Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,026,485 G→C 9.1% T161T (ACC→ACG)  dppA‑II ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,026,4850GC 9.1% 143.5 / 4.8 50T161T (ACC→ACG) dppA‑IIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base C (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

AACACGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACAT  >  NC_002947/1026339‑1026633
                                                                                                                                                  |                                                                                                                                                    
aaCACGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGt                                                                                                                                                    <  8:270579/149‑1 (MQ=255)
   acGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAgg                                                                                                                                                      >  5:149390/1‑144 (MQ=255)
   acGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAgg                                                                                                                                                      <  6:149390/144‑1 (MQ=255)
     gAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAaca                                                                                                                                               >  6:50111/1‑149 (MQ=255)
         ggCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCACCCTGAAcaccgt                                                                                                                                           <  6:147709/149‑3 (MQ=255)
                  ccGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATg                                                                                                                                   <  5:50111/148‑1 (MQ=255)
                    gATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGggt                                                                                                                                >  7:104175/1‑149 (MQ=255)
                             ctgATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGtcttct                                                                                                                       <  5:179235/149‑1 (MQ=255)
                              tgattgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGggtcg                                                                                                                              >  2:165856/1‑141 (MQ=255)
                              tgattgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGggtcg                                                                                                                              <  1:165856/141‑1 (MQ=255)
                                attgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTtctc                                                                                                                      <  6:252705/147‑1 (MQ=255)
                                attgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTtctc                                                                                                                      >  5:252705/1‑147 (MQ=255)
                                attgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCACCCTGAAcaccgt                                                                                                                                           <  7:55387/126‑3 (MQ=255)
                                attgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCACCCTGAAcaccgt                                                                                                                                           >  8:55387/1‑124 (MQ=255)
                                          cTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCTTATTCAGCGGAGAGGATGGAGGCGCAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTAAGAGAGAACACAACGTTCAATGCGTCGGTCTTCTCGACACGGGCGAt                                                                                                          >  8:193596/1‑149 (MQ=38)
                                              ggCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTc                                                                                                      <  1:301858/149‑1 (MQ=255)
                                               gCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAgg                                                                                                                                                      <  1:169834/100‑1 (MQ=255)
                                               gCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAgg                                                                                                                                                      >  2:169834/1‑100 (MQ=255)
                                                cGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGTGATGTTCtt                                                                                                    <  2:21496/149‑1 (MQ=255)
                                                cGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCtt                                                                                                    <  8:104175/149‑1 (MQ=255)
                                                 gTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTg                                                                                                   >  7:61726/1‑149 (MQ=255)
                                                 gTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTg                                                                                                   >  7:31269/1‑149 (MQ=255)
                                                     aCTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATg                                                                                                                                   <  4:199503/113‑1 (MQ=255)
                                                     aCTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATg                                                                                                                                   >  3:199503/1‑113 (MQ=255)
                                                       tGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTg                                                                                                   >  8:26516/1‑143 (MQ=255)
                                                       tGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTg                                                                                                   <  7:26516/143‑1 (MQ=255)
                                                         gCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGt                                                                                                  >  8:196299/1‑142 (MQ=255)
                                                         gCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGt                                                                                                  <  7:196299/142‑1 (MQ=255)
                                                              tAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGa                                                                                                                    <  5:81735/119‑1 (MQ=255)
                                                              tAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGa                                                                                                                    >  6:81735/1‑119 (MQ=255)
                                                                   gctCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTATCGACACGGGCGAAGTTCTTCTCCAGGCCCAAGCCGtt                                                                                  >  5:261575/1‑148 (MQ=255)
                                                                         cGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAg                                                                           >  1:32072/1‑149 (MQ=255)
                                                                                   ggAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGTAACTCgg                                                                 >  3:298324/1‑149 (MQ=255)
                                                                                        ggATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGTAACTCggtaggg                                                            <  4:298324/149‑1 (MQ=255)
                                                                                        ggATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtaggg                                                            <  2:32072/149‑1 (MQ=255)
                                                                                            ggAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGgtagggtagg                                                        >  5:166092/1‑149 (MQ=255)
                                                                                             gAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGc                                                       <  8:61726/149‑1 (MQ=255)
                                                                                                  gAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCg                                                                                                                     <  6:206054/82‑1 (MQ=255)
                                                                                                       tCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAc                                             >  4:122370/1‑149 (MQ=255)
                                                                                                                       gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTc                               <  3:122370/147‑1 (MQ=255)
                                                                                                                       gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTc                               >  7:91168/1‑147 (MQ=255)
                                                                                                                                ggCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCg                      >  3:291015/1‑147 (MQ=255)
                                                                                                                                ggCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCg                      <  4:291015/147‑1 (MQ=255)
                                                                                                                                   aTCGACGGTGTTCAGGGTGAACACCACGGTCATTGGGTCGGTCTTCTCGACTCGGGCGATGTTCTTGTCCAGGCCCATGCCGTTTAAATAGGGAAACTCGGTATGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGa                 <  7:193596/149‑1 (MQ=255)
                                                                                                                                      gACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGtt                                                                                  >  7:273973/1‑81 (MQ=255)
                                                                                                                                      gACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGtt                                                                                  <  8:273973/81‑1 (MQ=255)
                                                                                                                                      gACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAgg              >  5:88369/1‑149 (MQ=255)
                                                                                                                                      cgtggTGTTCACCCTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGACGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATTACCCTTGTCGAGAATGCGGTTGAAgt              >  6:110068/4‑148 (MQ=255)
                                                                                                                                        cGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCgg                                                                 <  4:4286/96‑1 (MQ=255)
                                                                                                                                        cGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCgg                                                                 >  3:4286/1‑96 (MQ=255)
                                                                                                                                           tgtTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGaa          >  7:131639/1‑148 (MQ=255)
                                                                                                                                             ttCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAACa        <  8:91168/148‑1 (MQ=255)
                                                                                                                                              tCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAACAGc      >  4:222299/1‑149 (MQ=255)
                                                                                                                                               cAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAACAGc      <  6:166092/148‑1 (MQ=255)
                                                                                                                                                  ggTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACAt  >  8:34607/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AACACGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAACAGCACAT  >  NC_002947/1026339‑1026633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑