Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,352,304 G→A 6.4% G18G (GGC→GGT)  PP_1178 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,352,3040GA 6.4% 187.3 / 2.3 62G18G (GGC→GGT) PP_1178hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base A (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATA  >  NC_002947/1352157‑1352446
                                                                                                                                                   |                                                                                                                                              
tgtTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGc                                                                                                                                               <  4:177960/149‑1 (MQ=255)
tgtTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCCACAATGTGTGTCTTTGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGc                                                                                                                                               <  7:99693/149‑1 (MQ=255)
 gtTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGcc                                                                                                                                              >  5:4021/1‑149 (MQ=255)
  tttCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCg                                                                                                                                             >  7:29621/1‑149 (MQ=255)
      cTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGTGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGttt                                                                                                                                         <  6:4021/149‑1 (MQ=255)
       ttaGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGAGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGACAGGTTTc                                                                                                                                        >  3:267565/3‑149 (MQ=255)
       tGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGACCGGTTTc                                                                                                                                        >  3:267567/1‑149 (MQ=255)
             ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGtt                                                                                                                                  >  1:124811/1‑149 (MQ=255)
               gtgtTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGtt                                                                                                                                  <  7:131048/147‑1 (MQ=255)
                     gCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCaa                                                                                                                          >  4:239545/1‑149 (MQ=255)
                           tCGGCCTCACCGTGGGCTTGCGTGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTAGAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGACCGGTTTCTGCGTTGCGACCAATCTTGc                                                                                                                    <  4:267565/149‑1 (MQ=255)
                           tCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGACCGGTTTCTGCGTTGCGACCAATCTTGc                                                                                                                    <  4:267567/149‑1 (MQ=255)
                            cGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCg                                                                                                                   <  2:255603/149‑1 (MQ=255)
                             ggCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc                                                                                                                    >  3:10336/1‑147 (MQ=255)
                               ccTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTg                                                                                                                     <  4:212713/144‑1 (MQ=255)
                               ccTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTg                                                                                                                     >  3:212713/1‑144 (MQ=255)
                               ccTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCgaga                                                                                                                <  2:131389/149‑1 (MQ=255)
                                            ttGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTc                                                                                                   >  3:192951/1‑149 (MQ=255)
                                              gCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCcg                                                                                                 >  8:297560/1‑149 (MQ=255)
                                                          gccagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGt                                                                                     <  8:213941/149‑1 (MQ=255)
                                                                    acTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCtttt                                                                           >  4:270893/1‑149 (MQ=255)
                                                                      tAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCtttttt                                                                         >  1:251372/1‑149 (MQ=255)
                                                                      tAGCTACGGGGGTGGTACAGCGTTTCGACAATGTGGGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCtttttt                                                                         <  7:139306/149‑1 (MQ=255)
                                                                        gCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCtttttt                                                                         <  2:79058/147‑1 (MQ=255)
                                                                        gCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTga                                                                       <  7:297560/149‑1 (MQ=255)
                                                                        gCTACGGGGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCtttttt                                                                         <  5:194906/147‑1 (MQ=255)
                                                                           aCGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGagag                                                                    >  2:145711/1‑149 (MQ=255)
                                                                            cGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGagag                                                                    <  3:239545/148‑1 (MQ=255)
                                                                              gtgggtgTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGc                                                                                                             <  7:37024/105‑1 (MQ=255)
                                                                              gtgggtgTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGc                                                                                                             >  8:37024/1‑105 (MQ=255)
                                                                              gtgggtgTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTc                                                                 >  2:48074/1‑149 (MQ=255)
                                                                                        aGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCtt                                                         >  1:125518/1‑147 (MQ=255)
                                                                                         gCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTa                                                      <  8:29621/149‑1 (MQ=255)
                                                                                           gTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAcc                                                    <  8:66874/149‑1 (MQ=255)
                                                                                           gTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAcc                                                    >  7:66874/1‑149 (MQ=255)
                                                                                            tttCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCg                                                   <  1:145711/149‑1 (MQ=255)
                                                                                                gACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCt                                               >  4:73905/1‑149 (MQ=255)
                                                                                                gACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCt                                               >  3:104933/1‑149 (MQ=255)
                                                                                                  cAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                             <  5:219404/149‑1 (MQ=255)
                                                                                                   aaTGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                             >  3:88742/1‑148 (MQ=255)
                                                                                                   aaTGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                             <  4:88742/148‑1 (MQ=255)
                                                                                                   aaTGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCg                                            >  3:294968/1‑149 (MQ=255)
                                                                                                         tgtCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACa                                      <  4:10336/149‑1 (MQ=255)
                                                                                                            cTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTg                                   <  4:192951/149‑1 (MQ=255)
                                                                                                             ttGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGc                                                                                       >  4:224409/1‑96 (MQ=255)
                                                                                                             ttGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTAc                                                                                   >  7:150775/1‑100 (MQ=255)
                                                                                                             ttGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTAc                                                                                   <  8:150775/100‑1 (MQ=255)
                                                                                                             ttGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                             >  2:212646/1‑138 (MQ=255)
                                                                                                             ttGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                             <  1:212646/138‑1 (MQ=255)
                                                                                                             ttGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                                  >  5:266168/1‑149 (MQ=255)
                                                                                                               ggggTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                                  <  3:270893/147‑1 (MQ=255)
                                                                                                               ggggTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                                  <  3:73905/147‑1 (MQ=255)
                                                                                                               ggggTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                                  <  8:77402/147‑1 (MQ=255)
                                                                                                                   tGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAg                            <  2:251372/149‑1 (MQ=255)
                                                                                                                              cttttCAACCGAGGTGAAACGGCCGGATTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCTCCATGTGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtc                 <  7:284449/146‑1 (MQ=255)
                                                                                                                               tGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGCCCAATCTTGCGAGATGCCAGTTTTATCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtct                >  7:31070/1‑149 (MQ=255)
                                                                                                                               tGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAgg                           >  3:133885/1‑138 (MQ=255)
                                                                                                                               tGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAgg                           <  4:133885/138‑1 (MQ=255)
                                                                                                                                gTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtctc               >  6:224645/1‑149 (MQ=255)
                                                                                                                                 ttCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTctct              >  2:201162/1‑149 (MQ=255)
                                                                                                                                    cACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAg           <  1:48074/149‑1 (MQ=255)
                                                                                                                                              aaaCGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATa  >  3:138469/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATA  >  NC_002947/1352157‑1352446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑