Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,294,116 A→G 9.8% intergenic (+17/+23) pdxH → / ← PP_1130 pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,294,1160AG 9.8% 134.3 / 5.6 51intergenic (+17/+23)pdxH/PP_1130pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base G (2/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

AGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGA  >  NC_002947/1293969‑1294253
                                                                                                                                                   |                                                                                                                                         
aGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAg                                                                                                                                          <  3:27166/149‑1 (MQ=255)
   aTTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCgg                                                                                                                                       <  4:100212/149‑1 (MQ=255)
        gggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCa                                                                                                                                  <  4:138911/149‑1 (MQ=255)
          gggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAAATATCGGGTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGc                                                                                                                                >  1:11373/1‑149 (MQ=255)
           ggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGGCTGCGTGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCAgtt                                                                                                                               <  8:133478/149‑3 (MQ=255)
                           ccGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTAccc                                                                                                               >  3:13824/1‑149 (MQ=255)
                             gAGCGCATCGAGTTCTGGCAGTGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTAcccc                                                                                                              <  7:148991/148‑1 (MQ=255)
                             gAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTAcccc                                                                                                              >  8:148991/1‑148 (MQ=255)
                                   aTCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGGTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAAc                                                                                                       <  4:166336/149‑1 (MQ=255)
                                      gAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCa                                                                                                                                  >  3:161747/1‑119 (MQ=255)
                                      gAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCa                                                                                                                                  <  4:161747/119‑1 (MQ=255)
                                              gCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTcg                                                                                            >  1:303361/1‑149 (MQ=255)
                                              gCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTcg                                                                                            >  5:19578/1‑149 (MQ=255)
                                              gCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTcg                                                                                            >  1:117393/1‑149 (MQ=255)
                                              gCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTc                                                                                             <  6:186262/148‑1 (MQ=255)
                                               cAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGCACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTcgc                                                                                           <  1:244674/149‑1 (MQ=255)
                                                    gcgggcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGCTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTcc                                                                                      <  6:97384/149‑1 (MQ=255)
                                                         cAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAacca                                                                                 >  5:277638/1‑149 (MQ=255)
                                                                 cTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGc                                                                           >  3:72868/1‑147 (MQ=255)
                                                                    cATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                            >  4:77041/1‑95 (MQ=255)
                                                                    cATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                            <  3:77041/95‑1 (MQ=255)
                                                                       gATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTcc                                                                                      <  8:241952/130‑1 (MQ=255)
                                                                       gATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTcc                                                                                      >  7:241952/1‑130 (MQ=255)
                                                                       gATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCAccc                                                                              <  6:220162/138‑1 (MQ=255)
                                                                       gATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCAccc                                                                              >  5:220162/1‑138 (MQ=255)
                                                                        aTCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCt                                                                   <  4:13824/148‑1 (MQ=255)
                                                                           gTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTAc                                                                <  4:72868/148‑1 (MQ=255)
                                                                            tttGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGc                                                                    <  8:226330/143‑1 (MQ=255)
                                                                            tttGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGc                                                                    >  8:159445/1‑143 (MQ=255)
                                                                            tttGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGc                                                                    >  7:226330/1‑143 (MQ=255)
                                                                            tttGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGc                                                                    <  7:159445/143‑1 (MQ=255)
                                                                               gAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGtt                                                                      <  4:13571/138‑1 (MQ=255)
                                                                               gAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGtt                                                                      >  3:13571/1‑138 (MQ=255)
                                                                                     tCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTtc                                                     >  4:80563/1‑149 (MQ=255)
                                                                                              ggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCcgc                                            >  6:14717/1‑149 (MQ=255)
                                                                                                    gCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTgc                                      >  6:84205/1‑149 (MQ=255)
                                                                                                      aGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTgcgc                                    <  3:230753/149‑1 (MQ=255)
                                                                                                      aGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTgcgc                                    <  4:102014/149‑1 (MQ=255)
                                                                                                          gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAAc                                <  6:277638/149‑1 (MQ=255)
                                                                                                          gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAAc                                >  2:156531/1‑149 (MQ=255)
                                                                                                                     ggCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCAt                     >  1:75022/1‑149 (MQ=255)
                                                                                                                      gCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATg                    <  5:84205/149‑1 (MQ=255)
                                                                                                                        tGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAATCATCAAGCTACCCCGGATAACAAGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTAAGCCCCAACTTCTCCTTCCGCGCCTCCGCCAACCTATCCAGCAAGaa                  >  1:294612/1‑149 (MQ=255)
                                                                                                                                tGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGtt          >  1:146013/1‑149 (MQ=255)
                                                                                                                                   gCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGttttt       <  6:123113/149‑1 (MQ=255)
                                                                                                                                      gcTGGGGCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAg    >  6:245894/2‑149 (MQ=255)
                                                                                                                                      aCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAg    >  6:95096/1‑149 (MQ=255)
                                                                                                                                      aCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAg    >  4:6799/1‑149 (MQ=255)
                                                                                                                                        tGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGa  >  1:17373/1‑149 (MQ=255)
                                                                                                                                               gccAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAAc                                >  6:270352/1‑112 (MQ=255)
                                                                                                                                               gccAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAAc                                <  5:270352/112‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
AGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGA  >  NC_002947/1293969‑1294253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑