Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,319,018 C→A 8.5% A81D (GCC→GAC) ‡ PP_1152 → membrane fusion efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,319,0180CA 8.5% 181.2 / 6.5 59A81D (GCC→GAC) ‡PP_1152membrane fusion efflux protein
Reads supporting (aligned to +/- strand):  ref base C (28/25);  new base A (3/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01

CCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATGCCGCCATCGC  >  NC_002947/1318887‑1319165
                                                                                                                                   |                                                                                                                                                   
ccGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCa                                                                                                                                    <  2:94165/149‑1 (MQ=255)
          gcCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGAc                                                                                                                          >  7:44496/1‑149 (MQ=255)
                    tggtgATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACAcgc                                                                                                                >  8:50647/1‑149 (MQ=255)
                                 tGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTgc                                                                                                   >  7:161976/1‑149 (MQ=255)
                                     gtgtCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCga                                                                                                                       <  1:138383/125‑1 (MQ=255)
                                     gtgtCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCga                                                                                                                       >  2:138383/1‑125 (MQ=255)
                                     gtgtCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACATGCGCCCAGCAACTgc                                                                                                   <  7:33643/145‑1 (MQ=255)
                                     gtgtCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACATGCGCCCAGCAACTgc                                                                                                   >  8:33643/1‑145 (MQ=255)
                                      tgtCCGGCTGGGTGCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCtt                                                                                               <  2:94135/148‑1 (MQ=255)
                                        tCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCa                                                                                                                                         >  7:83328/1‑104 (MQ=255)
                                        tCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCa                                                                                                                                         <  8:83328/104‑1 (MQ=255)
                                                     gcgAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAag                                                                                 >  5:49410/1‑147 (MQ=255)
                                                           tCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGcc                                                                            <  2:294025/146‑1 (MQ=255)
                                                           tCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGcc                                                                            >  1:294025/1‑146 (MQ=255)
                                                             aaGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCg                                                                       >  5:265686/1‑149 (MQ=255)
                                                             aaGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCg                                                                       >  8:142166/1‑149 (MQ=255)
                                                                  ccATGACAAGCAGGAGTTCAAGGCTGGCGTCTTGTTTATGAGTATTGACCGCGAGCGCTTACAGGCAGCGCTCGATCAGGCCAGCGCGTTGACCGATACAGGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACt                                                                   <  3:37164/148‑1 (MQ=255)
                                                                  ccATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTg                                                                  >  8:108548/1‑149 (MQ=255)
                                                                       aCAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGcttc                                                              <  6:265686/148‑4 (MQ=255)
                                                                        cAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTgg                                                            <  8:161976/149‑1 (MQ=255)
                                                                            cagcagGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTg                                                                  <  2:12415/139‑1 (MQ=255)
                                                                            cagcagGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTg                                                                  >  1:12415/1‑139 (MQ=255)
                                                                               cagGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCga                                                                                                                       >  8:105242/1‑83 (MQ=255)
                                                                                  gTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGc                                                  >  4:128391/1‑149 (MQ=255)
                                                                                  gTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGc                                                  >  8:54833/1‑149 (MQ=255)
                                                                                   tCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCACGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGc                                                  >  5:223305/1‑148 (MQ=255)
                                                                                     aaGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGa                                                <  6:49410/148‑1 (MQ=255)
                                                                                         cTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGAt                                               >  8:167730/1‑145 (MQ=255)
                                                                                         cTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGAt                                               <  7:167730/145‑1 (MQ=255)
                                                                                             cGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGcc                                        >  1:126551/1‑148 (MQ=255)
                                                                                                 ttgttgATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGtt                                   >  5:304369/1‑149 (MQ=255)
                                                                                                    ttgATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGc                                 <  7:237629/148‑1 (MQ=255)
                                                                                                    ttgATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCg                                <  7:142166/149‑1 (MQ=255)
                                                                                                      gatgaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGt                               <  3:153333/148‑1 (MQ=255)
                                                                                                        tgaGTATTGACCGCGAGCGGTTCCAGGGCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGaa                            <  7:102227/149‑1 (MQ=255)
                                                                                                        tgaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGaa                            <  3:41886/149‑1 (MQ=255)
                                                                                                        tgaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGa                             >  6:12284/1‑148 (MQ=255)
                                                                                                         gaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTgga                            >  5:303610/1‑146 (MQ=255)
                                                                                                         gaGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGaa                            >  8:101269/1‑148 (MQ=255)
                                                                                                          aGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGaaaa                          >  2:192586/1‑149 (MQ=255)
                                                                                                                gACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGcc                      >  8:167714/1‑147 (MQ=255)
                                                                                                                 aCCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCa                    <  6:304369/148‑1 (MQ=255)
                                                                                                                  gatcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTggg                                                           <  5:134098/105‑1 (MQ=255)
                                                                                                                  gatcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTggg                                                           >  6:134098/4‑108 (MQ=255)
                                                                                                                  gatcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGAt                                               >  3:100032/4‑120 (MQ=255)
                                                                                                                  gatcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGAt                                               <  4:100032/117‑1 (MQ=255)
                                                                                                                  ccGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCagag                                                    <  3:112007/115‑1 (MQ=255)
                                                                                                                  ccGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCagag                                                    >  4:116645/1‑115 (MQ=255)
                                                                                                                  ccGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCagag                                                    >  4:112007/1‑115 (MQ=255)
                                                                                                                  ccGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCagag                                                    <  3:116645/115‑1 (MQ=255)
                                                                                                                   cgcgAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGAt                 <  7:54833/149‑1 (MQ=255)
                                                                                                                    tcgAGCGCGGCCTGGAAGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGAt                 >  1:275379/2‑148 (MQ=255)
                                                                                                                        gcgcTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATg            <  7:50647/149‑1 (MQ=255)
                                                                                                                         cgcTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATgc           <  7:101269/149‑1 (MQ=255)
                                                                                                                          gcTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATgcc          <  5:12284/149‑1 (MQ=255)
                                                                                                                          gcTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATgcc          <  7:108548/149‑1 (MQ=255)
                                                                                                                            ttCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAg                                     >  8:124557/1‑120 (MQ=255)
                                                                                                                            ttCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAg                                     <  7:124557/120‑1 (MQ=255)
                                                                                                                                  gCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATGCCGCCATcgc  >  1:161666/1‑149 (MQ=255)
                                                                                                                                   |                                                                                                                                                   
CCGTGTGCGCGCCGATGTGGTGGTGATTGCGCCTGATGTGTCCGGCTGGGTTCGCGAACTCAAGGTCCATGACAACCAGCAGGTCAAGGCTGGCGACTTGTTGATGAGTATTGACCGCGAGCGCTTCCAGGCCGCGCTCGATCAGGCCAGCGCGGTGACCGAGACACGCGCCCAGCAACTGCGCTTGCGCGAGCGTGAAGCAGCCCGGCGTACTGCCCTGGGGCCAGAGGCGATCAGTGCCGAGTTGCGTGAAAACGCCCAGATCAATGCCGCCATCGC  >  NC_002947/1318887‑1319165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑