Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,040,839	A→G	6.9%	L166L (TTG→CTG)	PP_0900 ←	PAP2 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,040,839	0	A	G	6.9%	207.7 / 2.8	72	L166L (TTG→CTG)	PP_0900	PAP2 family protein
Reads supporting (aligned to +/- strand):  ref base A (35/31);  new base G (3/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCCGTCCACACATTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTCCAAGGGCACTGCACCTGGGTGAC  >  NC_002947/1040699‑1040986                                                                                                                                             |                                                                                                                                                    cacTGCCGTCCACACATTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACa                                                                                                                                             <  8:154808/149‑1 (MQ=255) cacTGACGTCCACACATTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTAAAGGCCACTACAAATGCCACTCTCGCCAGGCTTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACa                                                                                                                                             <  1:4420/149‑1 (MQ=255)    tGCCGTCCACACATTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAgg                                                                                                                                           >  4:97871/1‑148 (MQ=255)      ccGTCCACACATTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAgg                                                                                                                                           >  1:120665/1‑146 (MQ=255)           cacacaTTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAAGGCCACTCGCGCCAGGCGTGGCTTGCGAGCACGCCGCAGGAAGCACAGGGCGAAGAGGCAGAAGcc                                                                                                                                   >  8:112761/1‑149 (MQ=255)            acacaTTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCg                                                                                                                                  >  2:50476/1‑149 (MQ=255)             cacaTTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCgg                                                                                                                                 <  2:165559/149‑1 (MQ=255)              acaTTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGCACAAGGCGAACAGGCAGAAACCGGt                                                                                                                                >  2:264836/1‑149 (MQ=255)                   gtgCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCt                                                                                                                            <  2:94615/148‑1 (MQ=255)                    tgCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTg                                                                                                                           >  2:36112/1‑148 (MQ=255)                      cGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCAt                                                                                                                        <  6:175713/149‑1 (MQ=255)                          aGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCTACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATgccc                                                                                                                    >  1:24986/1‑149 (MQ=255)                            gAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATgcccgc                                                                                                                  >  5:168411/1‑149 (MQ=255)                              tttGCTCCCCCTGCATCATCCTGCCCACAGACAGTATCGACGCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCGAGGCGTGGCTTGCGTTCTGGCTTGAGGATGATCATGGCGATCAGGCAGAAACGGGTCGCTGCATgcccggcc                                                                                                                <  4:273751/147‑1 (MQ=255)                                      cccTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGca                                                                                                        <  1:132266/149‑1 (MQ=255)                                       ccTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGTCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGca                                                                                                        >  5:204003/1‑148 (MQ=255)                                         tGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAg                                                                                                     >  1:62031/1‑149 (MQ=255)                                           catcatCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAg                                                                                                     <  1:38027/147‑1 (MQ=255)                                               atcCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAg                                                                                                <  2:62031/148‑1 (MQ=255)                                                tcCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCTACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGc                                                                                              <  2:24986/149‑1 (MQ=255)                                                tcCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCTACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGc                                                                                              >  5:281829/1‑149 (MQ=255)                                                tcCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGc                                                                                              >  7:16454/1‑149 (MQ=255)                                                    gCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGt                                                                                          >  7:278161/1‑149 (MQ=255)                                                    gCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGt                                                                                          >  3:67581/1‑149 (MQ=255)                                                     cccACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGt                                                                                          >  6:113870/1‑148 (MQ=255)                                                     cccACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGt                                                                                          <  5:113870/148‑1 (MQ=255)                                                     cccACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTc                                                                                         <  6:168411/149‑1 (MQ=255)                                                      ccACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCg                                                                                        <  3:97871/149‑1 (MQ=255)                                                          cGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACa                                                                                                                                             <  7:62842/92‑1 (MQ=255)                                                          cGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACa                                                                                                                                             >  8:62842/1‑92 (MQ=255)                                                            aCAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAggc                                                                                  >  8:230398/1‑149 (MQ=255)                                                            aCAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAggc                                                                                  >  3:202191/1‑149 (MQ=255)                                                                  tCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGt                                                                            >  6:263451/1‑149 (MQ=255)                                                                     aCCCTGCTGTCAAGGCTACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTgg                                                                          >  7:8096/1‑148 (MQ=255)                                                                     aCCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGc                                                                         <  7:230398/149‑1 (MQ=255)                                                                        ctgctgTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTcc                                                                      <  1:36112/149‑1 (MQ=255)                                                                              tCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAg                                                                                                          <  8:288315/107‑1 (MQ=255)                                                                              tCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAg                                                                                                          >  7:288315/1‑107 (MQ=255)                                                                                   gCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGaaa                                                                                                                                     <  2:313287/75‑1 (MQ=255)                                                                                   gCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGaaa                                                                                                                                     >  1:313287/1‑75 (MQ=255)                                                                                   gCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCt                                                           >  3:75574/1‑149 (MQ=255)                                                                                       cTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAg                                                       >  5:182279/1‑149 (MQ=255)                                                                                          cAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTa                                                        <  5:268641/145‑1 (MQ=255)                                                                                          cAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTa                                                        >  6:268641/1‑145 (MQ=255)                                                                                          cAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGtt                                                    >  3:256730/1‑149 (MQ=255)                                                                                           aaaTGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGttt                                                   <  8:150967/149‑1 (MQ=255)                                                                                           aaaTGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGttt                                                   >  7:217895/1‑149 (MQ=255)                                                                                             aTGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAGGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGttt                                                   <  4:16068/147‑1 (MQ=255)                                                                                             aTGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAGGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGttt                                                   >  3:16068/1‑147 (MQ=255)                                                                                             aTGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAGGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGttt                                                   <  4:16071/147‑1 (MQ=255)                                                                                             aTGCCACTCGCGCCAGGCGTGGCTTGCGATCACGAAGCAGGAAGAACAGGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGttt                                                   >  3:16071/1‑147 (MQ=255)                                                                                                ccACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGt                                               <  1:50476/148‑1 (MQ=255)                                                                                                  aCTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTgcc                                            <  4:256730/149‑1 (MQ=255)                                                                                                      gcgcCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAAcc                                                                                                                                   >  7:5126/1‑58 (MQ=255)                                                                                                      gcgcCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAAcc                                                                                                                                   <  8:5126/58‑1 (MQ=255)                                                                                                         ccAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGGCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAAc                                     <  6:204003/149‑1 (MQ=255)                                                                                                         ccAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAAc                                     <  4:67581/149‑1 (MQ=255)                                                                                                            ggCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTgg                                  <  7:112761/149‑1 (MQ=255)                                                                                                               gTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGt                                >  6:258684/1‑148 (MQ=255)                                                                                                               gTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGt                                >  2:102420/1‑148 (MQ=255)                                                                                                                    ttGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTgccgc                                          >  5:154824/1‑133 (MQ=255)                                                                                                                    ttGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTgccgc                                          <  6:159395/133‑1 (MQ=255)                                                                                                                    ttGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTgccgc                                          <  6:154824/133‑1 (MQ=255)                                                                                                                    ttGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTgccgc                                          >  5:159395/1‑133 (MQ=255)                                                                                                                       cGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTcc                        <  2:120665/148‑1 (MQ=255)                                                                                                                             cGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTCCAAGGGCa                 >  6:14117/1‑149 (MQ=255)                                                                                                                              gcagcaGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTCCAAGGGCAc                <  4:202191/149‑1 (MQ=255)                                                                                                                              gcagcaGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTCCAAGGGCAc                >  6:193812/1‑149 (MQ=255)                                                                                                                                  cagGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTCCAAGGGCACTGCa            >  3:151874/1‑149 (MQ=255)                                                                                                                                    ggAACAACAACCCGAAAAGCCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCACGCTAGACGTTAGGCGTACCTCGCTCCAGCAGCATGCTGTAGGTTACGGAGCCGCCAAACTGGCCCAGGCTCCAAGGGCACTGCaa           >  4:58998/1‑147 (MQ=255)                                                                                                                                       agaaCAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTCCAAGGGCACTGCACCTgg       <  8:217895/149‑1 (MQ=255)                                                                                                                                         aaCAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTCCAAGGGCACTGCACCTGGGt     <  5:263451/149‑1 (MQ=255)                                                                                                                                            aaGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGACAGGCTCCAAGGGCACTGCACCTGGGTGAc  <  6:281829/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                    CACTGCCGTCCACACATTGTGCGACAGGAAGTGCGCCCCCTGCATCATCCTGCCCACCGACAGTATCGACCCTGCTGTCAAGGCCACTACAAATGCCACTCGCGCCAGGCGTGGCTTGCGATCACGCAGCAGGAAGAACAAGGCGAACAGGCAGAAACCGGTCGCTGCATGCCCGCCCGGCCAGCACAGCCCAGGCTTGTCGGTAGGCGGCCGTGGCTCCAGCAGCTTGCTGTAGGTTTCGGTGCCGCCAAACTGGGTCAGGCTCCAAGGGCACTGCACCTGGGTGAC  >  NC_002947/1040699‑1040986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑