Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,102,435 G→C 5.3% R174P (CGT→CCT) ‡ ttg2D → toluene tolerance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,102,4350GC 5.3% 251.1 / 3.2 76R174P (CGT→CCT) ‡ttg2Dtoluene tolerance protein
Reads supporting (aligned to +/- strand):  ref base G (37/35);  new base C (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

ATGACGGCAAGCGCGCCAGTGTCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGC  >  NC_002947/1102288‑1102579
                                                                                                                                                   |                                                                                                                                                
aTGACGGCAAGCGCGCCAGTGTCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGt                                                                                                                                                 >  4:206664/1‑149 (MQ=255)
   aCGGCAAGCGCGCCAGTGTCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGAc                                                                                                                                              <  3:106001/149‑1 (MQ=255)
     ggCAAGCGCGCCAGTGTCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCa                                                                                                                                            <  6:308590/149‑1 (MQ=255)
      gCAAGCGCGCCAGTGTCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAg                                                                                                                                           >  7:145/1‑149 (MQ=255)
          gcgcgcCAGTGTCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCg                                                                                                                                       >  5:125119/1‑149 (MQ=255)
              gcCAGTGTCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGTCGa                                                                                                                                   >  3:166515/1‑149 (MQ=255)
                  gtgtCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGc                                                                                                                                >  4:71456/1‑148 (MQ=255)
                       ggCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCGAACTGGTCACGGAACAGCTTGCCGATgttgatg                                                                                                                           <  1:173891/148‑7 (MQ=37)
                         cATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAgc                                                                                                                        <  6:125119/149‑1 (MQ=255)
                            gAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGAt                                                                                                                                  >  8:282295/1‑136 (MQ=255)
                            gAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCCCCGAt                                                                                                                                  <  7:282295/136‑1 (MQ=255)
                               ggTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACg                                                                                                                  >  2:308559/1‑149 (MQ=255)
                               ggTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACg                                                                                                                  <  4:53989/149‑1 (MQ=255)
                                    ccGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCACAACGGCAAt                                                                                                             >  7:96255/1‑149 (MQ=255)
                                     cGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCa                                                                                                                                            <  2:187379/117‑1 (MQ=255)
                                     cGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCa                                                                                                                                            >  1:187379/1‑117 (MQ=255)
                                     cGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATg                                                                                                            <  7:249789/149‑1 (MQ=255)
                                      ggCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGa                                                                                                           >  5:59212/1‑149 (MQ=255)
                                       gCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATGGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGAc                                                                                                          <  1:308559/149‑1 (MQ=255)
                                         aacaacGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGTCATGCAGCGCAACGGCAATGACCt                                                                                                        >  3:11305/1‑149 (MQ=255)
                                         aacaacGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCt                                                                                                        <  3:71456/149‑1 (MQ=255)
                                                          ccccGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAAt                                                                                                             >  2:27529/1‑127 (MQ=255)
                                                          ccccGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACg                                                                                       >  5:202169/1‑149 (MQ=255)
                                                          ccccGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACg                                                                                       >  7:58835/1‑149 (MQ=255)
                                                          ccccGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCACTTCGCCGATGCCATGCAGCGCAACGGCAAt                                                                                                             <  1:27529/127‑1 (MQ=255)
                                                           cccGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACgg                                                                                      >  5:37854/1‑149 (MQ=255)
                                                           cccGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACgg                                                                                      >  2:43672/1‑149 (MQ=255)
                                                               tGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGTCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTgg                                                                                  <  4:11305/149‑1 (MQ=255)
                                                                  aGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGcc                                                                               >  2:43424/1‑149 (MQ=255)
                                                                   gTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGt                                                                                                                                                 >  2:147615/1‑82 (MQ=255)
                                                                    tACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCgg                                                                             >  1:5314/1‑149 (MQ=255)
                                                                      cacCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGc                                                                            <  8:256410/148‑1 (MQ=255)
                                                                         ccTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAg                                                                        >  6:20891/1‑149 (MQ=255)
                                                                         ccTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGCCTGGGCCGGCGAAg                                                                        >  3:248552/1‑149 (MQ=255)
                                                                          cTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAAGGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGTCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGt                                                                       <  4:166515/149‑1 (MQ=255)
                                                                             gagaACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTggc                                                                    >  4:180700/1‑149 (MQ=255)
                                                                                 aCATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTggccaag                                                                <  3:206664/149‑1 (MQ=255)
                                                                                  cATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTggccaagg                                                               <  2:5314/149‑1 (MQ=255)
                                                                                  cATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTggc                                                                    <  7:260434/144‑1 (MQ=255)
                                                                                  cATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTggc                                                                    >  8:260434/1‑144 (MQ=255)
                                                                                                 gAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGcc                                                 >  7:140937/1‑148 (MQ=255)
                                                                                                   aGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGa                                               <  6:37854/148‑1 (MQ=255)
                                                                                                     gTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACaa                                            <  1:43424/149‑1 (MQ=255)
                                                                                                     gTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCGGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACaa                                            <  8:145/149‑1 (MQ=255)
                                                                                                         gTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACaaaacg                                        >  8:194817/1‑145 (MQ=255)
                                                                                                           aaCGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGAc                                                                                                    >  4:97037/1‑87 (MQ=255)
                                                                                                           aaCGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGAc                                                                                                    <  3:97037/87‑1 (MQ=255)
                                                                                                            aCGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCa                                     <  7:147614/149‑1 (MQ=255)
                                                                                                            aCGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCACAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCa                                     <  8:96255/149‑1 (MQ=255)
                                                                                                              gTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGgccaaggccaag                                                          >  4:244344/1‑126 (MQ=255)
                                                                                                              gTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGgccaaggccaag                                                          <  3:244344/126‑1 (MQ=255)
                                                                                                               tGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCaga                                   <  1:95259/148‑1 (MQ=255)
                                                                                                               tGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCa                                     >  2:95259/1‑146 (MQ=255)
                                                                                                                 aTCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAgaga                                 <  1:43672/148‑1 (MQ=255)
                                                                                                                    gTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGagaaga                              <  6:202169/148‑1 (MQ=255)
                                                                                                                     tCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGc                            <  8:58835/149‑1 (MQ=255)
                                                                                                                     tCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGc                            >  4:109645/1‑149 (MQ=255)
                                                                                                                        actgCATCAACATCGGCAAGCTTTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCTGACAACTCGCCAGAGAAGAGCGTc                         <  7:194817/146‑1 (MQ=255)
                                                                                                                         cGGCATCAACATCGGCAAGCTGTTCCGTGGCCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTc                         >  1:136531/1‑148 (MQ=255)
                                                                                                                            cATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAAt                     >  6:269624/1‑149 (MQ=255)
                                                                                                                             aTCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGagaaga                              >  8:240305/1‑139 (MQ=255)
                                                                                                                             aTCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGagaaga                              <  7:240305/139‑1 (MQ=255)
                                                                                                                             aTCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAAt                     >  3:297082/1‑148 (MQ=255)
                                                                                                                               tggcATCGGCGAACTGGTCACGGAACAGCTTGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGa                                               >  6:88050/4‑120 (MQ=25)
                                                                                                                               tggcATCGGCGAACTGGTCACGGAACAGCTTGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGa                                               <  5:88050/117‑1 (MQ=25)
                                                                                                                               cAACATCGGCAAGGTGTTCCGTGACCATTGCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGTCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGAc                                              <  6:173537/121‑1 (MQ=255)
                                                                                                                               cAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTTCCGAc                                              >  5:173537/1‑121 (MQ=255)
                                                                                                                                taCATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGGCAAGCAGGCTGCCGAGATCTCGCCAGAGAAGAGCGTCAAAtgagt                 <  8:260598/148‑1 (MQ=255)
                                                                                                                                ggcATCGGCGAACTGGTCACGGAACAGCTTGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAAtgagt                 >  3:128283/3‑149 (MQ=38)
                                                                                                                                    tCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAgg             >  1:111574/1‑149 (MQ=255)
                                                                                                                                         aaGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCt        <  5:20891/149‑1 (MQ=255)
                                                                                                                                               ttCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAAt                     <  8:162601/130‑1 (MQ=255)
                                                                                                                                               ttCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAAt                     >  7:162601/1‑130 (MQ=255)
                                                                                                                                               ttCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGc  <  8:140937/149‑1 (MQ=255)
                                                                                                                                                 ccGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTc                         <  4:147513/124‑1 (MQ=255)
                                                                                                                                                 ccGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTc                         >  3:147513/1‑124 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ATGACGGCAAGCGCGCCAGTGTCGGCATGAAGGTTACCGGCAACAACGGTGCCGTGTACCCCGTGCAGTACACCCTGGAGAACATTGGCGGCGAGTGGAAGGTGCGTAACGTGATCGTCAACGGCATCAACATCGGCAAGCTGTTCCGTGACCAGTTCGCCGATGCCATGCAGCGCAACGGCAATGACCTGGACAAGACCATCGACGGCTGGGCCGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGC  >  NC_002947/1102288‑1102579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑