Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,207,624 T→C 5.3% A459A (GCA→GCG)  PP_1058 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,207,6240TC 5.3% 221.2 / 2.7 75A459A (GCA→GCG) PP_1058hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/34);  new base C (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

GTTGATGTCGAAGGGCTGGTTGGTGCTGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTG  >  NC_002947/1207480‑1207765
                                                                                                                                                |                                                                                                                                             
gTTGATGTCGAAGGGCTGGTTGGTGCTGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCgg                                                                                                                                           >  7:244398/1‑149 (MQ=255)
    aTGTCGAAGGGCTGGTTGGTGCTGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCaa                                                                                                                                        <  8:244398/148‑1 (MQ=255)
    aTGTCGAAGGGCTGGTTGGTGCTGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCAGCTCGCCGATCAGGCCCAGAGGTGCGGCaa                                                                                                                                        >  8:267647/1‑148 (MQ=255)
         gAAGGGCTGGTTGGTGCTGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCg                                                                                                                                  >  3:2689/1‑149 (MQ=255)
              gCTGGTTGGTGCTGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCACGCCGATCTGTGCGGCAAACTCCGGGTCg                                                                                                                             <  1:179563/149‑1 (MQ=255)
                  gttggtGCTGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGAGTTCGAACAAGGTGATGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGGGGCAAAGTCCGGGTCGGCaa                                                                                                                         <  1:166340/149‑1 (MQ=255)
                          tGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGttttt                                                                                                                  >  4:141766/1‑148 (MQ=255)
                            tCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAg                                                                                                               >  3:283034/1‑149 (MQ=255)
                              ggTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAgg                                                                                                              >  2:91454/1‑148 (MQ=255)
                               gTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGttttt                                                                                                                  <  2:73631/143‑1 (MQ=255)
                               gTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGttttt                                                                                                                  >  1:73631/1‑143 (MQ=255)
                                    cACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACt                                                                                                          <  1:172171/146‑1 (MQ=255)
                                       ccGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTaa                                                                                                    >  1:119851/1‑149 (MQ=255)
                                        cGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAAc                                                                                                   >  2:38734/1‑149 (MQ=255)
                                          gACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCt                                                                                                 <  1:181418/149‑1 (MQ=255)
                                             ccGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGc                                                                                               <  1:173357/148‑1 (MQ=255)
                                             ccGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGc                                                                                               >  2:173357/1‑148 (MQ=255)
                                               gcgAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTAc                                                                                                           >  1:200898/1‑134 (MQ=255)
                                               gcgAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTAc                                                                                                           <  2:200898/134‑1 (MQ=255)
                                               gcgAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGt                                                                                            >  3:205756/1‑149 (MQ=255)
                                                    ccATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAg                                                                                       <  1:91454/149‑1 (MQ=255)
                                                    ccATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAg                                                                                       >  7:11457/1‑149 (MQ=255)
                                                    ccATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAg                                                                                       <  4:2689/149‑1 (MQ=255)
                                                         ggcggTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAg                                                                                                               <  7:291241/120‑1 (MQ=255)
                                                         ggcggTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAg                                                                                                               >  8:291241/1‑120 (MQ=255)
                                                         ggcggTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCg                                                                                   <  4:283034/148‑1 (MQ=255)
                                                            ggTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGc                                                                                 >  2:203531/1‑147 (MQ=255)
                                                            ggTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGc                                                                                 <  1:203531/147‑1 (MQ=255)
                                                                  ttCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCgg                                                                                  >  2:275751/1‑140 (MQ=255)
                                                                  ttCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCgg                                                                                  <  1:275751/140‑1 (MQ=255)
                                                                  ttCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAgg                                                                         >  5:215969/1‑149 (MQ=255)
                                                                        cAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGACCTGCAAg                                                                          >  6:129162/1‑142 (MQ=255)
                                                                        cAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGACCTGCAAg                                                                          <  5:129162/142‑1 (MQ=255)
                                                                         aaGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGc                                                                                                       >  8:136660/1‑112 (MQ=255)
                                                                         aaGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGc                                                                                                       <  7:136660/112‑1 (MQ=255)
                                                                         aaGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggt                                                                  <  6:35116/149‑1 (MQ=255)
                                                                          aGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggtg                                                                 <  1:130677/149‑1 (MQ=255)
                                                                           ggTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggtgg                                                                >  1:183009/1‑149 (MQ=255)
                                                                             tGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCt                                                              >  6:241727/1‑149 (MQ=255)
                                                                               aaGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggtgg                                                                >  6:279441/1‑145 (MQ=255)
                                                                               aaGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggtgg                                                                <  5:279441/145‑1 (MQ=255)
                                                                                 gCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGa                                                            >  1:304959/1‑147 (MQ=255)
                                                                                 gCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACa                                                          <  1:38734/149‑1 (MQ=255)
                                                                                  cTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTc                                                                                                                              <  2:306426/80‑1 (MQ=255)
                                                                                  cTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTc                                                                                                                              >  1:306426/1‑80 (MQ=255)
                                                                                    gTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCAAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACAGGTGGCTGACAGGt                                                       >  8:231495/1‑149 (MQ=255)
                                                                                        cTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAg                                                   >  6:277955/1‑149 (MQ=255)
                                                                                        cTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGCGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAAcc                                                                                                  >  7:216908/1‑102 (MQ=255)
                                                                                        cTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGCGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAAcc                                                                                                  <  8:216908/102‑1 (MQ=255)
                                                                                        cTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGCGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAAcc                                                                                                  >  7:212817/1‑102 (MQ=255)
                                                                                        cTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGCGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAAcc                                                                                                  <  8:212817/102‑1 (MQ=255)
                                                                                         tCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAgg                                                  >  2:177901/1‑149 (MQ=255)
                                                                                           aCCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAgg                                                  <  3:141766/147‑1 (MQ=255)
                                                                                           aCCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCg                                                >  2:284600/1‑149 (MQ=255)
                                                                                                gTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTc                                             >  6:114554/1‑147 (MQ=255)
                                                                                                gTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCg                                            <  6:215969/148‑1 (MQ=255)
                                                                                                 tCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAg                                          <  2:304959/149‑1 (MQ=255)
                                                                                                 tCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAg                                          <  5:241727/149‑1 (MQ=255)
                                                                                                  cGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAgg                                         >  4:196903/1‑149 (MQ=255)
                                                                                                   ggtggtCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAgg                                         <  1:284600/148‑1 (MQ=255)
                                                                                                    gtggtCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTc                                       >  6:57293/1‑149 (MQ=255)
                                                                                                    gtggtCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTc                                       >  5:253802/1‑149 (MQ=255)
                                                                                                     tggtCTTGGCAGTGAGGAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGGAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGTCAGGTCGAGGCGGTCGAGGTCg                                      <  7:231495/149‑1 (MQ=255)
                                                                                                     tggtCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCg                                      <  2:119851/149‑1 (MQ=255)
                                                                                                     tggtCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCg                                      <  7:267647/149‑1 (MQ=255)
                                                                                                             gCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGc                                                                                         <  8:265623/90‑1 (MQ=255)
                                                                                                             gCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGc                                                                                         >  7:265623/1‑90 (MQ=255)
                                                                                                                 tGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGa                          <  2:183009/149‑1 (MQ=255)
                                                                                                                 tGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGa                          >  6:306057/1‑149 (MQ=255)
                                                                                                                 tGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGa                          <  5:114554/149‑1 (MQ=255)
                                                                                                                   agcagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGAtt                        >  1:214482/1‑149 (MQ=255)
                                                                                                                                 gATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTc                                             >  3:262364/1‑114 (MQ=255)
                                                                                                                                 gATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTc                                             <  4:262364/114‑1 (MQ=255)
                                                                                                                                 gATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACa          <  6:253802/149‑1 (MQ=255)
                                                                                                                                         cGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTg  >  5:267146/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GTTGATGTCGAAGGGCTGGTTGGTGCTGTCGGTCTGCACCCGGACCCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTG  >  NC_002947/1207480‑1207765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑