Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,074,382 G→T 7.4% A158A (GCC→GCA)  gatA ← glutamyl‑tRNA(Gln) amidotransferase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,074,3820GT 7.4% 165.0 / 3.6 54A158A (GCC→GCA) gatAglutamyl‑tRNA(Gln) amidotransferase subunit A
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base T (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GTAGGCGATCATGCCCCAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAGGGTGACCATGCCGGCTTC  >  NC_002947/1074247‑1074528
                                                                                                                                        |                                                                                                                                                  
gTAGGCGATCATGCCCCAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCGCTGCCGTGGCCgcgc                                                                                                                                       <  3:160865/149‑4 (MQ=255)
 tAGGCGATCATGCCCCAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACgg                                                                                                                                                   >  5:197148/1‑136 (MQ=255)
 tAGGCGATCATGCCCCAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACgg                                                                                                                                                   <  6:197148/136‑1 (MQ=255)
 tAGGCGATCATGCCCCAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCCGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCGCTGCCGTGGCCgcgc                                                                                                                                       >  1:59960/1‑145 (MQ=255)
 tAGGCGATCATGCCCCAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCCGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCGCTGCCGTGGCCgcgc                                                                                                                                       <  2:59960/148‑4 (MQ=255)
         cATGCCCCAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCCCCGgacg                                                                                                                              >  4:174297/1‑149 (MQ=255)
              cccAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGAcccg                                                                                                                         >  6:49373/1‑149 (MQ=255)
                cAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCTGGGCATGCCGATACACCCCCCGGTGTACGTTCCCGTTGTTGCGGGCAGCAGACGCGCCGCCACCGCAGCCGCCGAACCAACCGACCAcccgc                                                                                                                        >  1:90902/1‑149 (MQ=255)
                       acacacGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACg                                                                                                                >  3:186442/1‑149 (MQ=255)
                       acacacGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACg                                                                                                                >  2:226264/1‑149 (MQ=255)
                       acacacGA‑CGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGt                                                                                                               >  1:55372/1‑149 (MQ=255)
                           acGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCt                                                                                                            >  5:177053/1‑149 (MQ=255)
                           acGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCt                                                                                                            >  1:34569/1‑149 (MQ=255)
                                      tCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCAc                                                                                                 <  5:49373/149‑1 (MQ=255)
                                              aGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGGCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGGGGTTCCACGGGttct                                                                                          <  7:288191/148‑1 (MQ=255)
                                                gCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCa                                                                                       <  6:177053/149‑1 (MQ=255)
                                                gCCGGTGAGGTTGGTCAGTGCCACT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCa                                                                                       <  1:186324/149‑1 (MQ=255)
                                                 ccGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCAc                                                                                      <  4:186442/149‑1 (MQ=255)
                                                    gTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACcgc                                                                                   >  7:260863/1‑149 (MQ=255)
                                                    gTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACcgc                                                                                   >  4:175507/1‑149 (MQ=255)
                                                          ttGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGACCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGt                                                                              >  8:39139/1‑148 (MQ=255)
                                                                 gTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTg                                                                      <  4:269323/149‑1 (MQ=255)
                                                                    ccGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGAt                                                                   >  6:309526/1‑149 (MQ=255)
                                                                      gct‑ggctgGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTc                                                                 >  4:94039/1‑149 (MQ=255)
                                                                             tggcGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGt                                                                                             >  7:184101/1‑115 (MQ=255)
                                                                             tggcGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGt                                                                                             <  8:184101/115‑1 (MQ=255)
                                                                             tggcGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGc                                                           >  6:261149/1‑149 (MQ=255)
                                                                                 ggATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTg                                                             >  8:257904/1‑143 (MQ=255)
                                                                                 ggATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTg                                                             <  7:257904/143‑1 (MQ=255)
                                                                                  gATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAAc                                                      <  3:94039/149‑1 (MQ=255)
                                                                                   aTCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAAcc                                                     >  5:94395/1‑149 (MQ=255)
                                                                                    tCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCt                                                                       >  1:317833/1‑130 (MQ=255)
                                                                                    tCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCt                                                                       <  2:317833/130‑1 (MQ=255)
                                                                                      gAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCAt                                                  >  8:62098/1‑149 (MQ=255)
                                                                                          cgccgGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGAGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGc                                               >  5:76019/1‑148 (MQ=255)
                                                                                            ccgGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGaa                                            <  1:186780/149‑1 (MQ=255)
                                                                                            ccgGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGaa                                            <  5:261149/149‑1 (MQ=255)
                                                                                              ggTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAAc                                                                                                                 >  2:201053/1‑78 (MQ=255)
                                                                                              ggTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAAc                                                                                                                 <  1:201053/78‑1 (MQ=255)
                                                                                                tgtCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTg                                                             <  4:120592/128‑1 (MQ=255)
                                                                                                tgtCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTg                                                             >  3:120592/1‑128 (MQ=255)
                                                                                                tgtCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCg                                        <  7:62098/149‑1 (MQ=255)
                                                                                                       gCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGt                                 <  3:175507/149‑1 (MQ=255)
                                                                                                         ccGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTg                               >  2:196681/1‑149 (MQ=255)
                                                                                                                     gcagcaGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAgg                   >  7:168180/1‑149 (MQ=255)
                                                                                                                     gcagcaGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAgg                   <  2:34569/149‑1 (MQ=255)
                                                                                                                     gcagcaGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAgg                   >  3:287252/1‑149 (MQ=255)
                                                                                                                       agcagACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCTCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTTGTCTTGCCCAGGGt                 <  1:300430/149‑1 (MQ=255)
                                                                                                                        gcagACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACt                                          >  6:224532/1‑123 (MQ=255)
                                                                                                                        gcagACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACt                                          <  5:224532/123‑1 (MQ=255)
                                                                                                                         cagACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAGGGTGa               <  6:94395/149‑1 (MQ=255)
                                                                                                                           gtggttcgGCCGCTGCCGTGGCCGAACCACCGGACGACCCGCCCGGCACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAGGGTGAc              >  5:205121/7‑148 (MQ=255)
                                                                                                                              gcgcgGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATg                                                 <  2:299221/110‑1 (MQ=255)
                                                                                                                              gcgcgGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATg                                                 >  1:299221/1‑110 (MQ=255)
                                                                                                                                gcgGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAGGGTGACCATGcc        <  5:309526/149‑1 (MQ=255)
                                                                                                                                      aCGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAGGGTGACCATGCCGGCTTc  <  2:55372/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                  
GTAGGCGATCATGCCCCAGCGCGACACACGACCGTACGTCGGCTTGAGGCCGGTGAGGTTGGTCAGTGCCGCT‑GGCTGGCGGATCGAGCCGCCGGTGTCGGTGCCCGTGGTGGCGGGCAGCAGACGCGCGGCCACGGCAGCGGCCGAACCACCGGACGACCCGCCCGGAACGTGCTCGAGGTTCCACGGGTTCTTCACCGCGCCGTAATGGCTGGATTCGTTGGCCGAACCCATGGCGAACTCGTCCATGTTGGTCTTGCCCAGGGTGACCATGCCGGCTTC  >  NC_002947/1074247‑1074528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑