Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,493,639 A→G 6.5% V411A (GTA→GCA)  PP_1306 ← Pyocin S‑type killer domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,493,6390AG 6.5% 177.6 / 2.9 61V411A (GTA→GCA) PP_1306Pyocin S‑type killer domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (26/31);  new base G (2/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

CAAGGGTGCCTACTGCGACCGAAGGGACAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGAGCCTGGAGGTTTTC  >  NC_002947/1493496‑1493784
                                                                                                                                               |                                                                                                                                                 
cAAGGGTGCCTACTGCGACCGAAGGGACAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGc                                                                                                                                               >  3:296227/1‑148 (MQ=255)
 aaGGGTGCCTACTGCGACCGAAGGGACAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGc                                                                                                                                               >  1:165635/1‑147 (MQ=255)
 aaGGGTGCCTACTGCGACCGAAGGGACAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGc                                                                                                                                               >  6:302860/1‑147 (MQ=255)
        ccTACTGCGACCGAAGGGACAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCgcgc                                                                                                                                      <  2:269185/149‑1 (MQ=255)
         cTACTGCGACCGAAGGGACAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCt                                                                                                                                     >  4:296703/1‑149 (MQ=255)
                 aCCGAAGGGACAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGat                                                                                                                             <  4:128370/149‑1 (MQ=255)
                      atggACAGTCGTATCAACAAGCCGCGTAAGTGCCGCAGCGATCGATTCAGCCAAGTCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTCGATACCGCTAGCCGCGCTTCATTGATATAgg                                                                                                                        <  2:242869/147‑1 (MQ=255)
                           cAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGaca                                                                                                                   <  2:165635/149‑1 (MQ=255)
                                gTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTAcg                                                                                                              >  2:228563/1‑149 (MQ=255)
                                 tAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCAtt                                                                                                                                <  2:215305/130‑1 (MQ=255)
                                 tAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCAtt                                                                                                                                >  1:215305/1‑130 (MQ=255)
                                    caacaaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTAcgccg                                                                                                           >  6:235889/1‑148 (MQ=255)
                                     aacaaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATGCCGCTAGCCGCGCTTCATTGa                                                                                                                              <  3:118050/128‑1 (MQ=255)
                                     aacaaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATGCCGCTAGCCGCGCTTCATTGa                                                                                                                              <  3:122664/128‑1 (MQ=255)
                                     aacaaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATGCCGCTAGCCGCGCTTCATTGa                                                                                                                              >  4:118050/1‑128 (MQ=255)
                                     aacaaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATGCCGCTAGCCGCGCTTCATTGa                                                                                                                              >  4:122664/1‑128 (MQ=255)
                                     aacaaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTAcgccgc                                                                                                          >  8:19785/1‑148 (MQ=255)
                                      acaaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCa                                                                                                         >  3:213906/1‑148 (MQ=255)
                                       caaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCaaa                                                                                                       <  3:296703/149‑1 (MQ=255)
                                       caaGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCCAAAGCCGCGCTGCATTGATTTAGGAGCCACTACGCCGCaaa                                                                                                       >  2:111763/1‑149 (MQ=255)
                                          gCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGga                                                                                                                       >  7:222238/1‑130 (MQ=255)
                                          gCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGga                                                                                                                       <  8:222238/130‑1 (MQ=255)
                                              ggTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCtt                                                                                                >  8:96610/1‑149 (MQ=255)
                                               gTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTg                                                                                               >  5:53100/1‑149 (MQ=255)
                                                tAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGc                                                                                              <  5:120549/149‑1 (MQ=255)
                                                    tGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCttt                                                                                          <  4:213906/149‑1 (MQ=255)
                                                    tGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCttt                                                                                          <  1:228563/149‑1 (MQ=255)
                                                     gCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGat                                                                                                                             >  8:287302/1‑113 (MQ=255)
                                                     gCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGat                                                                                                                             <  7:287302/113‑1 (MQ=255)
                                                     gCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTg                                                                                         >  2:238114/1‑149 (MQ=255)
                                                      ccGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCTGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTg                                                                                         <  5:238189/148‑1 (MQ=255)
                                                             gatcgatTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTctg                                                                                 <  1:238114/149‑3 (MQ=255)
                                                                cgatTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCt                                                                               >  5:81840/1‑148 (MQ=255)
                                                                   ttCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTca                                                                            <  6:155555/148‑1 (MQ=255)
                                                                    tCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTcag                                                                           >  5:4667/1‑148 (MQ=255)
                                                                       gCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCagcagc                                                                       >  5:237616/1‑149 (MQ=255)
                                                                        ccAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTc                                                                                   <  2:71350/136‑1 (MQ=255)
                                                                        ccAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTc                                                                                   >  1:71350/1‑136 (MQ=255)
                                                                         cAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGcc                                                                      >  7:1464/1‑148 (MQ=255)
                                                                             gCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCg                                                                 >  6:136417/1‑149 (MQ=255)
                                                                             gCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCACAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCg                                                                 >  6:292689/1‑149 (MQ=255)
                                                                                     aGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGttcggctt                                                         <  5:235889/149‑1 (MQ=255)
                                                                                      gCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGttcggcttc                                                        >  6:76196/1‑149 (MQ=255)
                                                                                              ggCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCctgc                                                 <  8:149558/148‑1 (MQ=255)
                                                                                                           gTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGcttc                                   <  7:96610/149‑1 (MQ=255)
                                                                                                           agggTACCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGcttc                                   <  1:111763/147‑1 (MQ=255)
                                                                                                            tGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGcttct                                  <  8:1464/149‑1 (MQ=255)
                                                                                                              gTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCttcttc                                <  5:136417/149‑1 (MQ=255)
                                                                                                                  ccccAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCtgt                            <  5:302860/149‑1 (MQ=255)
                                                                                                                  ccccAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGACTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCtgt                            <  6:53100/149‑1 (MQ=255)
                                                                                                                      aaTTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAg                        <  6:237616/149‑1 (MQ=255)
                                                                                                                         tGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGc                       >  8:231031/1‑147 (MQ=255)
                                                                                                                             aTTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCtg                             >  4:244410/1‑137 (MQ=255)
                                                                                                                             aTTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCtg                             <  3:244410/137‑1 (MQ=255)
                                                                                                                                 cGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGAGcc             <  7:19785/149‑1 (MQ=255)
                                                                                                                                     aGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGAGCCTGGa         <  5:76196/149‑1 (MQ=255)
                                                                                                                                     aGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCCTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGAGCCTGGa         <  6:81840/149‑1 (MQ=255)
                                                                                                                                     aGGTGTGGATACCGCTAGCCGCGATTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCATCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGTGCCTGGa         <  4:296227/149‑1 (MQ=255)
                                                                                                                                            gATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGAGCCTGGAGGTTTTc  >  2:270959/1‑149 (MQ=255)
                                                                                                                                            gATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGAGCCTGGAGGTTTTc  <  7:231031/149‑1 (MQ=255)
                                                                                                                                            gATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGAGCCTGGAGGTTTTc  <  5:292689/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CAAGGGTGCCTACTGCGACCGAAGGGACAGTCGTAGCAACAAGCCGGGTAAGTGCCGCTGCGATCGATTCAGCCAAGGCAGAGTAAGCCGCTTCGGCTACGGCAAAAGTGGTAGCCCCAATTGGGATTACGGCAGGTGTGGATACCGCTAGCCGCGCTTCATTGATATAGGAGACACTACGCCGCAAAGCCTCTTGCCTTTGGCGCTCGGCTTCAGCAGCCAAGCGTTCGGCTTCGGCCTGCTGGCGTGCAGCTTCTTCCTGTGCAGCGAGTTGAGCCTGGAGGTTTTC  >  NC_002947/1493496‑1493784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑