ=255)
                             ggCCTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCTCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCa                                                                                                                  <  1:218059/149‑1 (MQ=255)
                                cTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTg                                                                                                               >  1:282022/1‑149 (MQ=255)
                                cTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTg                                                                                                               >  1:200857/1‑149 (MQ=255)
                                    tACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCg                                                                                                           <  3:249923/149‑1 (MQ=255)
                                     aCAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTTGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGt                                                                                                                    <  3:235934/139‑1 (MQ=255)
                                     aCAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGt                                                                                                                    >  4:235934/1‑139 (MQ=255)
                                         cccGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAgg                                                                                                      >  5:160680/1‑149 (MQ=255)
                                         cccGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAgg                                                                                                      >  6:94234/1‑149 (MQ=255)
                                         cccGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAgg                                                                                                      >  3:134418/1‑149 (MQ=255)
                                          ccGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGc                                                                                                     >  6:14882/1‑149 (MQ=255)
                                            gATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGTGACATCTGCCGCCt                                                                                                                                             >  5:216605/1‑107 (MQ=255)
                                            gATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGTGACATCTGCCGCCt                                                                                                                                             <  6:216605/107‑1 (MQ=255)
                                                ttCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCg                                                                                                >  7:144966/1‑148 (MQ=255)
                                                 tCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCg                                                                                                >  4:155971/1‑147 (MQ=255)
                                                   gATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAg                                                                                             <  5:94234/148‑1 (MQ=255)
                                                   gATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAg                                                                                             <  5:14882/148‑1 (MQ=255)
                                                         cACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCAc                                                                                      <  2:282022/149‑1 (MQ=255)
                                                         cACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCAc                                                                                      >  2:20870/1‑149 (MQ=255)
                                                         cACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCAc                                                                                      >  6:231863/1‑149 (MQ=255)
                                                                tgCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGAc                                                                                <  4:36046/148‑1 (MQ=255)
                                                                  cTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTg                                                                              <  1:269571/148‑1 (MQ=255)
                                                                    gTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGaa                                                                                              <  6:1640/130‑1 (MQ=255)
                                                                    gTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGaa                                                                                              >  5:1640/1‑130 (MQ=255)
                                                                     tACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCAc                                                                          >  2:172620/1‑149 (MQ=255)
                                                                      aCAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCAcc                                                                         >  5:47277/1‑149 (MQ=255)
                                                                       cAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCAcc                                                                         <  3:155971/148‑1 (MQ=255)
                                                                       cAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCg                                                                        <  6:160680/149‑1 (MQ=255)
                                                                        aaGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGt                                                                       >  5:53982/1‑149 (MQ=255)
                                                                              gtCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCg                                                                 >  5:177517/1‑149 (MQ=255)
                                                                               tCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCAc                                                                          >  7:66733/1‑139 (MQ=255)
                                                                               tCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCAc                                                                          <  8:66733/139‑1 (MQ=255)
                                                                                 aaGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGAt                                                                                                                      <  2:280878/93‑1 (MQ=255)
                                                                                 aaGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGAt                                                                                                                      >  1:280878/1‑93 (MQ=255)
                                                                                             gTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGa                                                        >  5:149246/1‑143 (MQ=255)
                                                                                             gTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGa                                                        <  6:149246/143‑1 (MQ=255)
                                                                                              tGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCg                                                 <  1:20870/149‑1 (MQ=255)
                                                                                                aTCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTTGa                                                <  1:172620/148‑1 (MQ=255)
                                                                                                   aCCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGAGATGCGTGGACTGGAGGCTTCGATGc                                             >  6:36428/1‑148 (MQ=255)
                                                                                                    ccGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGc                                             >  7:56382/1‑147 (MQ=255)
                                                                                                       aCCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCaa                                                                                                        <  5:45778/85‑1 (MQ=255)
                                                                                                       aCCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCaa                                                                                                        >  6:45778/1‑85 (MQ=255)
                                                                                                            gTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGgccgc                                   <  4:207835/149‑1 (MQ=255)
                                                                                                             tCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGgcc                                     >  2:298147/1‑146 (MQ=255)
                                                                                                             tCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGgcc                                     <  1:298147/146‑1 (MQ=255)
                                                                                                              cAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGCCCACCGAAGTGGTCACCCAGACTGTCACCCTGATGCCTGGACTGGAGGCTTCCATGCAAGAGgccgcc                                  >  3:302829/1‑148 (MQ=255)
                                                                                                                           gCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGACGGCATCCAGgc                    <  5:36428/149‑1 (MQ=255)
                                                                                                                                 gCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGAc                               >  4:111547/1‑132 (MQ=255)
                                                                                                                                 gCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGAc                               <  3:111547/132‑1 (MQ=255)
                                                                                                                                 gCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGACGGCATCCAGGCGCTGGa              <  8:144966/149‑1 (MQ=255)
                                                                                                                                   cGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGACGGCATCCAGGCGCTGGATa            <  6:177517/149‑1 (MQ=255)
                                                                                                                                   cGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGACGGCATCCAGGCGCTGGATa            <  4:134418/149‑1 (MQ=255)
                                                                                                                                    taGGCGACATCTGCCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGACGGCATCCAGGCGCTGGATAc           >  3:91813/2‑149 (MQ=255)
                                                                                                                                             gATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGACAGCATCCAGGCGCTGGATACCCAGTcgcg  >  4:50745/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GATGCCCATGGCCGGGTGATCTGGCTGGAGGCCTCGTACAACCCGATCTTCGATGCCCACGATGTGCTGTACAAGGTGGTCAAGTTCGCCACCGTGATCACCGACCAGGTCAACCAGGAGCTGGCAGTGGCCGAGGCGGCAGATGTCGCCTACAACACCTCGCTGGGTACCGATGTCAGTGCTCGCAAGGCCACCGAAGTGGTCACCCAGACTGTCACCGTGATGCGTGGACTGGAGGCTTCGATGCAAGAGGCCGCCGACGGCATCCAGGCGCTGGATACCCAGTCGCG  >  NC_002947/897256‑897545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑