Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,120,470 T→A 14.4% intergenic (‑212/‑90) pepA ← / → PP_0982 cytosol aminopeptidase/YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,120,4700TA14.4% 132.8 / 10.5 54intergenic (‑212/‑90)pepA/PP_0982cytosol aminopeptidase/YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base T (24/22);  new base A (4/4);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

CCGCGTCGTCGCGCACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGC  >  NC_002947/1120323‑1120616
                                                                                                                                                    |                                                                                                                                                  
ccGCGTCGGCGCGCACCAGCGGAATAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCttt                                                                                                                                                   <  8:128824/149‑1 (MQ=255)
    gtcgtcGCTCACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGGCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCg                                                                                                                                               <  3:108845/149‑1 (MQ=255)
           cgcACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCAAAGGCGAAcctcc                                                                                                                                        <  4:255331/149‑4 (MQ=255)
             cACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCAt                                                                                                                                      <  2:105673/149‑1 (MQ=255)
                   cGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCt                                                                                                                                <  7:127213/149‑1 (MQ=255)
                       aaaGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGa                                                                                                                            <  3:29808/149‑1 (MQ=255)
                        aaGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGAt                                                                                                                           <  7:181895/149‑1 (MQ=255)
                           cTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGt                                                                                                                         >  8:287170/1‑148 (MQ=255)
                                    tGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGAt                                                                                                                           >  4:310404/1‑137 (MQ=255)
                                    tGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGAt                                                                                                                           <  3:310404/137‑1 (MQ=255)
                                       gCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGt                                                                                                            >  8:100426/1‑149 (MQ=255)
                                           gtgGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGt                                                                                                                                  >  7:112490/1‑123 (MQ=255)
                                           gtgGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCAAAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGt                                                                                                                                  <  8:112490/123‑1 (MQ=255)
                                              gCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTa                                                                                                                        >  8:193827/1‑130 (MQ=255)
                                              gCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTa                                                                                                                        <  7:193827/130‑1 (MQ=255)
                                                  gCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCAtt                                                                                                 <  5:167093/149‑1 (MQ=255)
                                                    gTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGc                                                                                               <  1:197907/149‑1 (MQ=255)
                                                          aCAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTTACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGTTT‑CGACTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCa                                                                                         <  3:258941/149‑1 (MQ=255)
                                                                 cGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGcc                                                                                  >  2:66047/1‑149 (MQ=255)
                                                                         gcCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGc                                                                                          <  4:13181/133‑1 (MQ=255)
                                                                         gcCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGc                                                                                          >  3:13181/1‑133 (MQ=255)
                                                                           cTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCAAAGGCGAAcctcc                                                                                                                                        >  6:271314/1‑82 (MQ=255)
                                                                           cTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCAAAGGCGAAcctcc                                                                                                                                        <  5:271314/85‑4 (MQ=255)
                                                                                       ccAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTAAGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTg                                                             >  1:245586/1‑149 (MQ=255)
                                                                                         atttGACTGGGGCGGTAAATCCATGTATAATGGGCGAAGTTTACATGGAGGTT‑GGCCTTGGGCTAACGGGAATTGGTCTTGTATGTATTATGTCTTTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGtt                                                          <  1:66047/146‑1 (MQ=255)
                                                                                             gACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGAt                                                      >  5:93884/1‑149 (MQ=255)
                                                                                              aCAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATc                                                     >  3:181797/1‑149 (MQ=255)
                                                                                                  gggCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGc                                                                                          <  3:54437/108‑1 (MQ=255)
                                                                                                  gggCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGc                                                                                          >  4:54437/1‑108 (MQ=255)
                                                                                                       gTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGt                                            >  3:171436/1‑149 (MQ=255)
                                                                                                        tCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGt                                            >  7:185141/1‑148 (MQ=255)
                                                                                                        tCAATAACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTATGGCGAACCGGAATTGGTCTTGGATGTACTAATTCATTGTTTGGCGCCATTGCATGGCAGTCAGCACTGACAACCCAGGAGTGTCTGGTTTGAGCGTCTGTCGt                                            >  2:91045/1‑148 (MQ=255)
                                                                                                          aaTCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCTAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTAt                                         <  8:17010/149‑1 (MQ=255)
                                                                                                               caGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATgg                                                                                           <  1:17006/94‑1 (MQ=255)
                                                                                                               caGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATgg                                                                                           >  2:17006/1‑94 (MQ=255)
                                                                                                                   aTAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCcgc                                <  4:181797/149‑1 (MQ=255)
                                                                                                                     aaTGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCcgc                                >  3:263696/1‑147 (MQ=255)
                                                                                                                      aTGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGa                              <  8:185141/148‑1 (MQ=255)
                                                                                                                              aCTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTgg                      >  8:250646/1‑148 (MQ=255)
                                                                                                                                 ttACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCAc                  >  7:134705/1‑149 (MQ=255)
                                                                                                                                     aTGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGTGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCtt               >  3:47314/1‑148 (MQ=255)
                                                                                                                                     aTGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCtt               >  7:27214/1‑148 (MQ=255)
                                                                                                                                       ggAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCcgc                                >  2:135882/1‑129 (MQ=255)
                                                                                                                                       ggAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCcgc                                <  1:135882/129‑1 (MQ=255)
                                                                                                                                       ggAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAg            >  1:107744/1‑149 (MQ=255)
                                                                                                                                        gAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAg            <  2:237890/148‑1 (MQ=255)
                                                                                                                                         cggTT‑CGCCAAAGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAgg                                                                     >  2:212350/2‑90 (MQ=255)
                                                                                                                                         cggTT‑CGCCAAAGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAgg                                                                     >  2:207885/2‑90 (MQ=255)
                                                                                                                                         cggTT‑CGCCAAAGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAgg                                                                     <  1:212350/89‑1 (MQ=255)
                                                                                                                                         cggTT‑CGCCAAAGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAgg                                                                     <  1:207885/89‑1 (MQ=255)
                                                                                                                                               cGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGt      <  4:263696/148‑1 (MQ=255)
                                                                                                                                                gCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTc     <  4:171436/148‑1 (MQ=255)
                                                                                                                                                 ccTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAg   >  8:85875/1‑149 (MQ=255)
                                                                                                                                                 ccTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAg   >  4:139763/1‑149 (MQ=255)
                                                                                                                                                  cTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGTGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAgc  >  6:306525/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCGCGTCGTCGCGCACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTT‑CGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGC  >  NC_002947/1120323‑1120616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑