| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 955,155 | C→T | 15.5% | intergenic (+109/‑204) | cyoE → / → alaC | protoheme IX farnesyltransferase/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 955,155 | 0 | C | T | 15.5% | 108.4 / 12.2 | 52 | intergenic (+109/‑204) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (23/20); new base T (3/5); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01 | |||||||||||
CGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATT > NC_002947/955013‑955294 | cGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACACCAACTACAACGTTccaggag > 4:33797/1‑143 (MQ=255) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGc < 4:262873/147‑1 (MQ=38) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg < 5:251289/149‑1 (MQ=255) ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCgg < 8:86430/149‑1 (MQ=37) cTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTAccc < 8:9436/149‑1 (MQ=255) tgatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCAACTAGAAGGTTCCGGTAGGCGCGGGTTTAGCcg > 7:168521/1‑149 (MQ=255) ccTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACACCGCCTCCAAGGGTGCGGGAGGAGCGGGGTTAACCGGGaa > 6:224986/1‑147 (MQ=255) gcacgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTa > 2:111348/1‑122 (MQ=255) gcacgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGa > 4:260971/1‑141 (MQ=255) gcacgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGa < 3:260971/141‑1 (MQ=255) cTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTAGTTTTCCTGTGCCGGACTCTTCGCGGGTAAACCCGCACCAAACAAGTTTCTGGATGACCCGGTTTACCCCCCGATAGGCCAGcac > 7:203660/1‑148 (MQ=255) tGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTAccc < 1:135871/131‑1 (MQ=25) tGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTAccc > 2:135871/1‑131 (MQ=25) cTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCCCTACTACAAGGTTGCTGTAGGAGCGGGTTTTCCCGCGGAGAGGGCCGCACAGaaaaaaaaaaa > 8:244119/1‑148 (MQ=37) tCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTAccc < 5:158257/114‑1 (MQ=18) tCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTAccc > 6:158257/1‑114 (MQ=18) tCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCt > 7:203768/1‑96 (MQ=255) tCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCt < 8:203768/96‑1 (MQ=255) acACGAAACCCCGGCCCCCGGGGCCTGGG‑TTTTTTTTTGGGGGTTTTTTACCTGTGCCGGCCTCTTGGCGGGAAAACCCGCTCCTACACGGTTCCTGTAGTTGGGGGTTAACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCt < 8:168521/147‑1 (MQ=25) gAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACACGCAACTACAAGGTTCCTGTAGGGACGGGTTTAACCGCGAAGAGGCCAGCCCACAAAAAAAAAAATAACTGAatt > 2:217971/1‑149 (MQ=37) gAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACCGCTCCGACAAGGTTGCTGTTGGAGCGGGGTTTGCCGCGAAGCGGCCCGCACAGAAAAATAAAAATAACTGAttt > 1:17962/1‑149 (MQ=38) cGGCCACTGGGCCGGGG‑TTTTTTTTTTGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCATACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCaaaaaa < 3:33797/149‑1 (MQ=255) aCTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGTTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCGCAGAAAAATAAAAATACCTGATTTTCAAAAAATAGAt < 5:295750/149‑1 (MQ=255) tgtgCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTAGCGGGTAAAACCGCTACTACAAGGTTTCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCAAAGAAAAATAAAAATACATGATTTTTAAAAAATAGAtat > 5:7526/1‑147 (MQ=37) cggg‑gTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCCCTCCAACAAGGTTACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCAGAATTTAAAAAAATAGAAATTAAAAg > 6:147668/2‑149 (MQ=25) g‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc < 7:244119/149‑1 (MQ=255) g‑TTTTTTTTTGGGGCTATTTTTCCTGTCCCGGCGTTTTCGCGGGTAACCCCGCTCGTACAAGGTGCCTGTTGGCGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc < 2:214514/149‑1 (MQ=37) tttttttttGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCggg > 8:52506/1‑78 (MQ=255) tttttttttGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCggg < 7:52506/78‑1 (MQ=255) ttttttGGGGGTATTTTTCCTGGCCCGCCCTCTTCGCGGGTAACCCCGCTCCAACACGGTTCCTTTCGTCGCGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaa < 4:137332/148‑1 (MQ=255) ttttttGGGGGCATTTTTCCTGTGCCGGCCTCTTCGCGGGAAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaa < 8:6879/148‑1 (MQ=255) ttttGGTGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCATACAATTTTCCTGTAGTAGCGTGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaata < 5:147668/148‑1 (MQ=37) tGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGTTAAACCCGCTCCTACAAGGTTCCTTTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATaaaa < 2:17962/148‑1 (MQ=255) ggTATTTTTCCTGTGCCGGCCTCTTAGCGGGTTAAACCCCACCAAAAAAGTTGCAGTAGGAGCGGGGTTAACCCCGAAGAGGCCAGCACAGAAAAATAAAAAAAACAGATTTTAAAAAAAAATATCTGAAAAATTATAATAAAACAgg > 4:60584/1‑148 (MQ=255) tcatgtcCCGGCCTCTTCTCGTGTAAACCCGCTCCTACAAGGTTACTTTAGGAGCGGTTTTACCCGCGAAGAGGCCAGCAAAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATc < 5:224986/145‑1 (MQ=37) cGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTTTAGTAGCGTGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAgg < 3:60584/149‑1 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTg > 7:273539/1‑149 (MQ=25) gcgGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGttt > 4:92942/1‑149 (MQ=255) gcgGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGttt > 4:78032/1‑149 (MQ=255) ggTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAc > 7:33450/1‑133 (MQ=255) ggTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAc < 8:33450/133‑1 (MQ=255) aaaCCCGCTACTACAAGGTTCATGATGGATCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGTCa < 1:217971/148‑1 (MQ=255) aCCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTa > 3:126001/1‑149 (MQ=255) ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc > 4:62251/1‑149 (MQ=255) gCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtctt < 8:273539/149‑1 (MQ=38) gCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtctt > 8:255255/1‑149 (MQ=255) cTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAg < 6:278964/122‑1 (MQ=255) cTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAg > 5:278964/1‑122 (MQ=255) tACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt > 3:15425/1‑149 (MQ=255) tACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt > 1:317371/1‑149 (MQ=255) cAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt < 3:78032/148‑1 (MQ=255) tCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCAt < 6:180797/117‑1 (MQ=255) tCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCAt > 5:180797/1‑117 (MQ=255) | CGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATT > NC_002947/955013‑955294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |