Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,434,165 C→T 6.3% L73L (CTG→CTA)  PP_1255 ← cis‑4‑hydroxy‑D‑proline oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,434,1650CT 6.3% 189.7 / 2.1 63L73L (CTG→CTA) PP_1255cis‑4‑hydroxy‑D‑proline oxidase
Reads supporting (aligned to +/- strand):  ref base C (31/28);  new base T (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GCCCAGGCTGCCTTCGTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGC  >  NC_002947/1434018‑1434308
                                                                                                                                                   |                                                                                                                                               
gcCCAGGCTGCCTTCGTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGagca                                                                                                                                                <  1:159281/149‑1 (MQ=255)
    aGGCTGCCTTCGTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCAt                                                                                                                                            <  3:199493/149‑1 (MQ=255)
          ccTTCGTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGagg                                                                                                                                      <  3:175228/149‑1 (MQ=255)
          ccTTCGTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGa                                                                                                                                        <  6:62908/147‑1 (MQ=255)
            ttCGTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGagg                                                                                                                                      >  2:169231/1‑147 (MQ=255)
               gTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGaggcaggc                                                                                                                                 >  4:228846/1‑149 (MQ=255)
               gTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGaggcaggc                                                                                                                                 >  4:279312/1‑149 (MQ=255)
               gTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGaggcagg                                                                                                                                  <  3:100819/148‑1 (MQ=255)
                   aGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCtt                                                                                                                             >  1:310441/1‑149 (MQ=255)
                   aGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCtt                                                                                                                             >  2:150747/1‑149 (MQ=255)
                    gCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTg                                                                                                                            >  4:8456/1‑149 (MQ=255)
                          ggATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATc                                                                                                                      >  8:275923/1‑149 (MQ=255)
                            aTGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGa                                                                                                                    <  3:219839/149‑1 (MQ=255)
                             tGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGAc                                                                                                                   <  3:229998/149‑1 (MQ=255)
                               cccGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAg                                                                                                                 <  2:310441/149‑1 (MQ=255)
                               cccGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAg                                                                                                                 <  3:8456/149‑1 (MQ=255)
                               cccGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAg                                                                                                                 >  7:51856/1‑149 (MQ=255)
                                ccGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAgg                                                                                                                <  7:275923/149‑1 (MQ=255)
                                ccGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAgg                                                                                                                >  2:111031/1‑149 (MQ=255)
                                ccGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAgg                                                                                                                >  1:159178/1‑149 (MQ=255)
                                        ctgcgctgGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAAt                                                                                                        <  1:169231/149‑1 (MQ=255)
                                           cgctgGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCgg                                                                                                     <  2:159178/149‑1 (MQ=255)
                                           cgctgGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCg                                                                                                      <  5:235496/148‑1 (MQ=255)
                                                       gcCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGc                                                                                                                                          >  2:109130/1‑100 (MQ=255)
                                                       gcCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGc                                                                                                                                          <  1:109130/100‑1 (MQ=255)
                                                          gggCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGt                                                                                      <  1:268798/149‑1 (MQ=255)
                                                           ggCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGtt                                                                                     <  1:150747/149‑1 (MQ=255)
                                                             cgcAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGc                                                                                   <  7:294310/149‑1 (MQ=255)
                                                                    tGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGc                                                                            >  5:295809/1‑149 (MQ=255)
                                                                       gcagcagcCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGCGCCCg                                                                         >  7:168956/1‑149 (MQ=255)
                                                                        cagcagcCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCgg                                                                        >  3:39028/1‑149 (MQ=255)
                                                                          gcagcCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCa                                                                      <  1:111031/149‑1 (MQ=255)
                                                                             gcCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGCGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTg                                                                   >  4:289193/1‑149 (MQ=255)
                                                                                        cACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGAcgc                                                        >  3:138190/1‑149 (MQ=255)
                                                                                              aTGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAg                                                  >  5:144863/1‑149 (MQ=255)
                                                                                                ggCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGc                                                >  8:277954/1‑149 (MQ=255)
                                                                                                  ccTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGATGCAGGCGCTTTAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCg                                              <  6:118901/149‑1 (MQ=255)
                                                                                                   cTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGcccggcctgg                                             >  1:153453/1‑142 (MQ=255)
                                                                                                   cTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCgg                                             >  5:271175/1‑149 (MQ=255)
                                                                                                         cAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGTAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCt                                       >  6:21237/1‑149 (MQ=255)
                                                                                                         cAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGTAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCt                                       >  4:55154/1‑149 (MQ=255)
                                                                                                         cAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCGGCCTGGgcgcgt                                       <  3:279312/149‑3 (MQ=255)
                                                                                                          aGGTACTTCCAGTCCAGGCGCTGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGaa                                                              <  4:83684/125‑1 (MQ=255)
                                                                                                          aGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGTCATTGCCGAAGGACGCGACCAGGCAGGGCGCCTg                                      >  1:171140/1‑149 (MQ=255)
                                                                                                          aGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGaa                                                              >  3:83684/1‑125 (MQ=255)
                                                                                                          aGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTg                                      >  5:200097/1‑149 (MQ=255)
                                                                                                            gTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGcc                                                     <  6:40265/132‑1 (MQ=255)
                                                                                                            gTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCACGGCATTGCCGAAGGACGCGcc                                                     >  5:40265/1‑132 (MQ=255)
                                                                                                             tACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCg                                   >  3:11254/1‑149 (MQ=255)
                                                                                                             tACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCg                                   <  4:11254/149‑1 (MQ=255)
                                                                                                              aCTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCgg                                  <  3:228846/149‑1 (MQ=255)
                                                                                                               cTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCGGCCTGGgcgcgtccttc                                  <  2:153453/148‑8 (MQ=255)
                                                                                                                  ccagtccagGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCgg                                                                        >  3:292588/1‑107 (MQ=255)
                                                                                                                     gtccagGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGAc                             >  7:130992/1‑147 (MQ=255)
                                                                                                                          ggCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCa                                                                      >  7:60975/1‑101 (MQ=255)
                                                                                                                          ggCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCa                                                                      <  8:60975/101‑1 (MQ=255)
                                                                                                                                gCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGa                <  6:295809/149‑1 (MQ=255)
                                                                                                                                gCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGa                >  5:302500/1‑149 (MQ=255)
                                                                                                                                       cATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCt         <  8:51856/149‑1 (MQ=255)
                                                                                                                                           gggTCGAGTAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCg     <  5:21237/149‑1 (MQ=255)
                                                                                                                                           gggTCGAGTAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCg     <  3:55154/149‑1 (MQ=255)
                                                                                                                                            ggTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCgg    <  7:277954/149‑1 (MQ=255)
                                                                                                                                             gTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCggg   >  8:181639/1‑149 (MQ=255)
                                                                                                                                               cGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGc  >  4:201359/1‑148 (MQ=255)
                                                                                                                                                  gcagcaTGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATg              >  7:67043/1‑133 (MQ=255)
                                                                                                                                                  gcagcaTGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATg              <  8:67043/133‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GCCCAGGCTGCCTTCGTTCAGCGTGCGGATGCCCGCCACGCTGCGCTGGAACGGCGCCGGGCGCAGGTTGAGCAGCAGCCGGGTGAACCACGGCATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCCGGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGC  >  NC_002947/1434018‑1434308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑