Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,299,470	C→A	5.8%	L236L (CTG→CTT)	PP_1134 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,299,470	0	C	A	5.8%	221.6 / 2.3	68	L236L (CTG→CTT)	PP_1134	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/29);  new base A (2/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCACCGGCCTTGAC  >  NC_002947/1299332‑1299616                                                                                                                                           |                                                                                                                                                   ttCCATGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGa                                                                                                                                          >  5:283620/1‑149 (MQ=255) ttCCATGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGa                                                                                                                                          >  4:150228/1‑149 (MQ=255)    cATGAAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGAcc                                                                                                                                        <  5:80523/148‑1 (MQ=255)     aTGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCtt                                                                                                                                      >  2:189665/1‑149 (MQ=255)      tGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTtc                                                                                                                                     <  6:260870/149‑1 (MQ=255)      tGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAAAAGGTCGGTGAcc                                                                                                                                        >  4:80617/1‑146 (MQ=255)      tGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAAAAGGTCGGTGAcc                                                                                                                                        >  4:76383/1‑146 (MQ=255)      tGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAAAAGGTCGGTGAcc                                                                                                                                        <  3:80617/146‑1 (MQ=255)      tGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAAAAGGTCGGTGAcc                                                                                                                                        <  3:76383/146‑1 (MQ=255)        tAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTtctc                                                                                                                                   >  6:287762/1‑149 (MQ=255)             acCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTgg                                                                                                                               >  2:74700/1‑148 (MQ=255)             acCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTgg                                                                                                                               >  2:78824/1‑148 (MQ=255)              ccTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGaa                                                                                                                             <  1:214122/149‑1 (MQ=255)                      gTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACtt                                                                                                                     >  4:260473/1‑149 (MQ=255)                         cagcaTGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCg                                                                                                                   <  2:84264/148‑1 (MQ=255)                              tGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGccccc                                                                                                             <  1:189665/149‑1 (MQ=255)                                         tCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCTCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGGGCCCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCag                                                                                                  >  5:78142/1‑148 (MQ=255)                                         gcgcgGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCAt                                                                                                  <  3:260473/148‑1 (MQ=255)                                                       gCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGgcgc                                                                                        <  5:196641/145‑1 (MQ=255)                                                       gCCCAGGGCCAATACCACATGGCGAGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGgcgc                                                                                        >  6:196641/1‑145 (MQ=255)                                                         ccAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGt                                                                                  >  3:132214/1‑149 (MQ=255)                                                          cAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGtt                                                                                 <  5:287762/149‑1 (MQ=255)                                                               ccAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGATGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGAcc                                                                            <  1:74700/149‑1 (MQ=255)                                                               ccAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGATGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGAcc                                                                            <  1:78824/149‑1 (MQ=255)                                                                  aTACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATg                                                                         >  1:64206/1‑149 (MQ=255)                                                                    aCCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGcc                                                                       >  5:167868/1‑149 (MQ=255)                                                                      cacaTGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCgg                                                                     >  4:29333/1‑149 (MQ=255)                                                                        caTGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTg                                                                                                                                >  5:130077/1‑88 (MQ=255)                                                                        caTGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTg                                                                                                                                <  6:130077/88‑1 (MQ=255)                                                                         aTGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGa                                                                  <  5:5466/149‑1 (MQ=255)                                                                         aTGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGa                                                                  <  5:147678/149‑1 (MQ=255)                                                                            gcgcGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAgg                                                                <  3:289862/148‑1 (MQ=255)                                                                              gcgAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCg                                                              >  2:136071/1‑148 (MQ=255)                                                                                gAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGa                                                                                                      <  6:171194/106‑1 (MQ=255)                                                                                gAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGa                                                                                                      >  5:171194/1‑106 (MQ=255)                                                                                gAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGaa                                                           <  2:58002/149‑1 (MQ=255)                                                                                gAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGaa                                                           <  3:29333/149‑1 (MQ=255)                                                                                 aGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAg                                                          <  2:64206/149‑1 (MQ=255)                                                                                    gCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTcc                                                                                                                                  >  4:80436/1‑74 (MQ=255)                                                                                    gCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTcc                                                                                                                                  <  3:80436/74‑1 (MQ=255)                                                                                           cTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGAt                                                  <  6:167868/147‑1 (MQ=255)                                                                                              gccgcTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGc                                              <  6:283620/148‑1 (MQ=255)                                                                                              gccgcTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCg                                             >  4:210608/1‑149 (MQ=255)                                                                                                cgcTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGc                                           >  4:246365/1‑149 (MQ=255)                                                                                                 gcTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGCTCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCt                                          >  4:189506/1‑149 (MQ=255)                                                                                                 gcTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGAcc                                                                            <  7:29163/115‑1 (MQ=255)                                                                                                 gcTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGAcc                                                                            >  8:29163/1‑115 (MQ=255)                                                                                                           aCTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTa                                >  4:33544/1‑149 (MQ=255)                                                                                                              acacCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAg                             >  5:139075/1‑149 (MQ=255)                                                                                                               cacCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACa                              <  4:132214/147‑1 (MQ=255)                                                                                                                acCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATGATGTCCTGGCGCATCTGTTCGACCATGc                                                                        <  1:180639/104‑1 (MQ=255)                                                                                                                acCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGc                                                                        >  2:180639/1‑104 (MQ=255)                                                                                                                         ttGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGAtt                         <  7:293947/142‑1 (MQ=255)                                                                                                                         ttGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGAtt                         >  8:293947/1‑142 (MQ=255)                                                                                                                           ggCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTgg                 <  3:246365/148‑1 (MQ=255)                                                                                                                              cATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCa             >  8:1072/1‑149 (MQ=255)                                                                                                                               aTCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCAc            >  6:288650/1‑149 (MQ=255)                                                                                                                               aTCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCAc            >  2:32200/1‑149 (MQ=255)                                                                                                                                tCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCAcc           >  2:282198/1‑149 (MQ=255)                                                                                                                                 cTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGGCTGGCGCACCg          >  6:148142/1‑149 (MQ=255)                                                                                                                                   tCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCttgttg                                     <  7:177696/120‑1 (MQ=255)                                                                                                                                   tCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCttgttg                                     >  8:177696/1‑120 (MQ=255)                                                                                                                                        cAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCACCGGCCt      <  5:305573/146‑1 (MQ=255)                                                                                                                                        cAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCACCGGCCt      >  6:305573/1‑146 (MQ=255)                                                                                                                                          aCAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCACCGGCCtt     >  5:67740/1‑145 (MQ=255)                                                                                                                                          aCAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCACCGGCCtt     <  6:67740/145‑1 (MQ=255)                                                                                                                                          aCAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCACCGGCCTTGAc  >  5:210182/1‑148 (MQ=255)                                                                                                                                          aCAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAACGTGCCGATGTGCGGCTTGATGATGTACAGGATTTCGTCTGGCGCACCGGCCTTGAc  >  2:110006/1‑148 (MQ=255)                                                                                                                                           cAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAg                             >  4:115577/1‑120 (MQ=255)                                                                                                                                           cAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAg                             <  3:115577/120‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                   TTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAAGGTGTCGCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCCCAGGGCGATCATGTCCTGGCGCATCTGTTCGACCATGCCGGTGAGGCGGAAGGTGCCGATGTGCGGCTTGTTGATGTACAGGATTTCGTCTGGCGCACCGGCCTTGAC  >  NC_002947/1299332‑1299616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑