Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,184,152	A→T	7.0%	V78E (GTA→GAA)	mltF ←	membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,184,152	0	A	T	7.0%	173.9 / 2.9	57	V78E (GTA→GAA)	mltF	membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base T (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

ACCTCCAGATAGGGATGGGAGTATCTCACCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAGGGTGCTGG  >  NC_002947/1184005‑1184293                                                                                                                                                    |                                                                                                                                                aCCTCCAGATAGGGATGGGAGTATCTCACCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTAc                                                                                                                                                >  6:262401/1‑149 (MQ=255)     ccAGATAGGGATGGGAGTATCTCACCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTAGGCGGTCTCGATCTGCAGCTTTACGcc                                                                                                                                             >  8:267640/1‑148 (MQ=255)          tAGGTATGGGAGTATCTCACCTGGTTGTTGCGCCGCTCACTGCTGACCAGGCGGGCTGCAGCCAGTACCGGTCCGGAGGGCTTTCCGAGTGCGTCTTACAGCTCGTCCTGGTTGTCGGCGGTCTCTATCTGCAGCTTTACGCCGA‑GAtc                                                                                                                                      <  6:169066/149‑1 (MQ=255)                   gAGTATCTCACCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGa                                                                                                                              >  8:84580/1‑148 (MQ=255)                    aGTATCTCACCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGaaa                                                                                                                            >  8:31601/1‑149 (MQ=255)                          tcACCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGGTg                                                                                                                       >  2:301737/1‑148 (MQ=255)                          tcACCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCATTTCGCCGACGATC‑TCGGCGTAAAGCTgc                                                                                                                      >  4:47320/1‑148 (MQ=255)                            aCCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTg                                                                                                                       <  7:84580/146‑1 (MQ=255)                            aCCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCATTTCGCCGACGATC‑TCGGCGTAAAGCTgcag                                                                                                                    <  3:47320/149‑4 (MQ=38)                            aCCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCATTTCGCCGACGATC‑TCGGCGTAAAGCTgc                                                                                                                      <  2:55713/147‑2 (MQ=255)                              cTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGTCAGCCAGTACCGGGACGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGATTTACGCCGA‑GATCGTCGGCGAAATGCTGGAc                                                                                                                    >  2:203900/1‑147 (MQ=255)                              cTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGaaa                                                                                                                            <  4:134000/139‑1 (MQ=255)                              cTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGaaa                                                                                                                            >  3:134000/1‑139 (MQ=255)                              cTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGAc                                                                                                                    >  1:110513/1‑147 (MQ=255)                                 ggTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGGTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGtt                                                                                                               <  8:257024/149‑1 (MQ=255)                                                aCTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACTGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑gg                                                                                               >  1:89708/1‑149 (MQ=255)                                                aCTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTg                                                                                                                       <  6:90200/126‑1 (MQ=255)                                                aCTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTg                                                                                                                       >  5:90200/1‑126 (MQ=255)                                                aCTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑g                                                                                                <  7:31601/148‑1 (MQ=255)                                                  tgctgACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTg                                                                                                                       >  3:305543/1‑124 (MQ=255)                                                  tgctgACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTg                                                                                                                       <  4:305543/124‑1 (MQ=255)                                                       aCCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGcc                                                                                        >  3:252493/1‑149 (MQ=255)                                                       aCCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGc                                                                                         <  5:286258/148‑1 (MQ=255)                                                                   gcAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTg                                                                            <  8:161746/149‑1 (MQ=255)                                                                     aGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCAGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTgg                                                                           <  8:150811/148‑1 (MQ=255)                                                                     aGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCAGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTgg                                                                           >  7:150811/1‑148 (MQ=255)                                                                      gCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGaa                                                                         <  7:28952/149‑1 (MQ=255)                                                                           tACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCg                                                                                          >  4:188666/1‑127 (MQ=255)                                                                           tACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCg                                                                                          <  3:188666/127‑1 (MQ=255)                                                                                    ggAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGc                                                           <  5:262401/149‑1 (MQ=255)                                                                                          cTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGc                                                     <  2:110513/149‑1 (MQ=255)                                                                                           ttGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCg                                                    >  3:138760/1‑149 (MQ=255)                                                                                              ccGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCgtgt                                                 <  4:252493/149‑1 (MQ=255)                                                                                              ccGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCgtgt                                                 <  1:203900/149‑1 (MQ=255)                                                                                                gAGGGCGTCGTACTGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGa                                               <  2:89708/149‑1 (MQ=255)                                                                                                  gggCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATc                                             <  8:40531/149‑1 (MQ=255)                                                                                                        cgtACAGTTCGTCTAGGTTGTAGGCTGTCTCGATCTGCAGCTTTAGGCCTA‑GATCGTCGGCGAAATGCTTTTCCTGTTCGTACTCAAAGCC‑TTTTTCGCCGTTACGTTCCTGTAACTATGTTGCCGGGCTGTTGCGTGTGATCActcg                                        <  4:174973/148‑1 (MQ=255)                                                                                                                  gTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCAt                             >  3:237291/1‑149 (MQ=255)                                                                                                                       ggTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCAtcctcc                        >  8:206642/1‑149 (MQ=255)                                                                                                                        gTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATcctcct                       >  3:98096/1‑149 (MQ=255)                                                                                                                           gTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCa                              >  1:235437/1‑139 (MQ=255)                                                                                                                           gTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCa                              <  2:235437/139‑1 (MQ=255)                                                                                                                               gcggTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTc                                                                                           <  8:215009/74‑1 (MQ=255)                                                                                                                               gcggTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTc                                                                                           >  7:215009/1‑74 (MQ=255)                                                                                                                                  gTCTCGATCTTCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGATCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTgg                                                                           <  1:145745/87‑1 (MQ=255)                                                                                                                                  gTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGATCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTgg                                                                           >  2:145745/1‑87 (MQ=255)                                                                                                                                   tctcGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTc             >  2:79899/1‑148 (MQ=255)                                                                                                                                    ctcGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTc             >  3:105770/1‑147 (MQ=255)                                                                                                                                     tcGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAg          >  7:10271/1‑149 (MQ=255)                                                                                                                                       gATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCTTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAgg         <  7:267640/148‑1 (MQ=255)                                                                                                                                       gATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAggg        >  4:263508/1‑149 (MQ=255)                                                                                                                                       gATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAggg        >  4:259130/1‑149 (MQ=255)                                                                                                                                       gATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAgg         <  7:206642/148‑1 (MQ=255)                                                                                                                                             cAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAggg        <  8:10271/143‑1 (MQ=255)                                                                                                                                             cAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAggg        <  4:98096/143‑1 (MQ=255)                                                                                                                                             cAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAGGGTGCTgg  >  3:157399/1‑149 (MQ=255)                                                                                                                                              aGCATTTCGCCGACGATC‑TCGGCGTAAAGCTGGACCAGTTCGTACTCAAAGCCTAGTTTCGCCGTTACGGTCATGGAAGTAGGTTGCCGGCCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAGGGTGCTgg  >  1:241875/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                ACCTCCAGATAGGGATGGGAGTATCTCACCTGGGTTTTGCGCCGCTCACTGCTGACCAGGCCGGCGGCAGCCAGTACCGGGCCGGAGGGCTTGCCGAGGGCGTCGTACAGCTCGTCCAGGTTGTCGGCGGTCTCGATCTGCAGCTTTACGCCGA‑GATCGTCGGCGAAATGCTGGACCAGTTCGTACTCAAAGCC‑GGTTTCGCCGTTACGGTCCTGGAAGTAGGTTGCCGGGCTGTTGCGTGTGATCACGCGCAGCACGCCATCCTCCTTCACGCGCTCGAGGGTGCTGG  >  NC_002947/1184005‑1184293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑