Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,014,084 T→A 7.7% H100L (CAC→CTC)  potF‑I ← putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,014,0840TA 7.7% 147.7 / 3.3 52H100L (CAC→CTC) potF‑Iputrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (25/23);  new base A (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

AGTCCACGGGCGCGTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGTCATCAACTTGCCATCGAGGGTTTCATTGC  >  NC_002947/1013938‑1014222
                                                                                                                                                  |                                                                                                                                          
aGTCCACGGGCGCGTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGc                                                                                                                                          <  8:111973/149‑1 (MQ=255)
   ccACGGGCGCGTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCACTACCGGTACTGCCCCACAGGTACGGGAAGCAGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAg                                                                                                                                       <  6:78177/149‑1 (MQ=255)
   ccACGGGCGCGTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCa                                                                                                                                        <  3:179538/148‑1 (MQ=255)
    cACGGGCGCGTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGt                                                                                                                                      >  7:297107/1‑149 (MQ=255)
     aCGGGCGCGTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACTGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGt                                                                                                                                      >  4:62952/1‑148 (MQ=255)
         gcgcGGTGTCGCCGAGCTCCGCCTTGACCTTGTGGATGTTGTAGCCAATACAGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGTGGCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGc                                                                                                                                 <  6:70809/149‑1 (MQ=255)
             gTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCt                                                                                                                             >  2:73901/1‑149 (MQ=255)
             gTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGACAGTTAGGCAGCt                                                                                                                             >  8:133913/1‑149 (MQ=255)
             gTTGTCGCCGAGCACCGCCTTGACCTTGTCGATCTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCt                                                                                                                             >  5:220381/1‑149 (MQ=255)
              ttGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCtt                                                                                                                            <  1:66250/149‑1 (MQ=255)
                gTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTg                                                                                                                           <  1:73901/148‑1 (MQ=255)
                    ccGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGAttt                                                                                                                      >  6:68257/1‑149 (MQ=255)
                     cGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGAGCCAGTTAGGCAGCTTGGATTTg                                                                                                                     >  3:244932/1‑149 (MQ=255)
                     cGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGAGCCAGTTAGGCAGCTTGGATTTg                                                                                                                     >  3:249247/1‑149 (MQ=255)
                          aCCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAg                                                                                                                >  6:39473/1‑149 (MQ=255)
                                   aCCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCa                                                                                                        <  4:66022/148‑1 (MQ=255)
                                    ccTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAg                                                                                                      <  1:153029/149‑1 (MQ=255)
                                        gTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGACAGCTTGGATTTGTCCAGGCGCTTCAAGGCCt                                                                                                  >  6:300986/1‑149 (MQ=255)
                                         tCGATGTTGTAGCCAATACTGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCtt                                                                                                 <  3:62952/149‑1 (MQ=255)
                                           gATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGc                                                                                               >  2:74533/1‑149 (MQ=255)
                                              gttgtAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCt                                                                                            >  2:150557/1‑149 (MQ=255)
                                               ttgtAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGcc                                                                                              <  1:148017/146‑1 (MQ=255)
                                               ttgtAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGcc                                                                                              >  2:148017/1‑146 (MQ=255)
                                                      cAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGc                                                                                    <  1:150557/149‑1 (MQ=255)
                                                        aTACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGAGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCg                                                                                  <  4:249247/149‑1 (MQ=255)
                                                        aTACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGAGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCg                                                                                  <  4:244932/149‑1 (MQ=255)
                                                           ccGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCggg                                                                                <  6:220381/148‑1 (MQ=255)
                                                            cGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAgg                                                                                                               >  4:161649/1‑116 (MQ=255)
                                                            cGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAgg                                                                                                               <  3:161649/116‑1 (MQ=255)
                                                                       cacaGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGtt                                                                   <  8:297107/149‑1 (MQ=255)
                                                                            gTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAgg                                                              <  5:68257/149‑1 (MQ=255)
                                                                             tACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGACAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAggg                                                             <  5:300986/149‑1 (MQ=255)
                                                                                  gAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAaca                                                        >  6:153811/1‑149 (MQ=255)
                                                                                   aaGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGGTGGAGGGGAacac                                                       <  2:33929/149‑1 (MQ=255)
                                                                                        gTACTGGTTGCCCGGGTCGTTCACCTCCAGCACCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTTGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCAcg                                                   >  2:232196/1‑148 (MQ=255)
                                                                                                   ccGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGaa                                       >  4:129428/1‑149 (MQ=255)
                                                                                                        tCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGcc                                  <  5:39473/149‑1 (MQ=255)
                                                                                                            tCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAg                                                               <  1:293513/116‑1 (MQ=255)
                                                                                                            tCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAg                                                               >  2:293513/1‑116 (MQ=255)
                                                                                                              aCCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGtcat                            >  2:73731/1‑149 (MQ=255)
                                                                                                                cTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGtcatc                           >  7:145040/1‑148 (MQ=255)
                                                                                                                cTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGtcatc                           >  4:175143/1‑148 (MQ=255)
                                                                                                                   cAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGGAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGTCATCAAc                        <  7:133913/148‑1 (MQ=255)
                                                                                                                   cAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCgg                                <  8:212243/140‑1 (MQ=255)
                                                                                                                   cAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCACTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCgg                                >  7:212243/1‑140 (MQ=255)
                                                                                                                         cTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGc                                   >  7:173360/1‑131 (MQ=255)
                                                                                                                         cTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGc                                   <  8:173360/131‑1 (MQ=255)
                                                                                                                           tGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGTCATCAACTTGCCATc                >  5:45193/1‑148 (MQ=255)
                                                                                                                              gcagcaCCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGTCATCAACTTGCCATCGAgg            <  8:145040/149‑1 (MQ=255)
                                                                                                                                       ggTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGTCATCAACTTGCCATCGAGGGTTTCATTg   >  1:39608/1‑149 (MQ=255)
                                                                                                                                        gTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGTCATCAACTTGCCATCGAGGGTTTCATTGc  >  3:138927/1‑149 (MQ=255)
                                                                                                                                        gTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGTCATCAACTTGCCATCGAGGGTTTCATTGc  >  4:138462/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
AGTCCACGGGCGCGTTGTCGCCGAGCACCGCCTTGACCTTGTCGATGTTGTAGCCAATACCGGTACTGCCCCACAGGTACGGGAAGCCGTACTGGTTGCCCGGGTCGTTCACCTCCAGCGCCTTGAGCAGCACCGGGTTGAGGTTGTGCCAGTTAGGCAGCTTGGATTTGTCCAGGCGCTTCAAGGCCTTGCCCTGGATCTGCCGGGCCATGAAATGGTTGGAGGGGAACACCACGTCGTAGCCGGAATTGCCGGTCATCAACTTGCCATCGAGGGTTTCATTGC  >  NC_002947/1013938‑1014222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑