Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,254,439 C→A 6.4% L354L (CTG→CTT)  metG ← methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,254,4390CA 6.4% 199.6 / 3.9 63L354L (CTG→CTT) metGmethionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base A (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

GTTGCCCTTGTGGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGG  >  NC_002947/1254292‑1254584
                                                                                                                                                    |                                                                                                                                                 
gTTGCCCTTGTGGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCa                                                                                                                                                  <  1:268814/149‑1 (MQ=255)
    cccTTGTGGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTa                                                                                                                                              >  7:214965/1‑149 (MQ=255)
     ccTTGTGGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTAt                                                                                                                                             >  3:10479/1‑149 (MQ=255)
      cTTGTGGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGAAGGTAtt                                                                                                                                            >  2:17469/1‑149 (MQ=255)
      cTTGTGGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGAAGGTAtt                                                                                                                                            >  2:12503/1‑149 (MQ=255)
         gtgGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTc                                                                                                                                           <  6:113401/147‑1 (MQ=255)
         gtgGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCg                                                                                                                                         >  2:237378/1‑149 (MQ=255)
           ggATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCgga                                                                                                                                       >  8:71038/1‑148 (MQ=255)
           ggATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCgg                                                                                                                                        <  3:121306/148‑1 (MQ=255)
             aTGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAGAAAATCCAGGTTCAGGGACAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAAAGCAGGTATTCCGGCt                                                                                                                                      >  1:256342/1‑149 (MQ=255)
                 aaCCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCTACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAgg                                                                                                                                 <  1:237378/149‑1 (MQ=255)
                                        ttGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGAAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCt                                                                                                           <  1:17469/148‑1 (MQ=255)
                                        ttGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGAAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCt                                                                                                           <  1:12503/148‑1 (MQ=255)
                                          gacgacCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTtga                                                                                                        >  5:281677/1‑149 (MQ=255)
                                            cgacCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTtgatg                                                                                                      <  5:140890/149‑1 (MQ=255)
                                               ccTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAg                                                                                                   <  6:102068/149‑1 (MQ=255)
                                               ccTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAg                                                                                                   <  4:10479/149‑1 (MQ=255)
                                                    ccGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTg                                                                                                >  8:180491/1‑147 (MQ=255)
                                                     cGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCcg                                                                                             >  6:242053/1‑149 (MQ=255)
                                                     cGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGAGCCTTGATGAAGGTGCcg                                                                                             >  8:15017/1‑149 (MQ=255)
                                                      gACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGTCACAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGAAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCcgc                                                                                            <  8:214965/149‑1 (MQ=255)
                                                              cGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTg                                                                                    <  8:141027/149‑1 (MQ=255)
                                                                gccatggccTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGa                                                                                  <  5:170337/142‑1 (MQ=255)
                                                                 aGTTGACCATCTGCACGAAG‑TCTACCAGGGTCACGTCCAGGTCGTCGACGCCACGGCCCAGCTTCGCCGCATAGTAATACCGCAGGTATTCCGGCGGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGAc                                                                                 <  4:75525/149‑1 (MQ=255)
                                                                     gACCTTCTGCACGAAG‑TCTTCCAGGTTTAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCt                                                                             >  7:12046/1‑149 (MQ=255)
                                                                      aCCTTCTGCACGAAG‑TCTTCCAGGTGCCGGTCCAGGGCCTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCCCGTGGTCCAGGTAGGTCCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCtt                                                                            <  4:90721/149‑1 (MQ=255)
                                                                       ccTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTg                                                                           >  4:135458/1‑149 (MQ=255)
                                                                         ttCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGgc                                                                         >  1:143627/1‑149 (MQ=255)
                                                                         ttCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGgc                                                                         >  1:297559/1‑149 (MQ=255)
                                                                           cTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGgc                                                                         <  6:281677/147‑1 (MQ=255)
                                                                             gCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTg                                                                                    >  2:214876/1‑134 (MQ=255)
                                                                             gCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTg                                                                                    <  1:214876/134‑1 (MQ=255)
                                                                              cACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGgcgc                                                                       <  4:158643/146‑1 (MQ=255)
                                                                              cACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCAACGGCCCAGCTTGGCCGAATAGTAATAACGCAGCAATTCCGGCTGCAGCTGGTCCAGGTAGCTGCGGGCCTTGATGAAGGTGCAGCGCAACTTGGACATCTTGGCGCCGt                                                                    >  5:227034/1‑149 (MQ=255)
                                                                               aCGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCAGCGCGACTTGGACATCTTGGCGCCGtt                                                                   <  7:180491/149‑1 (MQ=255)
                                                                                cGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGgcg                                                                        >  8:50081/1‑143 (MQ=255)
                                                                                cGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGgcg                                                                        <  7:50081/143‑1 (MQ=255)
                                                                                cGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGtt                                                                   >  2:223149/1‑148 (MQ=255)
                                                                                 gAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGa                                                                 >  3:175836/1‑149 (MQ=255)
                                                                                 gAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGa                                                                 >  3:253544/1‑149 (MQ=255)
                                                                                 gAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGa                                                                 <  1:223149/149‑1 (MQ=255)
                                                                                          ccAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCa                                                           <  2:143627/147‑1 (MQ=255)
                                                                                          ccAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGAGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAg                                                          <  7:15017/148‑1 (MQ=255)
                                                                                           cAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAgg                                                         >  7:177210/1‑148 (MQ=255)
                                                                                              gTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCGAGGTAGGTGCGGGCCTGGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGc                                                     >  6:169676/1‑149 (MQ=255)
                                                                                                  aggtccagggcGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGt                                                  <  6:227034/148‑1 (MQ=255)
                                                                                                    gtccaggtcGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGc                                                <  8:12046/148‑1 (MQ=255)
                                                                                                     tccaggtcGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCcgcgcg                                                                                         >  4:209562/1‑106 (MQ=255)
                                                                                                     tccaggtcGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCcgcgcg                                                                                         <  3:209562/106‑1 (MQ=255)
                                                                                                          gtcgtcGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACGTGGCCATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGa                                         >  7:286930/1‑149 (MQ=255)
                                                                                                            cgtcgACGCCCCGGCCCAGCGTGGCCGCATACTAATACCCCCGGTATTCCGGCTGCAGGTGGTCCCCGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCCTCAGGTAGCCGTGCACGTTGAcc                                       <  4:281802/149‑1 (MQ=255)
                                                                                                            cgtcgACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGAcc                                       >  5:130928/1‑149 (MQ=255)
                                                                                                             gtcgACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGAcc                                       >  3:281802/1‑148 (MQ=255)
                                                                                                                gACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACcgcg                                    <  4:253544/148‑1 (MQ=255)
                                                                                                                 aCGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACCGCGGt                                  <  2:297559/149‑1 (MQ=255)
                                                                                                                   gCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACCGCGGTCg                                <  8:177210/149‑1 (MQ=255)
                                                                                                                       cGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTg                                                                  >  6:30787/1‑111 (MQ=255)
                                                                                                                                 tGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGAcc                                       <  6:245318/128‑1 (MQ=255)
                                                                                                                                 tGGCCGCATAGTAATAACGCAGGTATTCAGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGAcc                                       >  5:245318/1‑128 (MQ=255)
                                                                                                                                     cGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCAGgcgc              >  8:169020/1‑149 (MQ=255)
                                                                                                                                        aTAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCtt           >  1:282147/1‑149 (MQ=255)
                                                                                                                                             aataaCGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAg       >  3:13410/1‑148 (MQ=255)
                                                                                                                                                 aCGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATgg  <  2:256342/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTTGCCCTTGTGGATGAAACCGGCGCAGCGGCTGGCAATGTTGACGACCTTGCCGACCAGGTCGGAGTTGACCTTCTGCACGAAG‑TCTTCCAGGTTCAGGTCCAGGTCGTCGACGCCACGGCCCAGCTTGGCCGCATAGTAATAACGCAGGTATTCCGGCTGCAGGTGGTCCAGGTAGGTGCGGGCCTTGATGAAGGTGCCGCGCGACTTGGACATCTTGGCGCCGTTGACCGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGG  >  NC_002947/1254292‑1254584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑