Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,491,218 A→T 8.7% E294V (GAA→GTA)  cysNC → sulfate adenylyltransferase subunit 1/adenylyl‑sulfate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,491,2180AT 8.7% 130.5 / 3.2 46E294V (GAA→GTA) cysNCsulfate adenylyltransferase subunit 1/adenylyl‑sulfate kinase
Reads supporting (aligned to +/- strand):  ref base A (24/18);  new base T (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CAGTACGTCAACCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTGGTGTGGATGG  >  NC_002947/1491082‑1491364
                                                                                                                                         |                                                                                                                                                  
cAGTACGTCAACCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTgg                                                                                                                                         >  8:166575/1‑148 (MQ=255)
 aGTACGTCAACCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGa                                                                                                                                        >  3:307139/1‑148 (MQ=255)
  gTACGTCAACCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTTCGTAGGTGacgat                                                                                                                                          <  1:307682/145‑5 (MQ=255)
  gTACGTCAACCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGACAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTTCGTAGGTGacgat                                                                                                                                          >  2:307682/1‑141 (MQ=255)
   tACGTCAACCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGaaaa                                                                                                                                     >  1:281173/1‑149 (MQ=255)
     cGTCAACCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACg                                                                                                                                   <  1:136594/149‑1 (MQ=255)
         aaCCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTgg                                                                                                                               >  4:229362/1‑149 (MQ=255)
          aCCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAACCGCTGGc                                                                                                                              >  2:241308/1‑149 (MQ=255)
             gTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCcca                                                                                                                           <  7:166575/149‑1 (MQ=255)
                ccAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCccaggc                                                                                                                        >  6:276282/1‑149 (MQ=255)
                ccAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCccagg                                                                                                                         <  1:226247/148‑1 (MQ=255)
                    ccTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCccaggccagg                                                                                                                    >  3:185238/1‑149 (MQ=255)
                                        cgGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATgg                                                                                                  >  5:4552/1‑147 (MQ=255)
                                        cgGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATgg                                                                                                  >  2:88476/1‑147 (MQ=255)
                                           gCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGAc                                                                                             <  6:143579/149‑1 (MQ=255)
                                           gCACCA‑CGCCACTGGCGATGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGa                                                                                                 <  2:16261/145‑1 (MQ=255)
                                           gCACCA‑CGCCACTGGCGATGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGa                                                                                                 >  1:16261/1‑145 (MQ=255)
                                            cACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCTTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGAc                                                                                             >  2:149800/1‑148 (MQ=255)
                                            cACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACg                                                                                            <  4:307139/149‑1 (MQ=255)
                                             aCCATCGCGCGTGGCG‑TGGTGCACAATGGTGACGCGTTTGTCGTGGTGCCCGGGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCAGCTACGAAGGTCAACTGGAAAACGCTGGGCCAGGCCAGTCCGTgt                                                                                                              <  8:274491/130‑2 (MQ=255)
                                                    ccAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGAc                                                                                    <  3:229362/149‑1 (MQ=255)
                                                     cAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCTTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACa                                                                                   <  1:149800/149‑1 (MQ=255)
                                                                gtgCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTg                                                                          <  5:276282/148‑1 (MQ=255)
                                                                 tgCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATg                                                                                                   >  3:83357/1‑122 (MQ=255)
                                                                 tgCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAAGCCAGGCCGTGACCCTGACCATg                                                                                                   <  4:83357/122‑1 (MQ=255)
                                                                    acaAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCGATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCTTGGAAGACGAGATCGACATCTCCCGTGGCGAc                                                                     >  2:265264/1‑149 (MQ=255)
                                                                       aaGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACctg                                                                  >  1:212707/1‑149 (MQ=255)
                                                                          gTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTgg                                                                         >  1:74446/1‑139 (MQ=255)
                                                                          gTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCCACATCTCCCGTgg                                                                         <  2:74446/139‑1 (MQ=255)
                                                                               gagaTTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACa                                                          >  8:215306/1‑149 (MQ=255)
                                                                                 gaTTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATg                                                        >  6:3461/1‑149 (MQ=255)
                                                                                  aTTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGCCCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGc                                                       >  2:256893/1‑149 (MQ=255)
                                                                                     gTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCg                                                     <  5:3461/148‑1 (MQ=255)
                                                                                      tCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGa                                                    >  4:82456/1‑148 (MQ=255)
                                                                                      tCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGa                                                    >  2:50650/1‑148 (MQ=255)
                                                                                                gTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCccgc                                         <  1:88476/149‑1 (MQ=255)
                                                                                                               ccGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGt                          >  5:304284/1‑149 (MQ=255)
                                                                                                                cGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGtt                         <  3:82456/149‑1 (MQ=255)
                                                                                                                        gTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCa                 >  4:153961/1‑149 (MQ=255)
                                                                                                                             agttCACCTTCGTAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTg            >  3:28241/4‑149 (MQ=255)
                                                                                                                               gTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTGgt          <  6:304284/149‑1 (MQ=255)
                                                                                                                                  aCCTTCGTAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTGgtgt        <  4:28241/148‑1 (MQ=255)
                                                                                                                                    cTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTGgtgt        <  7:168450/146‑1 (MQ=255)
                                                                                                                                    cTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTGgtgt        >  8:168450/1‑146 (MQ=255)
                                                                                                                                       cGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTGGTGTGGATgg  >  5:123776/1‑149 (MQ=255)
                                                                                                                                       cGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTGGTGTGGATg   <  7:215306/148‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
CAGTACGTCAACCGTCCCAACCTGAACTTCCGCGGCTTCGCGGGCACCATCGCCAGTGGCG‑TGGTGCACAAAGGTGACGAGATTGTCGTGCTGCCGTCGGGCAAGAGCAGCCGGGTCAAGTCCATCGTCACCTACGAAGGTGAACTGGAAAACGCTGGCCCAGGCCAGGCCGTGACCCTGACCATGGAAGACGAGATCGACATCTCCCGTGGCGACCTGCTGGTACATGCCGACAACGTCCCGCCGGTCACCGACCAGTTCGACGCCATGCTGGTGTGGATGG  >  NC_002947/1491082‑1491364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑