Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,413,433	T→A	5.7%	N155I (AAC→ATC)	PP_1235 ←	AhpC/TSA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,413,433	0	T	A	5.7%	209.4 / 2.5	70	N155I (AAC→ATC)	PP_1235	AhpC/TSA family protein
Reads supporting (aligned to +/- strand):  ref base T (35/31);  new base A (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

GGGTTTTGGGGGATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCTTCTTCAGC  >  NC_002947/1413286‑1413579                                                                                                                                                      |                                                                                                                                                   gggTTTTGGGGGATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGtt                                                                                                                                                   <  5:123709/149‑1 (MQ=255)          gggATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGAACAAGGCtt                                                                                                                                           >  2:1771/1‑148 (MQ=255)            gATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTggg                                                                                                                                        >  1:256103/1‑149 (MQ=255)             atatTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGggc                                                                                                                                       >  5:298192/1‑149 (MQ=255)             atatTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGAACAAGGCTTgaaa                                                                                                                                       <  1:1771/149‑4 (MQ=255)                      gCCATCCCGCTGGCGACGCTGATCAAGGCGCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGgccgcc                                                                                                                                >  1:24130/1‑148 (MQ=255)                         aTCCCGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACGGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATAc                                                                                                                           <  2:95676/149‑1 (MQ=255)                            ccGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACgg                                                                                                                         >  2:276859/1‑148 (MQ=255)                              gatGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGTAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCa                                                                                                                       <  4:181666/146‑1 (MQ=255)                               cTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATc                                                                                                                     <  2:256103/149‑1 (MQ=255)                                       gCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGcc                                                                                                             >  6:222407/1‑149 (MQ=255)                                          gATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCgg                                                                                                          >  5:260451/1‑149 (MQ=255)                                               aGGC‑CGTGCGGGAGGCGTGGCCCAAGCAGGAGCCTAGCGTTTCCCCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACt                                                                                                     <  7:240685/149‑1 (MQ=255)                                                ggC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACtt                                                                                                    >  5:118077/1‑149 (MQ=255)                                                ggC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCAGCCAATACGGCATCCACATGCCAGGTCACtt                                                                                                    >  4:296565/1‑149 (MQ=255)                                                 gC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACttt                                                                                                   >  4:78266/1‑149 (MQ=255)                                                  c‑cGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTc                                                                                                  <  5:204276/149‑1 (MQ=255)                                                        cTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAATTTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTcacgc                                                                                              >  1:87263/1‑149 (MQ=255)                                                        cTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGAACAAGGCTTgaa                                                                                                                                        <  5:147899/106‑3 (MQ=255)                                                        cTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGAACAAGGCTTgaa                                                                                                                                        >  6:147899/1‑104 (MQ=255)                                                          ggATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTcacgccac                                                                                           <  6:298192/149‑1 (MQ=255)                                                                    cccAAGCAGGAGCCTAGCGTTTCACCAATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCgg                                                                                                          >  6:317822/1‑124 (MQ=255)                                                                    cccAAGCAGGAGCCTAGCGTTTCACCAATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCgg                                                                                                          <  5:317822/124‑1 (MQ=255)                                                                     ccAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTgg                                                                                >  3:63402/1‑149 (MQ=255)                                                                      cAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTc                                                                                    >  1:309337/1‑144 (MQ=255)                                                                      cAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTc                                                                                    <  2:309337/144‑1 (MQ=255)                                                                      cAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGgc                                                                               <  5:222407/149‑1 (MQ=255)                                                                      cAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGgc                                                                               <  1:276859/149‑1 (MQ=255)                                                                            ggAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCCAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCgcagca                                                                         >  8:252133/1‑149 (MQ=255)                                                                             gAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCAGTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTACCGCCACGCCATTCCTGGCGCAGCAc                                                                        >  4:87062/1‑149 (MQ=255)                                                                                  tAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGTTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTg                                                                                 <  8:96108/135‑1 (MQ=255)                                                                                  tAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGTTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTg                                                                                 >  7:96108/1‑135 (MQ=255)                                                                                    gCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTcc                                                                 <  3:296565/149‑1 (MQ=255)                                                                                        atggccGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGt                                                             <  5:210192/145‑1 (MQ=255)                                                                                           aCCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTcc                                                                 <  2:53885/142‑1 (MQ=255)                                                                                           aCCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTcc                                                                 >  1:53885/1‑142 (MQ=255)                                                                                            ccGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGAt                                                         <  3:78266/149‑1 (MQ=255)                                                                                             cGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGAt                                                         >  6:143485/1‑148 (MQ=255)                                                                                                       aaTGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgc                                              >  7:125686/1‑149 (MQ=255)                                                                                                        aTGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgct                                             <  6:260451/149‑1 (MQ=255)                                                                                                         tGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctg                                            >  4:247282/1‑149 (MQ=255)                                                                                                         tGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctg                                            <  1:41977/149‑1 (MQ=255)                                                                                                         tGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctg                                            >  3:59162/1‑149 (MQ=255)                                                                                                         tGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctg                                            <  2:24130/149‑1 (MQ=255)                                                                                                         aattgaatGGGGCTGCCTTGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgct                                             >  6:60773/8‑149 (MQ=255)                                                                                                          gTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctgc                                           >  3:193701/1‑149 (MQ=255)                                                                                                            aa‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCgg                                         >  2:261633/1‑149 (MQ=255)                                                                                                             gaatGGGGCTGCCTTGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCgg                                         >  2:251000/4‑149 (MQ=255)                                                                                                                   ggCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgct                                             <  3:157266/139‑1 (MQ=255)                                                                                                                   ggCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACg                                   <  3:247282/149‑1 (MQ=255)                                                                                                                   ggCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGAACCCCGTCCTTGTCGATCAGGAAGGtgct                                             >  4:157266/1‑139 (MQ=255)                                                                                                                     cTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGcc                                 >  1:287152/1‑149 (MQ=255)                                                                                                                        ccaGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGCGCTGCGGTCAACGccc                                <  7:252133/145‑1 (MQ=255)                                                                                                                           ggCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGt                            >  6:125371/1‑148 (MQ=255)                                                                                                                                  ccATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTg                    <  8:125686/149‑1 (MQ=255)                                                                                                                                  ccATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTg                    >  2:36410/1‑149 (MQ=255)                                                                                                                                     ttCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGcc                  >  4:118901/1‑148 (MQ=255)                                                                                                                                        aaTTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGc                                  >  1:186773/1‑129 (MQ=255)                                                                                                                                        aaTTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGc                                  <  2:186773/129‑1 (MQ=255)                                                                                                                                        aaTTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATa               >  7:211362/1‑148 (MQ=255)                                                                                                                                         aTTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACa             <  5:125371/149‑1 (MQ=255)                                                                                                                                          tttCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAg            >  6:260507/1‑149 (MQ=255)                                                                                                                                              aaGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGctt         <  4:193701/148‑1 (MQ=255)                                                                                                                                               aGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCgg                                         <  6:163568/115‑1 (MQ=255)                                                                                                                                               aGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCgg                                         >  5:163568/1‑115 (MQ=255)                                                                                                                                                 ccTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGcttctt      >  4:79327/1‑148 (MQ=255)                                                                                                                                                 ccTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCttcttc     <  3:79327/149‑1 (MQ=255)                                                                                                                                                  cTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCttcttc     <  3:118901/148‑1 (MQ=255)                                                                                                                                                     gTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCTTCTTCAg   <  8:211362/147‑1 (MQ=255)                                                                                                                                                     gTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCTTCTTCAGc  <  1:261633/148‑1 (MQ=255)                                                                                                                                                     gTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCTTCTTCAGc  <  1:251000/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GGGTTTTGGGGGATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGC‑CGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGCGTTTCACCGATGCTTTGAATGTAA‑TTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCTTCTTCAGC  >  NC_002947/1413286‑1413579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑