Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,101,800 A→C 7.3% A127A (GCA→GCC) ‡ mlaD → phospholipid ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,101,8000AC 7.3% 169.2 / 4.9 55A127A (GCA→GCC) ‡mlaDphospholipid ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base C (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

GTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGT  >  NC_002947/1101658‑1101946
                                                                                                                                              |                                                                                                                                                   
gTCGGGTGACGTTGGAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatc                                                                                                                                               >  7:268547/1‑149 (MQ=255)
   gggTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTTGCAccatcctt                                                                                                                                            <  4:61195/149‑3 (MQ=255)
               aGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCg                                                                                                                                >  1:93952/1‑149 (MQ=255)
                          tcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCt                                                                                                                     >  2:51390/1‑149 (MQ=255)
                          tcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCt                                                                                                                     >  2:201002/1‑149 (MQ=255)
                            ggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTg                                                                                                                    <  2:267518/148‑1 (MQ=255)
                             gtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTgga                                                                                                                  <  8:252709/149‑1 (MQ=255)
                                 aTAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGAt                                                                                                              >  1:17190/1‑149 (MQ=255)
                                  tAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTgga                                                                                                                  <  1:67359/144‑1 (MQ=255)
                                  tAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTgga                                                                                                                  >  2:67359/1‑144 (MQ=255)
                                       tGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGAtt                                                                                                        >  1:173004/1‑149 (MQ=255)
                                           gaccgacTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGc                                                                                                     >  3:135690/1‑148 (MQ=255)
                                             ccgacTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCgg                                                                                                                               >  7:14215/1‑120 (MQ=255)
                                             ccgacTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCgg                                                                                                                               <  8:14215/120‑1 (MQ=255)
                                                  tCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTgg                                                                                                                         >  5:174950/1‑121 (MQ=255)
                                                  tCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTgg                                                                                                                         <  6:174950/121‑1 (MQ=255)
                                                  tCCACTGCCTCGATCCTGACCGCCGGTTTGATTGGTGAGAAGTAGATGGGGATCAGCTTTGGCGGTGAAGACGAATTGCTCAAGGATGGTGCAAGCATCCATGACACACATTCGGCACTGGTGCTGGATGATGTGATTGGCAAGTTCCt                                                                                             <  4:273259/149‑1 (MQ=255)
                                                    cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTg                                                                                            <  2:173004/148‑1 (MQ=255)
                                                     aCTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGc                                                                                           >  3:147502/1‑148 (MQ=255)
                                                          cTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAAct                                                                                     <  1:201002/149‑1 (MQ=255)
                                                             gATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTg                                                                                  >  6:1271/1‑149 (MQ=255)
                                                               tCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGtt                                                                                >  3:40360/1‑149 (MQ=255)
                                                                ccTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGtt                                                                                <  4:147502/148‑1 (MQ=255)
                                                                 cTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTg                                                                               <  7:226868/148‑1 (MQ=255)
                                                                     ccgccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAg                                                                          <  7:172130/149‑1 (MQ=255)
                                                                       gccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGa                                                                        <  2:93952/149‑1 (MQ=255)
                                                                       gccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTCTTGGCAAgg                                                                         >  1:43144/1‑148 (MQ=255)
                                                                        ccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGCTCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAggca                                                                       >  4:212732/1‑147 (MQ=255)
                                                                                   ggTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaag‑aa                                                             <  3:166059/147‑1 (MQ=255)
                                                                                   ggTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaag‑aa                                                             <  2:17190/147‑1 (MQ=255)
                                                                                      gagaAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAgcgc                                                         >  7:252352/1‑148 (MQ=255)
                                                                                       agaAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCaa                                                       >  1:156859/1‑149 (MQ=255)
                                                                                        gaAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCaa                                                       <  6:37507/148‑1 (MQ=255)
                                                                                            tACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGc                                                  >  2:164303/1‑149 (MQ=255)
                                                                                                    cATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAg                                          <  8:140270/149‑1 (MQ=255)
                                                                                                          cGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGCAAGCGCAACCGGCTAATTAAGGAGTa                                     <  1:164303/149‑1 (MQ=255)
                                                                                                          cGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTAc                                    <  1:51390/149‑1 (MQ=255)
                                                                                                            ttGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTAccc                                  <  4:135690/149‑1 (MQ=255)
                                                                                                                       gACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCaa                                                                                                   <  4:276478/74‑1 (MQ=255)
                                                                                                                       gACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCaa                                                                                                   >  3:276478/1‑74 (MQ=255)
                                                                                                                          gAAGTGCTCAAGGATGGTGCAACCATCCATGCCACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCt                    >  4:120399/1‑149 (MQ=255)
                                                                                                                          gAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCt                    >  4:125070/1‑149 (MQ=255)
                                                                                                                                cTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGGGACg              >  5:325474/1‑149 (MQ=255)
                                                                                                                                  catggATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCg                         <  4:70116/133‑1 (MQ=255)
                                                                                                                                  catggATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCg                         >  3:70116/4‑136 (MQ=255)
                                                                                                                                   atggATGGTTGCACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTgg           >  3:229945/3‑149 (MQ=255)
                                                                                                                                   aaGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaag‑aa                                                             <  1:1213/99‑1 (MQ=255)
                                                                                                                                   aaGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaag‑aa                                                             >  2:1213/1‑99 (MQ=255)
                                                                                                                                   aaGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaag‑aa                                                             >  6:176335/1‑99 (MQ=255)
                                                                                                                                   aaGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaag‑aa                                                             <  5:176335/99‑1 (MQ=255)
                                                                                                                                      gATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCct        >  1:292361/1‑149 (MQ=255)
                                                                                                                                         ggTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCctgc      <  8:252352/148‑1 (MQ=255)
                                                                                                                                          gTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCctgctg    <  2:292361/149‑1 (MQ=255)
                                                                                                                                          gTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCctgctg    <  4:40360/149‑1 (MQ=255)
                                                                                                                                            gCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGt  >  7:140227/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAG‑AAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGT  >  NC_002947/1101658‑1101946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑