Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	971,679	A→G	6.4%	intergenic (+34/‑30)	yajC → / → secD	protein translocase subunit YajC/protein translocase subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	971,679	0	A	G	6.4%	179.0 / 2.5	63	intergenic (+34/‑30)	yajC/secD	protein translocase subunit YajC/protein translocase subunit SecD
Reads supporting (aligned to +/- strand):  ref base A (31/26);  new base G (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

TCAAGGTTTCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGATCAGCGGT  >  NC_002947/971533‑971825                                                                                                                                                    |                                                                                                                                                   tCAAGGTTTCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAAcg                                                                                                                                                  >  2:229502/1‑149 (MQ=255) tCAAGGTTTCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAAcg                                                                                                                                                  >  3:130880/1‑149 (MQ=255)       tttCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGcccc                                                                                                                                            <  6:229227/149‑1 (MQ=255)           cGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGt                                                                                                                                        >  1:147287/1‑149 (MQ=255)                tcTTCGGGGTGGTGGATGTCTCAGACAGTGTCGAGCTGTAGCTCCAGCTGGGCGACATTGCTGCGACCCTGACAAATGTGACGCTCAAGGATATCTGAGTCAGGGTTTTTATTC‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTg                                                                                                                                    <  8:110644/147‑1 (MQ=255)                 cTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGaa                                                                                                                                  >  4:135875/1‑149 (MQ=255)                  ttCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGTTGCGCGCCCcgact                                                                                                                                        <  4:86136/142‑4 (MQ=255)                  ttCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGTTGCGCGCCCcgact                                                                                                                                        >  3:86136/1‑139 (MQ=255)                  ttCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAAc                                                                                                                                 >  5:131596/1‑149 (MQ=255)                  ttCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAAc                                                                                                                                 >  3:9862/1‑149 (MQ=255)                     gtggtgCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACggg                                                                                                                              <  6:131596/149‑1 (MQ=255)                              gAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTCTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGt                                                                                                                        <  1:229502/147‑1 (MQ=255)                               aaGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATg                                                                                                                    >  4:200640/1‑149 (MQ=255)                                 gtgtCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCt                                                                                                                  <  5:121474/149‑1 (MQ=255)                                    tCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTTCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGaa                                                                                                               <  8:295222/149‑1 (MQ=255)                                    tCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGaa                                                                                                               <  7:14661/149‑1 (MQ=255)                                    tCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGa                                                                                                                >  3:141956/1‑148 (MQ=255)                                    tCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGa                                                                                                                <  6:80712/148‑1 (MQ=255)                                            tGTCTAGCTTAATTTCCAGAAGGGTGCCATTGCTGCGACCCGGCCAAAAGGTACGCTCAAGGCTATCTTAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATg                                                                                                                    <  6:131169/136‑1 (MQ=255)                                            tGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATc                                                                                                       >  3:161977/1‑149 (MQ=255)                                            tGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATc                                                                                                       >  3:204696/1‑149 (MQ=255)                                            tGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCGGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATg                                                                                                                    >  5:131169/1‑136 (MQ=255)                                              tCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACgg                                                                                                                               <  7:260817/123‑1 (MQ=255)                                              tCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACgg                                                                                                                               >  8:260817/1‑123 (MQ=255)                                              tCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATc                                                                                                       <  3:200640/147‑1 (MQ=255)                                                   cTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGGCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCtgtg                                                                                                <  6:109615/149‑1 (MQ=255)                                                        gTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAat                                                                                           <  4:130880/149‑1 (MQ=255)                                                           ccAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATg                                                                                        >  8:182889/1‑149 (MQ=255)                                                           ccAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATg                                                                                        >  8:247081/1‑149 (MQ=255)                                                             aGAAGGTTGCCATTGCTGCGACCCTGCAAAAAGGTACGCTCATGGCTATCTTAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCa                                                                                      <  4:141956/149‑1 (MQ=255)                                                                                gACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACtgg                                                                   <  4:161977/149‑1 (MQ=255)                                                                                     tGCCAAAAGGTGCGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTc                                                              >  1:206511/1‑149 (MQ=255)                                                                                     tGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATg                                                                                        >  5:166616/1‑123 (MQ=255)                                                                                     tGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATg                                                                                        <  6:166616/123‑1 (MQ=255)                                                                                      gCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTgg                                                                         <  7:58293/137‑1 (MQ=255)                                                                                      gCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTgg                                                                         >  8:58293/1‑137 (MQ=255)                                                                                         aaaaGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTGTGTGGAAATATGCACTGCTCGGGGGGGTACGGGTGGTCGGtt                                                          >  4:17507/1‑149 (MQ=255)                                                                                         aaaaGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCtgt                                                                                                 >  4:157976/1‑110 (MQ=255)                                                                                         aaaaGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCtgt                                                                                                 <  3:157976/110‑1 (MQ=255)                                                                                         aaaaGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAatat                                                                                         >  3:296973/1‑118 (MQ=255)                                                                                            cggTACGCTCAAGGCTATCTGAGTTACGGTTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCa                                                       <  5:47572/148‑1 (MQ=255)                                                                                            aGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCa                                                       <  3:135875/149‑1 (MQ=255)                                                                                                  gCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTAtt                                                 >  1:303394/1‑149 (MQ=255)                                                                                                   cTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGCGGCGCACCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTc                                                <  2:303394/149‑1 (MQ=255)                                                                                                    tCAAGGCTATCTGAGTTACCGGTATTATTT‑TCCAGTCGGGGAGCTCAAAGCGCCCCGCGTCTAGAACGGGCTGCGTGATGATGAACAAATATACTCTGTGGAAATATGAACTGATCGTGCTGTTATTGGTGGTAGGTTTCATTTAttac                                               >  3:210268/1‑147 (MQ=255)                                                                                                         gCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTcc                                          <  4:204696/149‑1 (MQ=255)                                                                                                          cTATCTGAGTTACCGGTTTTATTT‑TCCCGGCGGGGCGCGAACCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGGCGGTTTCATTTATTCCGCTccc                                         <  4:9862/149‑1 (MQ=255)                                                                                                             tctttgTTTCCGTTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCAGTGATCGTGCTGGTACTGGTTGTCGGTTTCATTTATTCCGATCCCaa                                       <  4:210268/143‑1 (MQ=255)                                                                                                             tCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTAt                                                  <  8:88100/137‑1 (MQ=255)                                                                                                             tCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTAt                                                  >  7:88100/1‑137 (MQ=255)                                                                                                             tCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTCTGGAAATATGCACTGATCGTGCAGGTACTGGTGGTCGGTTTCATTTATTCCGCTACCaa                                       >  3:188831/1‑148 (MQ=255)                                                                                                                gAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCAtt                                                     >  2:118251/1‑131 (MQ=255)                                                                                                                gAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCAtt                                                     <  1:118251/131‑1 (MQ=255)                                                                                                                   ttACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTAc                                >  3:212990/1‑149 (MQ=255)                                                                                                                                tt‑tCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGGTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCcgg                   >  2:40572/1‑149 (MQ=255)                                                                                                                                       gTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTa              >  6:264733/1‑148 (MQ=255)                                                                                                                                       gTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTAc             <  7:182889/149‑1 (MQ=255)                                                                                                                                       cgcGGGGCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACctc                                   >  7:216693/3‑127 (MQ=255)                                                                                                                                       cgcGGGGCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACctc                                   <  8:216693/125‑1 (MQ=255)                                                                                                                                            ggCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTATTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGAt         >  2:198346/1‑148 (MQ=255)                                                                                                                                                cgcAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCg                                                                               >  4:129611/1‑74 (MQ=255)                                                                                                                                                cgcAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCg                                                                               <  3:129611/74‑1 (MQ=255)                                                                                                                                                  cAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGATCAGCGGt  >  6:297493/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TCAAGGTTTCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTT‑TCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGATCAGCGGT  >  NC_002947/971533‑971825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑