| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,281,194 | A→T | 13.5% | intergenic (+20/+23) | PP_5742 → / ← PP_1118 | membrane protein/recombinase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,281,194 | 0 | A | T | 13.5% | 123.4 / 11.6 | 52 | intergenic (+20/+23) | PP_5742/PP_1118 | membrane protein/recombinase‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (22/23); new base T (3/4); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01 | |||||||||||
TGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCAACTGAA > NC_002947/1281050‑1281335 | tGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCg > 3:195695/1‑149 (MQ=255)tGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCGATGGGACGCTAGACCAAACCGAATTTAGAGAGCCCGCCATAAGGCg > 6:250371/1‑149 (MQ=255) ggggTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCgg > 7:91349/1‑149 (MQ=255) gggTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCgg < 6:280040/148‑1 (MQ=255) gggTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGCGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCTTATGGCggg < 7:71968/149‑1 (MQ=255) gTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCgg < 7:138911/146‑1 (MQ=255) gTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCgg > 8:138911/1‑146 (MQ=255) tATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAATCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTc > 7:69990/1‑148 (MQ=255) aCTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCTTATGGCgggctctct < 2:304586/148‑7 (MQ=255) cTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCtattat < 4:195695/149‑1 (MQ=255) tgATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCa < 1:44765/146‑1 (MQ=255) aTTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTAttt < 8:69990/149‑1 (MQ=255) gCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTc > 1:47835/1‑149 (MQ=255) cAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTc < 1:306409/148‑1 (MQ=255) cAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTAATAATATGTCt > 8:13117/1‑149 (MQ=255) tATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGataa > 5:104017/1‑134 (MQ=255) tgtgTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTcc < 8:91349/147‑1 (MQ=255) tttttGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCAGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATgg > 5:216710/1‑149 (MQ=255) gaTGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCca > 8:163906/1‑133 (MQ=255) gaTGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCca < 7:163906/133‑1 (MQ=255) gTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACAAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTg > 8:85424/1‑149 (MQ=255) ttGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCtattat < 8:209015/79‑1 (MQ=255) ttGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCtattat > 7:209015/1‑79 (MQ=255) gCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGa < 2:47835/148‑1 (MQ=255) cATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGAtt < 5:226491/149‑1 (MQ=255) ccACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGc < 4:113662/127‑1 (MQ=255) ccACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGc > 3:113662/1‑127 (MQ=255) acaGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCca > 7:297171/1‑106 (MQ=255) acaGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCca < 8:297171/106‑1 (MQ=255) acaGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGtt > 2:316716/1‑149 (MQ=255) aGAAGCCTCTTCTATGGGAAGCTAGACCAAACCTAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGtttt < 5:250371/149‑1 (MQ=255) aGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTgg > 5:6572/1‑148 (MQ=255) cTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAggcg < 6:6572/148‑1 (MQ=255) gCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTcc < 8:59345/145‑1 (MQ=255) gCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTcc > 7:59345/1‑145 (MQ=255) gCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGAGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCt > 2:155800/1‑149 (MQ=255) gACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCg < 4:149142/147‑1 (MQ=255) gACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGtt < 2:297055/149‑1 (MQ=255) ccAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGtt > 4:105133/1‑121 (MQ=255) ccAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGtt < 3:105133/121‑1 (MQ=255) ccAAACTGAATTAAGAGAGCCCGCCATAAGGAGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGCTTCAGTTTTGGCAAAGGCGGCTTCCACCTCGtt > 4:298246/1‑147 (MQ=255) aaaCTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAgg < 7:85424/149‑1 (MQ=255) aTTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTAATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCtt < 7:13117/149‑1 (MQ=255) agagagCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCATGATTCAGTTTTGGCAAAGGCGGCTTCAACCTCGTTAAGGTCGCCTTggg > 3:39222/1‑148 (MQ=255) gacccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAg < 4:260254/131‑1 (MQ=255) gacccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAg > 3:260254/3‑133 (MQ=255) acccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCaa < 2:4945/146‑1 (MQ=255) acccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCaa > 1:4945/2‑147 (MQ=255) cccGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGt > 2:132582/1‑88 (MQ=255) cccGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGt < 1:132582/88‑1 (MQ=255) ccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCAACTg > 7:227717/1‑148 (MQ=255) cGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCAACTGaa < 1:316716/149‑1 (MQ=255) | TGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCAACTGAA > NC_002947/1281050‑1281335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |