Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,256,884	A→T	6.8%	noncoding (431/451 nt)	PP_mr14 ←	cspA regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,256,884	0	A	T	6.8%	250.4 / 4.6	83	noncoding (431/451 nt)	PP_mr14	cspA regulatory region
Reads supporting (aligned to +/- strand):  ref base A (39/38);  new base T (3/3);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCT  >  NC_002947/1256737‑1257029                                                                                                                                                     |                                                                                                                                                  ccATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAg                                                                                                                                                  >  3:247799/1‑149 (MQ=255)  cATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAgg                                                                                                                                                 <  7:211196/149‑1 (MQ=255)      aGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCgg                                                                                                                                              <  8:98694/148‑1 (MQ=255)         gAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGttt                                                                                                                                          <  7:200229/149‑1 (MQ=255)          aaGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGtttt                                                                                                                                         >  5:269504/1‑149 (MQ=255)              cTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                      >  1:297888/1‑148 (MQ=255)                 aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGttttt                                                                                                                                        <  4:63110/143‑1 (MQ=255)                 aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGttttt                                                                                                                                        >  3:63110/1‑143 (MQ=255)                 aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCACCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                        >  4:77937/1‑143 (MQ=255)                 aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCACCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                        >  4:73651/1‑143 (MQ=255)                 aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCACCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                        <  3:77937/143‑1 (MQ=255)                 aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCACCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                        <  3:73651/143‑1 (MQ=255)                  ccTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGc                                                                                                                                                >  1:124231/1‑134 (MQ=255)                     gCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                              >  8:195328/1‑149 (MQ=255)                      ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                      >  5:192214/1‑140 (MQ=255)                      ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                      <  6:192214/140‑1 (MQ=255)                      ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAg                                                                                                                             >  3:182074/1‑149 (MQ=255)                           ccGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGc                                                                                                                        <  7:213609/149‑1 (MQ=255)                            cGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGc                                                                                                                        >  7:194556/1‑148 (MQ=255)                                  tGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATc                                                                                                                  <  7:62998/148‑1 (MQ=255)                                     ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGCCt                                                                                                              <  6:306250/149‑1 (MQ=255)                                     ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACt                                                                                                              <  7:195328/149‑1 (MQ=255)                                      cGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                             <  4:182074/149‑1 (MQ=255)                                       gCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTa                                                                                                            <  6:269504/149‑1 (MQ=255)                                         acacaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAgg                                                                                                          <  4:167975/149‑1 (MQ=255)                                             aGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGAt                                                                                                                   <  3:166853/136‑1 (MQ=255)                                             aGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGAt                                                                                                                   >  4:166853/1‑136 (MQ=255)                                                      gcCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGaa                                                                                                 <  6:204715/145‑1 (MQ=255)                                                      gcCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGaa                                                                                                 >  5:204715/1‑145 (MQ=255)                                                         gTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACttcttc                                                                                          <  8:20212/149‑1 (MQ=255)                                                           aCTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAg                                                                                        >  6:116339/1‑149 (MQ=255)                                                               gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGTCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCt                                                                                     >  1:55830/1‑149 (MQ=255)                                                               gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGc                                                                                                         <  6:156257/128‑1 (MQ=255)                                                               gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGc                                                                                                         >  5:156257/1‑128 (MQ=255)                                                               gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTg                                                                                    >  5:291302/1‑149 (MQ=255)                                                                     tgtgCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGcc                                                                              >  6:29073/1‑149 (MQ=255)                                                                        gtaGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCttt                                                                           >  7:263631/3‑149 (MQ=255)                                                                         cAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTc                                                                          >  4:10677/1‑149 (MQ=255)                                                                              ccTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGcc                                                                     >  6:163643/1‑149 (MQ=255)                                                                              ccTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGcc                                                                     >  8:299225/1‑149 (MQ=255)                                                                              ccTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGAGTGTAGGCTAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGcc                                                                     >  1:102366/1‑149 (MQ=255)                                                                                  tgGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCa                                                                                         <  5:65417/125‑1 (MQ=255)                                                                                  tgGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTAGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCa                                                                                         >  6:65417/1‑125 (MQ=255)                                                                                   gggAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCggg                                                                <  6:291302/149‑1 (MQ=255)                                                                                    ggAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCggg                                                                <  2:55830/148‑1 (MQ=255)                                                                                     gAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCggg                                                                >  4:279153/1‑147 (MQ=255)                                                                                          ggCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGcgac                                                         >  6:206658/1‑149 (MQ=255)                                                                                             gAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaa                                                      <  2:297888/149‑1 (MQ=255)                                                                                              aGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCTCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaaa                                                     <  4:247799/149‑1 (MQ=255)                                                                                              aGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaaa                                                     <  5:116339/149‑1 (MQ=255)                                                                                                   g‑cgAAGTGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAACGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCAt                                                                                 <  6:92626/116‑1 (MQ=255)                                                                                                   g‑cgAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTaa                                                 >  6:220090/1‑148 (MQ=255)                                                                                                     cgAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACa                                               <  8:194556/149‑1 (MQ=255)                                                                                                       aaGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGc                                             <  7:299225/149‑1 (MQ=255)                                                                                                           ggCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTgg                                         <  5:29073/149‑1 (MQ=255)                                                                                                              cAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCct                                      >  3:243047/1‑149 (MQ=255)                                                                                                              cAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCCTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCct                                      >  5:127079/1‑149 (MQ=255)                                                                                                                    cTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCttt                                 >  3:251385/1‑148 (MQ=255)                                                                                                                    cTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCttt                                 >  1:200702/1‑148 (MQ=255)                                                                                                                       tttCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAgg                             <  2:37279/149‑1 (MQ=255)                                                                                                                       tttCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAgg                             >  1:37279/1‑149 (MQ=255)                                                                                                                       tttCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAg                              <  3:279153/148‑1 (MQ=255)                                                                                                                       tttCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCACGGGTAGCGACCAAAGTAACAGCCTGGCCTTCTTTCAgg                             <  6:88450/149‑1 (MQ=255)                                                                                                                         tCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAggg                            <  4:251385/148‑1 (MQ=255)                                                                                                                            aGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCCTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                        <  6:127079/149‑1 (MQ=255)                                                                                                                             gCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTg                       <  8:263631/149‑1 (MQ=255)                                                                                                                             gCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGACGCGGGTAGCGACGAAAGTAACAGACTGGCCATCTTTCATGGattt                        >  4:83827/1‑148 (MQ=255)                                                                                                                                ggCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGTGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAg                    <  1:278934/149‑1 (MQ=255)                                                                                                                                   aTAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTa                                                              >  7:33563/1‑104 (MQ=255)                                                                                                                                   aTAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTa                                                              <  8:33563/104‑1 (MQ=255)                                                                                                                                   aTAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCg                 >  2:61433/1‑149 (MQ=255)                                                                                                                                     aaaaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                >  6:284937/1‑148 (MQ=255)                                                                                                                                      aaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                        <  1:143578/139‑1 (MQ=255)                                                                                                                                      aaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                        >  2:143578/1‑139 (MQ=255)                                                                                                                                       aaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAg             >  7:233317/1‑149 (MQ=255)                                                                                                                                       aaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAg             >  3:116474/1‑149 (MQ=255)                                                                                                                                            cccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTgg        <  3:10677/149‑1 (MQ=255)                                                                                                                                             ccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGa       <  2:102366/149‑1 (MQ=255)                                                                                                                                             ccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGa       >  6:268147/1‑149 (MQ=255)                                                                                                                                              cGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCa              <  7:128950/141‑1 (MQ=255)                                                                                                                                              cGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCa              >  8:128950/1‑141 (MQ=255)                                                                                                                                              cGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAt      >  2:35635/1‑149 (MQ=255)                                                                                                                                                  aGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCt  <  1:61433/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCG‑CGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCT  >  NC_002947/1256737‑1257029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑