Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 168,379 T→C 6.6% intergenic (‑136/‑25) gcdR ← / → gcdH gcdH transcriptional regulator/glutaryl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947168,3790TC 6.6% 177.8 / 2.0 61intergenic (‑136/‑25)gcdR/gcdHgcdH transcriptional regulator/glutaryl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (25/31);  new base C (2/2);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TTACGGCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATCAGTTCGCCCAGGACAAGCTGG  >  NC_002947/168233‑168518
                                                                                                                                                  |                                                                                                                                           
ttACGGCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCAGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGa                                                                                                                                           >  6:132162/1‑149 (MQ=255)
 tACGGCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGAc                                                                                                                                          >  1:42556/1‑149 (MQ=255)
  aCGGCGCATGTACGTCAACCTCACTTGTGAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGAc                                                                                                                                          <  6:269937/148‑1 (MQ=255)
  aCGGCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGa                                                                                                                                           <  1:37253/147‑1 (MQ=255)
  aCGGCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGa                                                                                                                                           >  2:37253/1‑147 (MQ=255)
  aCGGCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGCGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGa                                                                                                                                           <  5:191768/147‑1 (MQ=255)
    ggCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCTTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACCGAcccc                                                                                                                                       >  8:51033/1‑149 (MQ=255)
    ggCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGAcccc                                                                                                                                       >  7:17562/1‑149 (MQ=255)
    ggCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACCGAccc                                                                                                                                        >  8:55265/1‑148 (MQ=255)
               gTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGa                                                                                                                            <  7:104512/149‑1 (MQ=255)
                    ccTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACATAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCAGAAGAAACTGACCCATATTTCGAGGATTCg                                                                                                                        >  6:100574/1‑148 (MQ=255)
                     cTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCt                                                                                                                      >  6:195585/1‑149 (MQ=255)
                        aCTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTc                                                                                                                     <  7:127322/147‑1 (MQ=255)
                        aCTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCAt                                                                                                                   <  6:158975/149‑1 (MQ=255)
                        aCTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACAGACCCCTATTTCGAGGATTCGCTCa                                                                                                                    <  2:48937/148‑1 (MQ=255)
                                       aaTAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAg                                                                                                    <  3:282850/149‑1 (MQ=255)
                                        aTAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGt                                                                                                   >  3:143977/1‑149 (MQ=255)
                                        aTAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGt                                                                                                   <  6:101323/149‑1 (MQ=255)
                                            ggCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTc                                                                                                >  5:104129/1‑148 (MQ=255)
                                             gCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACCGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCaa                                                                                              <  7:55265/149‑1 (MQ=255)
                                             gCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACCGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCaa                                                                                              <  7:51033/149‑1 (MQ=255)
                                                  ttGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTgg                                                                                          <  1:66659/148‑1 (MQ=255)
                                                            aCGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGtt                                                                               >  6:126899/1‑149 (MQ=255)
                                                                  tGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAAc                                                                                             <  7:210906/129‑1 (MQ=255)
                                                                  tGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAAc                                                                                             >  8:210906/1‑129 (MQ=255)
                                                                    aCAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGctgctg                                                                        <  8:17562/148‑1 (MQ=255)
                                                                    aCAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGctgctg                                                                        >  8:260126/1‑148 (MQ=255)
                                                                      aaaaTATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGAt                                                                     <  7:202508/149‑1 (MQ=255)
                                                                      aaaaTATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGAt                                                                     >  3:307922/1‑149 (MQ=255)
                                                                        aaTATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGAt                                                                     >  6:274973/1‑147 (MQ=255)
                                                                            tCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATcagcag                                                               >  8:151320/1‑149 (MQ=255)
                                                                            tCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATcagca                                                                <  8:172147/148‑1 (MQ=255)
                                                                            tCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATcagca                                                                <  4:307922/148‑1 (MQ=255)
                                                                               cTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCt                                                             >  1:175713/1‑148 (MQ=255)
                                                                               cTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCt                                                             >  1:143526/1‑148 (MQ=255)
                                                                               cTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCt                                                             <  2:143526/148‑1 (MQ=255)
                                                                                tAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTc                                                            >  8:124640/1‑148 (MQ=255)
                                                                                  aTCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACt                                                         <  1:181955/149‑1 (MQ=255)
                                                                                     ggTGCGCAATCCTTGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTgaa                                                      <  5:274973/149‑1 (MQ=255)
                                                                                          gcAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTgaagaa                                                   >  5:91770/1‑147 (MQ=255)
                                                                                            aaTCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAgc                                               <  6:104129/149‑1 (MQ=255)
                                                                                             aTCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGaagaag                                                  <  3:103745/145‑1 (MQ=255)
                                                                                             aTCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGaagaag                                                  >  4:103745/1‑145 (MQ=255)
                                                                                                cTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCa                                            <  5:172620/148‑1 (MQ=255)
                                                                                                cTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCa                                            >  6:172620/1‑148 (MQ=255)
                                                                                                   gTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTgaag                                                     >  1:218579/1‑136 (MQ=255)
                                                                                                   gTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTgaag                                                     <  2:218579/136‑1 (MQ=255)
                                                                                                   gTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATg                                          <  5:126899/147‑1 (MQ=255)
                                                                                                      tGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGc                                      <  5:195585/148‑1 (MQ=255)
                                                                                                       gTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGt                                    >  6:207828/1‑149 (MQ=255)
                                                                                                        tCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGt                                    <  7:260126/148‑1 (MQ=255)
                                                                                                             gcAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAgc                              >  2:139226/1‑149 (MQ=255)
                                                                                                              cAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAgcg                             <  2:42556/149‑1 (MQ=255)
                                                                                                               aGCCGATCAGGCCTAGGCTCAAAGGCACAACAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAATGCAAGTTTCAACTGGATCGACGCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAgcgc                            <  6:226919/149‑1 (MQ=255)
                                                                                                                     tCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATcn                      <  2:175713/149‑2 (MQ=255)
                                                                                                                        ggCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATCAGt                    >  1:128130/1‑148 (MQ=255)
                                                                                                                         gCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATCAGTTc                  <  7:124640/149‑1 (MQ=255)
                                                                                                                                   aaaGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTTGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATCAGTTCGCCCAGGa          <  5:132162/147‑1 (MQ=255)
                                                                                                                                   aaaGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATCAGTTCGCCCAGGa          <  1:139226/147‑1 (MQ=255)
                                                                                                                                     aGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATCAGTTCGCCCAGGACAAg      <  2:128130/149‑1 (MQ=255)
                                                                                                                                         acaAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATCAGTTCGCCCAGGACAAGCTgg  >  2:181805/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
TTACGGCGCATGTACGTCAACCTCACTTGTAAGGCTGTCAATAAGGCGTTTTGCCGATTTACGGAGTGACAAAATATCACTAATCGGTGCGCAATCCTCGTTTGTCGCCGCAGCCGATCAGGCCTAGGCTCAAAGGCACAAGAAACTGACCCCTATTTCGAGGATTCGCTCATGGTCGGTAAAGCAAGTTTCAACTGGATCGACCCGTTGCTGCTGGATCAGCAGCTCACTGAAGAAGAGCGCATGGTGCGTGACAGCGCTTATCAGTTCGCCCAGGACAAGCTGG  >  NC_002947/168233‑168518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑