| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,319,015 | A→T | 8.3% | Q80L (CAG→CTG) | PP_1152 → | membrane fusion efflux protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,319,015 | 0 | A | T | 8.3% | 177.3 / 5.6 | 60 | Q80L (CAG→CTG) | PP_1152 | membrane fusion efflux protein |
| Reads supporting (aligned to +/- strand): ref base A (28/27); new base T (3/2); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01 | |||||||||||
GTGATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATGCC > NC_002947/1318880‑1319157 | gTGATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAgg > 6:148330/1‑138 (MQ=255)gTGATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAgg < 5:148330/138‑1 (MQ=255) ccGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCa < 2:94165/149‑1 (MQ=255) gcCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGAc > 7:44496/1‑149 (MQ=255) tggtgATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACAcgc > 8:50647/1‑149 (MQ=255) tGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTgc > 7:161976/1‑149 (MQ=255) gtgtCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCga < 1:138383/125‑1 (MQ=255) gtgtCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCga > 2:138383/1‑125 (MQ=255) gtgtCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACATGCGCCCAGCAACTgc < 7:33643/145‑1 (MQ=255) gtgtCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACATGCGCCCAGCAACTgc > 8:33643/1‑145 (MQ=255) tgtCCGGCTGGGTGCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCtt < 2:94135/148‑1 (MQ=255) tCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCa < 8:83328/104‑1 (MQ=255) tCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCa > 7:83328/1‑104 (MQ=255) gcgAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAag > 5:49410/1‑147 (MQ=255) tCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGcc < 2:294025/146‑1 (MQ=255) tCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGcc > 1:294025/1‑146 (MQ=255) aaGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCg > 8:142166/1‑149 (MQ=255) aaGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCg > 5:265686/1‑149 (MQ=255) ccATGACAAGCAGGAGTTCAAGGCTGGCGTCTTGTTTATGAGTATTGACCGCGAGCGCTTACAGGCAGCGCTCGATCAGGCCAGCGCGTTGACCGATACAGGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACt < 3:37164/148‑1 (MQ=255) ccATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTg > 8:108548/1‑149 (MQ=255) aCAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGcttc < 6:265686/148‑4 (MQ=255) cAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTgg < 8:161976/149‑1 (MQ=255) cagcagGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTg < 2:12415/139‑1 (MQ=255) cagcagGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTg > 1:12415/1‑139 (MQ=255) cagGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCga > 8:105242/1‑83 (MQ=255) gTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGc > 4:128391/1‑149 (MQ=255) gTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGc > 8:54833/1‑149 (MQ=255) tCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCACGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGc > 5:223305/1‑148 (MQ=255) aaGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGa < 6:49410/148‑1 (MQ=255) cTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGAt > 8:167730/1‑145 (MQ=255) cTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGAt < 7:167730/145‑1 (MQ=255) cGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGcc > 1:126551/1‑148 (MQ=255) ttgttgATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGtt > 5:304369/1‑149 (MQ=255) ttgATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGc < 7:237629/148‑1 (MQ=255) ttgATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCg < 7:142166/149‑1 (MQ=255) gatgaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGt < 3:153333/148‑1 (MQ=255) tgaGTATTGACCGCGAGCGGTTCCAGGGCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGaa < 7:102227/149‑1 (MQ=255) tgaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGaa < 3:41886/149‑1 (MQ=255) tgaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGa > 6:12284/1‑148 (MQ=255) gaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTgga > 5:303610/1‑146 (MQ=255) gaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGaa > 8:101269/1‑148 (MQ=255) aGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGaaaa > 2:192586/1‑149 (MQ=255) gACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGcc > 8:167714/1‑147 (MQ=255) aCCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCa < 6:304369/148‑1 (MQ=255) gatcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTggg < 5:134098/105‑1 (MQ=255) gatcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTggg > 6:134098/4‑108 (MQ=255) gatcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGAt > 3:100032/4‑120 (MQ=255) gatcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGAt < 4:100032/117‑1 (MQ=255) ccGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCagag < 3:112007/115‑1 (MQ=255) ccGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCagag < 3:116645/115‑1 (MQ=255) ccGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCagag > 4:116645/1‑115 (MQ=255) ccGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCagag > 4:112007/1‑115 (MQ=255) cgcgAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGAt < 7:54833/149‑1 (MQ=255) tcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGAt > 1:275379/2‑148 (MQ=255) gcgcTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATg < 7:50647/149‑1 (MQ=255) cgcTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATgc < 7:101269/149‑1 (MQ=255) gcTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATgcc < 5:12284/149‑1 (MQ=255) gcTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATgcc < 7:108548/149‑1 (MQ=255) ttCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAg > 8:124557/1‑120 (MQ=255) ttCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAg < 7:124557/120‑1 (MQ=255) | GTGATGCCCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATGCC > NC_002947/1318880‑1319157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |