| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 197,089 | T→C | 12.7% | N865N (AAT→AAC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 197,089 | 0 | T | C | 12.7% | 630.1 / 69.9 | 262 | N865N (AAT→AAC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (112/114); new base C (17/16); total (132/130) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
Only 100 of 262 total aligned reads displayed.
CGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCT > NC_002947/196942‑197235 | cGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑c < 5:149309/149‑1 (MQ=33) ccAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCTGAAT‑‑C‑CGa < 5:52367/149‑1 (MQ=21) ccAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGACT‑‑C‑CGa > 3:226574/1‑149 (MQ=21) ccAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGa < 6:235422/149‑1 (MQ=34) aaCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGGAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGAccc > 5:129382/1‑149 (MQ=25) aCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCTTCACCAACGAGAACGCCACAGGCGGTAATTTCGAGCAGCTGAACCCGAAT‑‑C‑CGAcccc > 8:54245/1‑149 (MQ=255) ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCg < 5:24176/149‑1 (MQ=37) cATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCgg > 3:125914/1‑149 (MQ=37) ttACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTAGACCGTCAGCGTCAACATCGAGAACGACACAGGCGGTAATTTCGACCAACTGACAAAGAAT‑‑C‑CGACCCAGGCt > 6:286025/1‑149 (MQ=11) cGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGa < 7:293593/149‑1 (MQ=37) cGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGa < 2:215323/149‑1 (MQ=37) ttGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTc > 6:3450/1‑144 (MQ=37) gAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCa < 5:286025/148‑1 (MQ=37) gCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGa < 6:90836/148‑1 (MQ=37) gCATGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAAc < 4:243174/149‑1 (MQ=25) gCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAAc < 6:212900/149‑1 (MQ=37) aGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGAc < 6:145906/149‑1 (MQ=35) aCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTc > 8:134813/1‑149 (MQ=34) aCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTc < 2:227823/149‑1 (MQ=34) aCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTc > 4:310450/1‑149 (MQ=34) ccAGGGGAGCGTCGTTTTCGAGACCGCGTCGAATGACGTCTAGAACTACGGTTCGACCGTCAGCGTCAGCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCgg < 4:178972/125‑1 (MQ=11) ccAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCg < 3:119628/148‑1 (MQ=34) cAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGt > 3:60299/1‑149 (MQ=17) cAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGt < 7:184182/149‑1 (MQ=34) aGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGCTTCGACCGTCAGCGTCACCAACGAGAACGCCACAGCCGGTAATTTACAGCAAATGACCCCGAAA‑‑C‑CGCCCACGGATC‑‑AGACCACCAACAACGACTCGCTc > 4:189461/1‑149 (MQ=11) gTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGcc > 2:26586/1‑147 (MQ=38) tCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACg < 3:189317/125‑1 (MQ=35) tCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGccac > 4:259531/1‑148 (MQ=39) tCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTAGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGccac > 3:45841/1‑148 (MQ=255) gacttCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCcaccac > 8:167351/4‑149 (MQ=255) gATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGccacca < 3:208083/148‑1 (MQ=39) aTTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATATCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAAc < 8:233015/128‑1 (MQ=25) tttCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACcg < 1:218765/149‑1 (MQ=39) ccAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGa > 3:279662/1‑149 (MQ=39) cAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGAc > 3:273168/1‑149 (MQ=21) cAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGa > 3:307232/1‑112 (MQ=35) cAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGAc < 1:114589/149‑1 (MQ=39) aGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATG‑CACCACcg > 4:305089/1‑143 (MQ=255) cGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACg > 4:28977/1‑149 (MQ=39) cGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACg < 4:49069/149‑1 (MQ=39) ggCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTTGAt > 8:373/1‑127 (MQ=2) ggCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTTGAt < 7:373/127‑1 (MQ=2) aTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGc < 4:67706/149‑1 (MQ=37) gACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGccac > 1:134356/1‑125 (MQ=38) ccGTCTACACAAACGGTTCGACCGTCAGCGTCACCATCGAGCACGCCACACGCGGTAATTTCGAGCACCTGACCCCGCAT‑‑C‑CGACACCGGCTC‑‑AGACCCCGCTCAACGACTCGGTCGATGCCACCACCGCGACCCAGACCGCGAGCCCg > 2:89141/2‑149 (MQ=11) aCGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCg > 4:148709/1‑149 (MQ=21) cGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGt > 4:120303/1‑149 (MQ=37) caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATAGCGCGACCCCGTATCGGAGACCACGATCAACGACTCGGACGATACCACCACCGCGACCCTGACGCCGACCCCGTc > 1:98193/1‑149 (MQ=11) caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCa > 3:105513/1‑149 (MQ=37) aacaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGAccc > 8:68971/1‑127 (MQ=25) aacaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGAccc < 7:68971/127‑1 (MQ=25) aacaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCAc < 4:273168/149‑1 (MQ=21) aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCAc < 4:103960/149‑1 (MQ=37) aatacGGTTCGACTGTCAGCGTCACCATCGTGAACGCCACAGGCGGTATTTTCGATCAACTGACCCCTAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGGCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCAcc < 8:288241/146‑1 (MQ=11) caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCg > 4:113022/1‑142 (MQ=37) aacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCg > 2:134653/1‑148 (MQ=37) acGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCg > 2:189722/1‑147 (MQ=37) acGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCg > 5:327252/1‑147 (MQ=37) gACCGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAggcggc < 4:60299/148‑1 (MQ=21) gTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAAt > 7:102860/1‑149 (MQ=255) tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAAt > 7:253043/1‑148 (MQ=39) cAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCa < 5:204232/149‑1 (MQ=39) aGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCAc < 7:123599/149‑1 (MQ=39) cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTGATCAct > 5:112509/1‑147 (MQ=17) cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCt < 7:282354/149‑1 (MQ=38) gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCg > 6:189607/1‑123 (MQ=35) taaCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACACCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACAACGATCAAAGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTa > 2:43049/3‑148 (MQ=255) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTGATCACTTccac > 6:161604/1‑149 (MQ=12) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTGATCACTTaca < 4:160676/148‑1 (MQ=17) ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACAcc > 3:169972/1‑149 (MQ=37) aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAggcggc < 5:268807/132‑1 (MQ=21) aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAggcggc > 6:268807/1‑132 (MQ=21) tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCCCGATCAACGACTCGCTCGATCCCACCACCGCGACACTGACCGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGt > 1:297113/1‑148 (MQ=11) gagaACGCCACAGGCTGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTGATCACTTACACCGTGa < 7:54245/148‑1 (MQ=12) gagaACGCCACAGGCGGTAATTTCGATCAACTGACCGCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGAATGCTAGACCGTCGGATCACCGAAGGCGGCGTAATCACCTACACCGTGa < 1:43049/149‑1 (MQ=11) gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAggcggc > 8:20514/1‑129 (MQ=35) gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAggcggc < 7:20514/129‑1 (MQ=35) agaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCTCCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGAcc < 1:253805/149‑1 (MQ=37) agaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGAcc > 2:249171/1‑149 (MQ=37) aCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTg < 6:112509/149‑1 (MQ=17) cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa < 8:271477/149‑1 (MQ=17) cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGa < 8:224131/149‑1 (MQ=37) gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt > 3:199546/1‑146 (MQ=17) gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa < 4:199546/148‑1 (MQ=17) cacaGGCGGTAATTTCGAGCAACTGACCCCGAAC‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATc > 3:240413/1‑127 (MQ=38) acaGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAg < 1:170286/98‑1 (MQ=37) caGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAAt < 5:93577/149‑1 (MQ=35) gTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCAACGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGcc < 8:253043/149‑1 (MQ=21) ttCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGAc > 1:31735/1‑149 (MQ=34) tCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGAc < 3:64891/148‑1 (MQ=34) aGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg < 2:309321/149‑1 (MQ=34) ccAACTGACCCCGAAT‑‑C‑CGACCGCGGCTA‑‑AGCCCACGATCCACGACGCGGTCGTTGCCACCTCCGCGACCCTTACGGCGAGCCGGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCgg < 2:297113/148‑1 (MQ=11) cAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt < 3:25922/149‑1 (MQ=34) aCCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGCCGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGt > 1:95213/1‑149 (MQ=255) aCCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGAca < 3:292991/147‑2 (MQ=14) ccccGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTg > 3:160610/1‑129 (MQ=35) ccGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAc > 1:289648/1‑149 (MQ=39) cGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCAc < 1:8187/100‑1 (MQ=37) cGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAcc > 3:143711/1‑149 (MQ=39) aaT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCt < 4:81058/149‑1 (MQ=39) | CGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAT‑‑C‑CGACCCCGGCTC‑‑AGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCT > NC_002947/196942‑197235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |