Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,416,943 G→T 7.5% G64V (GGG→GTG)  PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,416,9430GT 7.5% 162.6 / 3.4 53G64V (GGG→GTG) PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base T (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

ACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAA  >  NC_002947/1416795‑1417080
                                                                                                                                                    |                                                                                                                                          
aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATgg                                                                                                                                            <  6:160853/149‑1 (MQ=255)
           cAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATggg‑gg                                                                                                                                        <  1:99607/141‑2 (MQ=255)
           cAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑Gt                                                                                                                                        >  2:99607/1‑141 (MQ=255)
                gtgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCa                                                                                                                           >  3:114026/1‑149 (MQ=255)
                  gACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACAAGCCGACCCCATGCACGTG‑GTCGGCATgttcgtgg                                                                                                                          >  8:50348/1‑141 (MQ=38)
                  gACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACAAGCCGACCCCATGCACGTG‑GTCGGCATgttcgtgg                                                                                                                          <  7:50348/148‑8 (MQ=38)
                     acGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACggtg                                                                                                                      >  5:279342/1‑147 (MQ=255)
                         tCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTa                                                                                                                   >  3:35867/1‑148 (MQ=255)
                            tCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAAt                                                                                                               >  7:211612/1‑149 (MQ=255)
                            tCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAAt                                                                                                               <  2:272321/149‑1 (MQ=255)
                               tGGTCGATTGCCGCTTATCCCTGCCTGAAACCGAGCGGCGCCTGGCTCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTTCAGTGCTGGTCACCCACGAACATGCCGACCAAGAGCATGGG‑GTCGGCTTGCTGTCACGGAGCTACAAtct                                                                                                             >  8:233096/1‑146 (MQ=255)
                                 gTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTAc                                                                                                           <  8:211612/148‑1 (MQ=255)
                                         cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTAcc                                                                                                  >  8:137350/1‑149 (MQ=255)
                                            cTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                                                                               <  4:114026/149‑1 (MQ=255)
                                            cTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                                                                               <  3:52856/149‑1 (MQ=255)
                                                  ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                                                                                         >  1:231171/1‑133 (MQ=255)
                                                  ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                                                                                         <  2:231171/133‑1 (MQ=255)
                                                  ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAg                                                                                         <  3:300098/149‑1 (MQ=255)
                                                   cTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                                                                                        <  6:279342/149‑1 (MQ=255)
                                                   cTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                                                                                        >  6:185434/1‑149 (MQ=255)
                                                       gTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACt                                                                                    >  5:22501/1‑149 (MQ=255)
                                                          aaaCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTg                                                                                 >  6:172390/1‑149 (MQ=255)
                                                              cGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGCGTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                                                                                         <  1:42323/122‑1 (MQ=255)
                                                              cGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGAGTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                                                                                         <  1:37500/122‑1 (MQ=255)
                                                              cGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                                                                                         >  2:42323/1‑121 (MQ=255)
                                                              cGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                                                                                         >  2:37500/1‑121 (MQ=255)
                                                                  cggcgCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCAt                                                                         <  5:185434/149‑1 (MQ=255)
                                                                    gcgcCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGa                                                                                      <  5:200485/134‑1 (MQ=255)
                                                                    gcgcCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGa                                                                                      >  6:200485/1‑134 (MQ=255)
                                                                       ccTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATgcgc                                                                     >  4:73657/1‑148 (MQ=255)
                                                                        cTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCaa                                                                   <  4:35867/149‑1 (MQ=255)
                                                                               tgctCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTg                                                            <  2:268184/149‑1 (MQ=255)
                                                                                 ctCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTg                                                            >  5:268455/1‑147 (MQ=255)
                                                                                    ggggTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAgg                                                        <  6:268455/148‑1 (MQ=255)
                                                                                             gCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAg                                                         >  1:139690/1‑138 (MQ=255)
                                                                                             gCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAg                                                         <  2:139690/138‑1 (MQ=255)
                                                                                              cagctcagctCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGtt                                               <  3:73657/147‑1 (MQ=255)
                                                                                               agctcagctcAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTtc                                            >  7:242874/1‑149 (MQ=255)
                                                                                                    agctcagTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTcgc                                        <  8:242874/148‑1 (MQ=255)
                                                                                                       tcagTGCAGTGCTGGTCAACCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTATCGCgtgt                                    >  3:16580/1‑149 (MQ=255)
                                                                                                          gtgcagtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGc                                 >  5:100558/1‑149 (MQ=255)
                                                                                                           tgcagtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGc                                 >  3:234304/1‑148 (MQ=255)
                                                                                                                     gTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTg                        <  5:172390/147‑1 (MQ=255)
                                                                                                                        aCCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCATCCAAGGGACTTTGCGCTGAATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCata                    <  7:233096/148‑1 (MQ=255)
                                                                                                                          ccACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGcc                                <  4:152204/134‑1 (MQ=255)
                                                                                                                          ccACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGcc                                >  3:152204/1‑134 (MQ=255)
                                                                                                                           cACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCAGGTGGAGCTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATAACgg                >  7:108784/1‑149 (MQ=255)
                                                                                                                            aCGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGTCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCTTGTGGCCAAAGACTGCATATCgg                >  5:64146/1‑148 (MQ=255)
                                                                                                                                gcaagCCGACCCCATGCACGTG‑GTCGGCATGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGa                                                          <  7:26834/98‑1 (MQ=37)
                                                                                                                                gcaagCCGACCCCATGCACGTG‑GTCGGCATGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGa                                                          >  8:26834/5‑102 (MQ=37)
                                                                                                                                 cATGCCGGCCACGTGCGTGGG‑GTCGGCTTGCTGTCACGGCGCTACAGTGTTGCGGTCTAGCTCAGCCAAGGGGCTTTGCGCGGCATGGGCGGGGCGGTGGAGGTGGCCGGGTTTCTCGGGTGTGGCCAGCGCGTGCAGATCggggggct          >  8:257816/1‑143 (MQ=255)
                                                                                                                                     ccGTCCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGaa      <  4:179112/149‑1 (MQ=255)
                                                                                                                                     ccGACCCCGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGaa      <  2:178072/149‑1 (MQ=255)
                                                                                                                                     ccGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGaa      <  7:137350/149‑1 (MQ=255)
                                                                                                                                         ccACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTaa  >  7:54120/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
ACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGG‑GTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAA  >  NC_002947/1416795‑1417080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑