Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,464,198 T→A 8.9% T474S (ACC→TCC) ‡ algX ← alginate biosynthesis protein AlgX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,464,1980TA 8.9% 100.8 / 3.2 45T474S (ACC→TCC) ‡algXalginate biosynthesis protein AlgX
Reads supporting (aligned to +/- strand):  ref base T (27/12);  new base A (2/2);  total (29/16)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

TTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCG  >  NC_002947/1464051‑1464346
                                                                                                                                                   |                                                                                                                                                    
ttGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTc                                                                                                                                                     >  5:204932/1‑149 (MQ=255)
    gCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTgcg                                                                                                                                                 >  5:167824/1‑149 (MQ=255)
    gCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCGgcg                                                                                                                                                 >  5:172191/1‑149 (MQ=255)
    gCTTTTCGATCCCCTGGTAGTAACCATGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATGCGTTTGAAAAGGGTCATGGGTCGCCTCCGTGGCCTGCCTGCGCGGTCTgcg                                                                                                                                                 >  4:59242/1‑149 (MQ=255)
           gATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGAGCGGCACCACCAAACAAGTCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACCGCGCAGGCa                                                                                                                                           <  2:52208/148‑1 (MQ=255)
            aTCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACCGCGCAGGCag                                                                                                                                          >  5:74364/1‑147 (MQ=255)
            aTCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACCGCGCAGGCag                                                                                                                                          <  6:74364/148‑2 (MQ=255)
              ccccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGc                                                                                                                                       >  3:137698/1‑149 (MQ=255)
                ccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCg                                                                                                                                      >  6:273269/1‑148 (MQ=255)
                    gtagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAgtt                                                                                                                                 >  2:28245/1‑149 (MQ=255)
                        taACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGttgttg                                                                                                                             >  3:138251/1‑149 (MQ=255)
                            ccTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAg                                                                                                                                   >  7:281784/1‑139 (MQ=255)
                                gggcggTACCAGGGGGGGGTGCGCGCAACCACAAAACATGCCCAACGCTAGCAGGGCGGGGGTGTAAATTCGTTTCAAAAGGGTAATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCTGGCACGGCGAAGTTGTTGCGTTTGCa                                                                                                                     <  8:134114/148‑1 (MQ=255)
                                 ggcgtTACCCGCGCGGCTGGCGCGCAACAACCAAACACGGCCAACGCGAGCAGGGGGGGGGTTTAAATTCTTTTTAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCag                                                                                                                    <  6:167824/149‑1 (MQ=255)
                                 ggcggAACCCGGGCGGCGTGCGCGGAACCACAAAACAAGCCCACCGCGCGCCGGGGGGGGCTGTAAATTCGTTTTAAACGGGTAATGGGGCGCCTCACGGGCGTGCCTGCGCGGTCTGCGCTGGCACGGCGAAGTTGTTGCGTTTGCag                                                                                                                    <  6:172191/149‑1 (MQ=255)
                                      tACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGt                                                                                                                                                      >  6:67996/1‑110 (MQ=255)
                                      tACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGt                                                                                                                                                      <  5:67996/110‑1 (MQ=255)
                                         cAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGc                                                                                                            >  4:127303/1‑149 (MQ=255)
                                               ggcgGGGGCGGAACCACAAAACATGGCCACCCGGTGGTGGGCGGGGGTGTAATTTGTTTTTAAATGGGTTTTGGGGGGCCTCACGGGCCTGCGGGGGGGGTGGGGCCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGAc                                                                                                      <  4:137698/149‑1 (MQ=255)
                                                          caccacCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgtt                                                                                                                              >  5:326633/1‑114 (MQ=255)
                                                               ccaAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCCCTGGTCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGCCCTTCTGGGTGACGCtt                                                                                      >  7:149259/1‑149 (MQ=255)
                                                               ccaAACAAGGCCAACGCGAGCAGGCCGGGGCTGGAAATTCGTTTGAAAAGCGTCATGGCTCGCCTCACTGGCCTTCCTCCGCGGTCTGCGAAGGCACGGCGAAGTTGATCAGTTTGCAGAGCTTGGCTTCGACCTTCTGCGTGCCGCtt                                                                                      >  7:91922/1‑149 (MQ=255)
                                                                     aaGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGc                                                                                >  8:18082/1‑149 (MQ=255)
                                                                       ggCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTATGCGCAGGCACGGCGAAGTTTTTTGGTTTTCAGAGCTTGGCTTCGCCCTTCTGGGTGACGCTTTCCGGacc                                                                              >  8:281161/1‑149 (MQ=255)
                                                                                                    ttCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTgg                                                                                               >  3:121830/1‑103 (MQ=255)
                                                                                                          tGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTTTACCTCGAACGCGAGCAGGCGCTGGCTGGcc                                            <  1:28245/148‑1 (MQ=255)
                                                                                                            aaaaGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGTGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAg                                         >  7:156777/1‑149 (MQ=255)
                                                                                                            aaaaGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCa                                          <  7:18082/148‑1 (MQ=255)
                                                                                                                       gggTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTc                              >  2:67026/1‑149 (MQ=255)
                                                                                                                         gTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTc                                       <  5:227226/138‑1 (MQ=255)
                                                                                                                         gTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTc                                       >  6:227226/1‑138 (MQ=255)
                                                                                                                         gTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTcgc                            >  7:280038/1‑149 (MQ=255)
                                                                                                                             ccTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCAt                        >  4:50725/1‑149 (MQ=255)
                                                                                                                              cTCACGGCCCGGCGTGCGCGGGGTCCCCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATc                       <  6:204932/149‑1 (MQ=255)
                                                                                                                               tCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGc                                                           >  1:215194/1‑112 (MQ=255)
                                                                                                                               tCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGc                                                           <  2:215194/112‑1 (MQ=255)
                                                                                                                                 aCTGGCCTGCCTGCGCGGGCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTgg                    <  3:59242/149‑1 (MQ=255)
                                                                                                                                       ctgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTACTGGGTGCCGCTTTACTGCACATGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc               >  7:200351/1‑148 (MQ=255)
                                                                                                                                        tgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGaaacctc             >  8:190585/1‑144 (MQ=255)
                                                                                                                                        tgcctgcGCAGACCGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCa                                                          >  3:116693/1‑104 (MQ=255)
                                                                                                                                          cctgcGCTGTGTGCGCTGGCACGGCGAAGTTGTTGTGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGaa            <  8:156777/148‑1 (MQ=255)
                                                                                                                                            tgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCg         >  1:155940/1‑149 (MQ=255)
                                                                                                                                             gcgcGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAgg                                                        <  4:160889/101‑1 (MQ=255)
                                                                                                                                             gcgcGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAgg                                                        >  3:160889/1‑101 (MQ=255)
                                                                                                                                                 ggTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCgg                                                                                 >  4:168701/1‑72 (MQ=255)
                                                                                                                                                 ggTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCgg                                                                                 <  3:168701/72‑1 (MQ=255)
                                                                                                                                                  gTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTc   <  3:127303/149‑1 (MQ=255)
                                                                                                                                                   tCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCg  >  1:181925/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCG  >  NC_002947/1464051‑1464346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑