Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	147,906	C→T	6.5%	A62T (GCC→ACC)	PP_0139 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	147,906	0	C	T	6.5%	182.6 / 2.1	61	A62T (GCC→ACC)	PP_0139	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (31/26);  new base T (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

TCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAGGCACTGGCCAGGCTCAGCGAG  >  NC_002947/147758‑148054                                                                                                                                                     |                                                                                                                                                      tcAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGc                                                                                                                                                       >  4:299225/1‑149 (MQ=255)    gcgcTCAGGCGCTGTACCTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCaa                                                                                                                                                    <  6:79044/149‑1 (MQ=255)        tCAGGCGCTGTACCTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACt                                                                                                                                                >  7:285497/1‑149 (MQ=255)        tCAGGCGCTGTACCTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACt                                                                                                                                                >  3:254405/1‑149 (MQ=255)        tCAGGCGCTGTACCTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACAGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACt                                                                                                                                                >  3:154055/1‑149 (MQ=255)             cgcTGTACCTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACt                                                                                                                                           >  8:175156/1‑149 (MQ=255)                     cTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCg                                                                                                                                   <  8:285497/149‑1 (MQ=255)                          ccTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCCGCGGCTTg                                                                                                                              >  6:265426/1‑149 (MQ=255)                          ccTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTg                                                                                                                              >  6:50033/1‑149 (MQ=255)                            tCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGtt                                                                                                                            >  4:215729/1‑149 (MQ=255)                             cACGCTGGAACCAGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCCCATCGCTCCAGCGCGCGCCCTAGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGtt                                                                                                                            >  5:144756/1‑148 (MQ=255)                                gCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAgg                                                                                                                        >  8:242824/1‑149 (MQ=255)                                     aaCCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCaa                                                                                                                   <  3:299225/149‑1 (MQ=255)                                      aCCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGCCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAAt                                                                                                                  <  5:211691/149‑1 (MQ=255)                                         cGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑c                                                                                                              <  8:51251/149‑1 (MQ=255)                                          gTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCCGAGCGCGACCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCAAGGAAATCATTGGCCAGGCCAAAACTTCACTGGCCAGCGGCTTGTTGAGTCTCAATGA‑cc                                                                                                             >  4:182557/1‑149 (MQ=255)                                          gTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAg                                                                                                                                     >  7:227636/1‑126 (MQ=255)                                          gTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAg                                                                                                                                     <  8:227636/126‑1 (MQ=255)                                                     ggTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAgcggc                                                                                                  >  5:167181/1‑149 (MQ=255)                                                       ttACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGTCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑c                                                                                                              >  1:297223/1‑135 (MQ=255)                                                       ttACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGTCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑c                                                                                                              <  2:21632/135‑1 (MQ=255)                                                       ttACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGTCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑c                                                                                                              <  2:297223/135‑1 (MQ=255)                                                       ttACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGTCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑c                                                                                                              >  1:21632/1‑135 (MQ=255)                                                               cagcaCCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGc                                                                                        <  3:79635/149‑1 (MQ=255)                                                               cagcaCCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGc                                                                                        <  3:215729/149‑1 (MQ=255)                                                                 gcaCCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCGACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCgg                                                                                      <  2:291153/149‑1 (MQ=255)                                                                    ccGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCggctg                                                                                   <  3:306401/149‑1 (MQ=255)                                                                    ccGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCggctg                                                                                   <  4:254405/149‑1 (MQ=255)                                                                          ccGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGgctgggctgg                                                                             >  5:115361/1‑149 (MQ=255)                                                                              aTCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCa                                                                         >  8:177656/1‑149 (MQ=255)                                                                                     cAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAg                                                                  >  5:303564/1‑149 (MQ=255)                                                                                        cgcgcgcCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGTGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCg                                                               <  6:213715/149‑1 (MQ=255)                                                                                          cgcgcCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGACCCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGa                                                              <  1:233710/149‑1 (MQ=255)                                                                                            cgcCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATaa                                                           <  7:175156/149‑1 (MQ=255)                                                                                               ccTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGa                                                                                                                <  7:138162/94‑1 (MQ=255)                                                                                               ccTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGa                                                                                                                >  8:138162/1‑94 (MQ=255)                                                                                                cTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGAt                                                       >  1:242735/1‑149 (MQ=255)                                                                                                   gTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATc                                                      <  6:115361/147‑1 (MQ=255)                                                                                                      gctgctCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAg                                                 >  3:165304/1‑149 (MQ=255)                                                                                                          ctCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGt                                               <  4:165304/147‑1 (MQ=255)                                                                                                             accacATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCg                                            <  7:177656/147‑1 (MQ=255)                                                                                                               cacaTTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGctct                                        >  4:24212/1‑149 (MQ=255)                                                                                                                  aTTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCg                                     >  5:148130/1‑149 (MQ=255)                                                                                                                  aTTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCg                                     >  7:287387/1‑149 (MQ=255)                                                                                                                     gCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCAGTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGGTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGc                                   <  6:144756/148‑1 (MQ=255)                                                                                                                      cccTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGgctgggctgg                                                                             >  5:118751/1‑105 (MQ=255)                                                                                                                      cccTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGgctgggctgg                                                                             <  6:118751/105‑1 (MQ=255)                                                                                                                      cccTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCa                                  <  4:154055/148‑1 (MQ=255)                                                                                                                       ccTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCCGGCGATAATGATCCACAGGTTCGCTCTGCGGCAg                                 >  4:177326/1‑148 (MQ=255)                                                                                                                        cTGGTGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGTCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGGTCGGCTGGTCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGa                               <  3:182557/149‑1 (MQ=255)                                                                                                                           gggAACCACGGCAATCCTTGGCCCGGCCAACACATCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGAt                              <  6:303564/147‑1 (MQ=255)                                                                                                                               aCCACGGCAATCCTTGGCCCGGCCAACAGTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAg                          >  1:177344/1‑147 (MQ=255)                                                                                                                                 cACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGt                                               >  1:79411/1‑124 (MQ=255)                                                                                                                                 cACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGt                                               <  2:79411/124‑1 (MQ=255)                                                                                                                                 cACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAgg                      >  3:257654/1‑149 (MQ=255)                                                                                                                                  aCGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCg                                            <  1:147600/126‑1 (MQ=255)                                                                                                                                  aCGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCg                                            >  2:147600/1‑126 (MQ=255)                                                                                                                                  aCGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAgg                      >  3:183844/1‑148 (MQ=255)                                                                                                                                      cAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAGGCACTg                 >  4:194223/1‑149 (MQ=255)                                                                                                                                       aaTCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAGGCACTgg                <  3:194223/149‑1 (MQ=255)                                                                                                                                          ccTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAGGCACTGGc               >  6:115364/1‑147 (MQ=255)                                                                                                                                                 ccGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCTGGGTCGAGCAGGCACTGGCCAGGCTccg      >  2:214302/1‑147 (MQ=255)                                                                                                                                                     ccAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAGGCACTGGCCAGGCTCAGCGAg  <  6:167181/149‑1 (MQ=255)                                                                                                                                                     ccAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAGGCACTGGCCAGGCTCAGCGAg  <  5:115364/149‑1 (MQ=255)                                                                                                                                                     ccAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAGGCACTGGCCAGGCTCAGCGAg  >  8:259904/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                      TCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGGAACCCGTCGAGCAAGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTCACCACATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGCCAACACTTCACTGGCCAGCGGCTTGTTCAGGCGCAATGA‑CCAGTCCAGCGGCGGCGCTGTGCGGCTGGGCTGGTTCACCGGCAGGCGATAATGATCCACAGGTTCGCTCTGCGGCAGGATCGAGCAGGCACTGGCCAGGCTCAGCGAG  >  NC_002947/147758‑148054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑