Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,531,233 C→A 7.7% intergenic (+35/+13) lpxC → / ← PP_1344 UDP‑3‑O‑[3‑hydroxymyristoyl] N‑acetylglucosamine deacetylase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,531,2330CA 7.7% 204.0 / 6.6 65intergenic (+35/+13)lpxC/PP_1344UDP‑3‑O‑[3‑hydroxymyristoyl] N‑acetylglucosamine deacetylase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/26);  new base A (3/2);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.58e-01

AACAACCAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCATGCCCGCCACGCTT  >  NC_002947/1531085‑1531380
                                                                                                                                                     |                                                                                                                                                    
aacaacCAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTc                                                                                                                                                      <  4:271360/149‑1 (MQ=255)
  caacCAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCgg                                                                                                                                                    >  1:152296/1‑149 (MQ=255)
   aacCAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCggg                                                                                                                                                   >  2:178811/1‑149 (MQ=255)
   aacCAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑ATGTTGCGGCAGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCggg                                                                                                                                                   >  2:21514/1‑149 (MQ=255)
     ccAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCggg                                                                                                                                                   <  2:23262/147‑1 (MQ=255)
     ccAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTg                                                                                                                                                 >  2:15791/1‑149 (MQ=255)
     ccAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATCAAGGCCACCTTCGGGTg                                                                                                                                                 >  4:28390/1‑149 (MQ=255)
     ccAGCTGTTGCGCAAGCTGATTGCTGAAAACGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCggg                                                                                                                                                   >  1:23262/1‑147 (MQ=255)
              gcgcAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCttttt                                                                                                                                         <  2:152296/148‑1 (MQ=255)
                        aTTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATgg                                                                                                                                    <  5:6705/143‑1 (MQ=255)
                        aTTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATgg                                                                                                                                    >  6:6705/1‑143 (MQ=255)
                        aTTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCCGAAGGTGGCCttaatgaa                                                                                                                                      <  5:43011/141‑7 (MQ=255)
                        aTTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCCGAAGGTGGCCttaatgaa                                                                                                                                      >  6:43011/1‑135 (MQ=255)
                            cTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTgg                                                                                                                          <  5:108030/149‑1 (MQ=255)
                             tGAAACCGATGCCT‑GGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGc                                                                                                                        >  2:186760/1‑149 (MQ=255)
                               aaaCCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGc                                                                                                                        <  2:173690/148‑1 (MQ=255)
                                     aTGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGgcc                                                                                                                 >  7:233289/1‑149 (MQ=255)
                                         cTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGgccgcc                                                                                                              >  4:137033/1‑148 (MQ=255)
                                          tGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCaa                                                                                                            >  1:269974/1‑149 (MQ=255)
                                                  ggTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTc                                                                                                      >  5:20697/1‑147 (MQ=255)
                                                   gTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCCACTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTc                                                                                                      >  1:70553/1‑147 (MQ=255)
                                                   gTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGgcc                                                                                                                 <  1:124207/135‑1 (MQ=255)
                                                   gTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCa                                                                                                    <  3:137033/148‑1 (MQ=255)
                                                   gTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAg                                                                                                   >  4:178910/1‑149 (MQ=255)
                                                       cTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCg                                                                                               <  2:70553/149‑1 (MQ=255)
                                                          tCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCa                                                                                            >  8:274351/1‑149 (MQ=255)
                                                             aaGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCCCCTTCGGGTGGCCGTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACg                                                                                          >  1:235451/1‑148 (MQ=255)
                                                                             aCCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCg                                                                             <  4:287358/145‑1 (MQ=255)
                                                                             aCCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCg                                                                             >  3:287358/1‑145 (MQ=255)
                                                                              ccGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAAt                                                                        <  3:178910/149‑1 (MQ=255)
                                                                                      gTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCAc                                                                                           >  3:102507/1‑122 (MQ=255)
                                                                                      gTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCAc                                                                                           <  4:102507/122‑1 (MQ=255)
                                                                                          aTGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCt                                                                                                                            >  2:146317/1‑85 (MQ=255)
                                                                                          aTGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCt                                                                                                                            <  1:146317/85‑1 (MQ=255)
                                                                                                  cTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTcgcg                                                      >  5:157170/1‑148 (MQ=255)
                                                                                                   tGTTGCGGCCGTGTAAGTTAGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGACGCAAACCGTTCCAGTGCGGCAAGCAGTTTGGGGTCGGTAATGACCTcgg                                                                >  3:206249/1‑137 (MQ=255)
                                                                                                    gTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAg                                                   >  5:150854/1‑149 (MQ=255)
                                                                                                       gCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGct                                                >  5:243170/1‑149 (MQ=255)
                                                                                                          gCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCTGTAATGCCCTCGGCGGTGTCGCGCAGGCtctc                                             <  6:243170/149‑1 (MQ=255)
                                                                                                            cGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCgg                                           <  5:210420/149‑1 (MQ=255)
                                                                                                                   gTTCGAACACTCTCTAGTTCATCAAGGCCACCTTCGGGTTGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCt                                    <  3:28390/149‑1 (MQ=255)
                                                                                                                       gAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGtt                                 <  6:157170/148‑1 (MQ=255)
                                                                                                                         acacTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGGCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGa                              <  3:278432/149‑1 (MQ=255)
                                                                                                                             tctctAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACAGTACCAGTGCGGCAGGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCCAA‑AGc                         >  4:219651/1‑149 (MQ=255)
                                                                                                                               tctAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTc                       >  4:110118/1‑149 (MQ=255)
                                                                                                                                 tAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCTCTGTTCGAA‑AGCTc                       <  1:21514/147‑1 (MQ=255)
                                                                                                                                   gTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCcgcg                                      <  8:235791/131‑1 (MQ=255)
                                                                                                                                   gTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCcgcg                                      >  7:235791/1‑131 (MQ=255)
                                                                                                                                    ttCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGtt                                 <  8:130038/135‑1 (MQ=255)
                                                                                                                                    ttCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGtt                                 >  7:130038/1‑135 (MQ=255)
                                                                                                                                    ttCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCg                  >  4:54436/1‑149 (MQ=255)
                                                                                                                                     taaaaaaGGCCACCCGAAGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAg                                                   <  1:79693/111‑1 (MQ=255)
                                                                                                                                     taaaaaaGGCCACCCGAAGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAg                                                   >  2:79693/6‑116 (MQ=255)
                                                                                                                                      cATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGc                 <  7:274351/148‑1 (MQ=255)
                                                                                                                                      cATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCa                >  1:121823/1‑149 (MQ=255)
                                                                                                                                       aTTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCa                <  1:186760/148‑1 (MQ=255)
                                                                                                                                       aTTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCAt               <  2:269974/149‑1 (MQ=255)
                                                                                                                                       aTTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGAGCT‑TTCGAACAGCGCGGCCgaga               <  8:233289/149‑4 (MQ=255)
                                                                                                                                        ttAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCGAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCGTg              >  8:300053/1‑149 (MQ=255)
                                                                                                                                        ttAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTc                                >  4:140726/1‑132 (MQ=255)
                                                                                                                                        ttAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTc                                <  3:140726/132‑1 (MQ=255)
                                                                                                                                          aaGGCCACCCGAAGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCATGcc            >  6:67887/1‑149 (MQ=255)
                                                                                                                                           aGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCATGcc            <  6:20697/148‑1 (MQ=255)
                                                                                                                                            ggCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTCGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGATTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCAGTTCGAA‑AGCTCGGCCGCATGCCCg          >  6:158468/1‑149 (MQ=255)
                                                                                                                                                  cTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCATGCCCGCCACGc    >  6:82707/1‑149 (MQ=255)
                                                                                                                                                  cTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGGCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCATGCCCGCCACGc    >  6:262376/1‑149 (MQ=255)
                                                                                                                                                     cGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCATGCCCGCCACGCtt  <  3:110118/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AACAACCAGCTGTTGCGCAAGCTGATTGCTGAAACCGATGCCTGGGAAGTGGTCACTTTCGAAGATGCCAGCACGGCACCGATCTCGTACATGCGCC‑CTGTTGCGGCCGTGTAAGTTCGAACACTCTCTAGTTCATTAAGGCCACCTTCGGGTGGCCTTTTTTATGGCTTGCCCTGGGCGTGGGCCGCCAACCGTTCCAGTGCGGCACGCAGTTTGGGGTCGGTAATGCCCTCGGCGGTGTCGCGCAGGCTCTCGGCCGCGCTGTTCGAA‑AGCTCGGCCGCATGCCCGCCACGCTT  >  NC_002947/1531085‑1531380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑