Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,317,134 G→A 7.2% L184L (CTG→CTA)  PP_1150 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,317,1340GA 7.2% 161.9 / 2.3 55L184L (CTG→CTA) PP_1150membrane protein
Reads supporting (aligned to +/- strand):  ref base G (21/30);  new base A (2/2);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

CAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGT  >  NC_002947/1316991‑1317272
                                                                                                                                               |                                                                                                                                          
gaaGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAg                                                                                                                                         <  2:28874/146‑1 (MQ=255)
cAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCTGTGCCTTGCGCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAgg                                                                                                                                        <  6:13879/148‑1 (MQ=255)
cAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGTCCTGGCAGAACGGCCTGTAg                                                                                                                                         <  7:136325/147‑1 (MQ=255)
cAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAg                                                                                                                                         <  2:175258/147‑1 (MQ=255)
         gACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAgcgc                                                                                                                              >  6:219966/1‑149 (MQ=255)
          tccAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGcc                                                                                                                             <  3:30069/148‑1 (MQ=255)
          aCCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGCGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAgcgc                                                                                                                              >  8:288651/1‑148 (MQ=255)
                ccGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTg                                                                                                                       <  7:160602/149‑1 (MQ=255)
                       tCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCg                                                                                                                   >  7:164994/1‑146 (MQ=255)
                                cGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAgg                                                                                                       >  2:207793/1‑149 (MQ=255)
                                 gagaTCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGgc                                                                                                      <  3:69549/149‑1 (MQ=255)
                                       tGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAg                                                                                                        >  3:251083/1‑141 (MQ=255)
                                        gCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCa                                                                                                 >  5:193182/1‑147 (MQ=255)
                                        gCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGCGTCGAGCAGCAACTCGGCGCGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCCGCGCCATCCAGCGCGGAGAGGACGAGGCGCGCa                                                                                                 >  8:134811/1‑147 (MQ=255)
                                           tGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGc                                                                                            >  4:273213/1‑149 (MQ=255)
                                                 ttttCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCctgctg                                                                                      <  5:294194/149‑1 (MQ=255)
                                                     cTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGa                                                                                    >  7:256593/1‑147 (MQ=255)
                                                      tgtgCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAg                                                                                 >  4:59673/1‑149 (MQ=255)
                                                      tgtgCAACAGCCAGCAGTGCCTTGCCCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAg                                                                                 >  3:308401/1‑149 (MQ=255)
                                                       gtgCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAg                                                                                 <  6:27412/148‑1 (MQ=255)
                                                       gtgCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGAGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGc                                                                                >  8:228377/1‑149 (MQ=255)
                                                        tgCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGaaa                                                                                  <  5:137283/146‑1 (MQ=255)
                                                        tgCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGaaa                                                                                  <  6:28521/146‑1 (MQ=255)
                                                          cAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTg                                                                             <  6:193182/149‑1 (MQ=255)
                                                          cAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTACAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTg                                                                             >  5:177056/1‑149 (MQ=255)
                                                          cAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTACAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTg                                                                             >  5:181560/1‑149 (MQ=255)
                                                                  agcagTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCAt                                                                     >  2:80807/1‑149 (MQ=255)
                                                                  agaagTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGCCAGAACGGCCTGCAGGCCGCCAGAGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCAGGACAGCCTGGGGCGCAt                                                                     >  7:234030/4‑149 (MQ=255)
                                                                   gtagTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATc                                                                    >  8:268488/3‑149 (MQ=255)
                                                                      gTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGtt                                                                 <  5:219966/149‑1 (MQ=255)
                                                                      gTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGtt                                                                 <  8:79097/149‑1 (MQ=255)
                                                                       tGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTg                                                                <  1:138494/149‑1 (MQ=255)
                                                                         ccTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTACAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGc                                                               <  6:177056/148‑1 (MQ=255)
                                                                         ccTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTACAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGc                                                               <  6:181560/148‑1 (MQ=255)
                                                                              ctctGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGa                                                         <  3:273213/149‑1 (MQ=255)
                                                                                   gCCCTTGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACa                                                       <  7:134811/146‑1 (MQ=255)
                                                                                     ccATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCaa                                                  <  8:164994/149‑1 (MQ=255)
                                                                                     ccATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTCCCATCGACAGCCaa                                                  <  7:268488/149‑1 (MQ=255)
                                                                                      cATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAAc                                                 <  4:60788/149‑1 (MQ=255)
                                                                                            ggTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAAc                                           <  3:59673/149‑1 (MQ=255)
                                                                                              tCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACAt                                         <  7:228377/149‑1 (MQ=255)
                                                                                              tCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGCCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACAt                                         <  1:257575/149‑1 (MQ=255)
                                                                                                gAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTggg                                                                           <  4:304116/113‑1 (MQ=255)
                                                                                                gAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTggg                                                                           >  3:304116/1‑113 (MQ=255)
                                                                                                   cagcaACTGTGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTg                                    <  1:207793/149‑1 (MQ=255)
                                                                                                      caACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCt                                     <  6:136843/145‑1 (MQ=255)
                                                                                                      caACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCt                                     >  5:136843/1‑145 (MQ=255)
                                                                                                            ggcgggcAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAgg                            <  7:288651/148‑1 (MQ=255)
                                                                                                                 gcAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACAt                                         <  6:184869/130‑1 (MQ=255)
                                                                                                                 gcAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACAt                                         >  5:184869/1‑130 (MQ=255)
                                                                                                                  cAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATcg                     >  4:65233/1‑149 (MQ=255)
                                                                                                                     gcgcGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCgg                  <  2:80425/149‑1 (MQ=255)
                                                                                                                                cTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAAcgtg       >  4:245270/1‑149 (MQ=255)
                                                                                                                                   gCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAAcgtgccc    >  3:307201/1‑149 (MQ=255)
                                                                                                                                     aGAACGGCCTGCAGGCCGGCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAAcgtgcccgt  <  1:211602/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                          
CAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAGGCCTGGCAGAACGGCCTGCAGGCCGCCAGCGCCATGCTGGGCGGAGAGGACGAGGCGCGCAAAGGCCTGCTGGAAAGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGT  >  NC_002947/1316991‑1317272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑