Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 953,557 G→T 9.8% E122D (GAG→GAT)  cyoC → cytochrome bo terminal oxidase subunit III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947953,5570GT 9.8% 174.4 / 9.3 61E122D (GAG→GAT) cyoCcytochrome bo terminal oxidase subunit III
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base T (3/3);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

GAAACGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACGGCA  >  NC_002947/953414‑953705
                                                                                                                                               |                                                                                                                                                    
gAAACGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTcc                                                                                                                                                  <  3:267989/148‑1 (MQ=255)
      cTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAACTCATAGATTTCCATcgcga                                                                                                                                           <  6:85606/149‑5 (MQ=255)
       tGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGTTGAAGGCTTCATCGCTATTGAAATCTATGCGTTCCATCAcat                                                                                                                                           >  4:37974/1‑146 (MQ=255)
         ttCCTGCTGCTGTCCTCGATCACCTTCGGGTTCGCAATGCTGAAGAAGTTCGATTGCAAGAAAGCATGTGTACTGGGCTGTTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTGATCGCGATGGAAATCTATGAGTTACATCACCTGAt                                                                                                                                        <  3:29048/149‑1 (MQ=255)
               ctgctgTCCTCGATCACCTTCGGCGTCGCCATGCTGAAGATGTTCGAAGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGa                                                                                                                                  <  6:269162/149‑1 (MQ=255)
                tgctgTCCTCGATCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAg                                                                                                                                 <  5:258946/149‑1 (MQ=255)
                     tCCTCGATCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCg                                                                                                                                   >  8:274677/1‑142 (MQ=255)
                     tCCTCGATCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCg                                                                                                                                   <  7:274677/142‑1 (MQ=255)
                           aTCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGccc                                                                                                                      <  4:71991/149‑1 (MQ=255)
                            tCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTACATCACCTGATCAGCGAGGGCTTCGGCCcg                                                                                                                     >  4:76147/1‑149 (MQ=255)
                              aCCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCcgca                                                                                                                   <  4:220282/149‑1 (MQ=255)
                                cTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCAcc                                                                                                                                            >  1:111812/1‑122 (MQ=255)
                                cTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCAcc                                                                                                                                            <  2:111812/122‑1 (MQ=255)
                                cTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCcgcagc                                                                                                                 >  8:16582/1‑149 (MQ=255)
                                   cGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCcgcagcg                                                                                                                <  6:225130/147‑1 (MQ=255)
                                     gCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGt                                                                                                            <  7:16582/149‑1 (MQ=255)
                                          gCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCt                                                                                                        <  8:128931/148‑1 (MQ=255)
                                                  gAAGATGTTCGATGGCAAG‑AAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTc                                                                                                                           >  2:258703/1‑120 (MQ=255)
                                                  gAAGATGTTCGATGGCAAG‑AAGCACGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTc                                                                                                                           <  1:258703/120‑1 (MQ=255)
                                                            gATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGc                                                                                     >  6:84640/1‑149 (MQ=255)
                                                                 cAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTgg                                                                                <  8:228699/149‑1 (MQ=255)
                                                                   aGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTggta                                                                              <  3:37974/149‑1 (MQ=255)
                                                                           ggTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCa                                                                      >  2:79335/1‑149 (MQ=255)
                                                                            gtgtACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCAc                                                                     >  5:114162/1‑149 (MQ=255)
                                                                             tgtACTGGGCTGGTTGGCTGTGACCTTGCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTATCCACg                                                                    <  7:137839/149‑1 (MQ=255)
                                                                                aCTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTc                                                                 >  4:158877/1‑149 (MQ=255)
                                                                                aCTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTc                                                                 >  4:163212/1‑149 (MQ=255)
                                                                                    ggCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGc                                                              <  1:79335/148‑1 (MQ=255)
                                                                                    ggCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCa                                                             >  2:210072/1‑149 (MQ=255)
                                                                                    ggCTGGTTGGCTGTGACCTTCATGTTAGCTGCAGGCTTCATAGCGATGGAAATCTATTAGTTCCATAACCTTATAAGATAGGGCTTATGCACGAATAGCAGTGGATTCATTTCGGGCTTATTCGCCCTGGTAGGTACACACGGTCTGCa                                                             >  4:74641/1‑149 (MQ=255)
                                                                                     gCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCAc                                                            <  8:204263/149‑1 (MQ=255)
                                                                                      cTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGcc                                                                                    <  4:265888/124‑1 (MQ=255)
                                                                                      cTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGcc                                                                                    >  3:265888/1‑124 (MQ=255)
                                                                                        ggTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGt                                                          >  8:258799/1‑148 (MQ=255)
                                                                                         gTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGa                                                        >  2:141662/1‑149 (MQ=255)
                                                                                            ggCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGActg                                                     >  4:117418/1‑149 (MQ=255)
                                                                                                        ccTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGAt                                           <  3:76147/147‑1 (MQ=255)
                                                                                                          tGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGcttctt                                                                                        >  3:101229/1‑100 (MQ=255)
                                                                                                          tGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGcttctt                                                                                        <  4:101229/100‑1 (MQ=255)
                                                                                                          tGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTc                                               <  5:107403/141‑1 (MQ=255)
                                                                                                          tGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTc                                               >  6:107403/1‑141 (MQ=255)
                                                                                                            ttCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGa                                      <  6:114162/148‑1 (MQ=255)
                                                                                                            ttCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGAt                                     >  8:120895/1‑149 (MQ=255)
                                                                                                                 tGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCaa                                >  4:89840/1‑149 (MQ=255)
                                                                                                                 tGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCCGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCaa                                >  5:177109/1‑149 (MQ=255)
                                                                                                                    aGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCa                             >  1:113710/1‑149 (MQ=255)
                                                                                                                        ttCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTgg                                                                                <  1:216688/94‑1 (MQ=255)
                                                                                                                        ttCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTgg                                                                                >  2:216688/1‑94 (MQ=255)
                                                                                                                         tcctcccgATTGAAATATATGAGTTACATCACCTGAACAGCGAGGGCATCCGCCCCCAGCGCACTGGCTTCCTGACGGGCGTCTTCGCCCTGGTAGGTACCCACGCACTGCAAGAGACTGATGGTCTGATCTGCATGGCAATCatgat                         >  4:183153/7‑148 (MQ=255)
                                                                                                                             ggtgATGGAACTCATAGATTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGAc                                                       <  4:177529/111‑1 (MQ=255)
                                                                                                                             ggtgATGGAACTCATAGATTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGAc                                                       >  3:177529/4‑114 (MQ=255)
                                                                                                                              gtgATGGAACTCATAGATTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGa                                      >  7:287826/3‑130 (MQ=255)
                                                                                                                              gtgATGGAACTCATAGATTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGa                                      <  8:287826/128‑1 (MQ=255)
                                                                                                                              gtgATGGAACTCATAGATTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTAc                     >  2:78958/3‑147 (MQ=255)
                                                                                                                              gcgATGGAAATCTATGAGTTCCATCACCCGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCa                   <  6:177109/149‑1 (MQ=255)
                                                                                                                               cgATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTAc                     >  5:190474/1‑146 (MQ=255)
                                                                                                                                  tGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATc               <  1:229971/149‑1 (MQ=255)
                                                                                                                                     aaaTCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATcaac            <  5:84640/149‑1 (MQ=255)
                                                                                                                                          tATTAGTTCCATCACCTGATCAGCGAGTGCTTCGGCCCGCATCGCAGTGGCTTCCTGTCGTGCTTCTTCGCCCTTGTAGGTACCCACGGTCTTCACGTGACTTCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGc        <  3:74641/148‑1 (MQ=255)
                                                                                                                                            tGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCGGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACg     >  2:229093/1‑149 (MQ=255)
                                                                                                                                             gAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACgg    >  7:165587/1‑149 (MQ=255)
                                                                                                                                               gTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACGGc   <  1:229093/148‑1 (MQ=255)
                                                                                                                                               gTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACGGCa  >  7:28183/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GAAACGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATGCTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACGGCA  >  NC_002947/953414‑953705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑