Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,544,109 T→A 8.2% C305S (TGC→AGC)  mdfA → multidrug MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,544,1090TA 8.2% 136.1 / 3.8 49C305S (TGC→AGC) mdfAmultidrug MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (19/26);  new base A (2/2);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

GTTGAGTACCCTGGAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCTGTACCGCATGACGCTGTTCTCCAGCGAAC  >  NC_002947/1543964‑1544238
                                                                                                                                                 |                                                                                                                                 
gTTGAGTACCCTGGAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGcc                                                                                                                                <  8:2652/149‑1 (MQ=255)
gTTGAGTACCCTGGAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGcc                                                                                                                                >  4:85842/1‑149 (MQ=255)
     gTACCCTGGAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATg                                                                                                                           <  1:298838/149‑1 (MQ=255)
        cccTGGAGTATGGCTTGTGGCAGTTGCCGTGTTTTTGCGGGGTGATACTCGGTAAGCTGATCATCAACCGTATTGCCGATCGCTACCCTCTGCCAGACATGGTGCGTGGCGCGCTCTGGCCATACCTGGATGGCCTGTGCCTGATGGTg                                                                                                                        <  4:187504/149‑1 (MQ=255)
         ccTGGAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGc                                                                                                                       >  5:299404/1‑149 (MQ=255)
           ttgAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCGTGGTGCGTGGCGCGCTCTGGCCATACCGGGCTGGCCTGTGCCTGATGGTGCTg                                                                                                                     >  2:21946/3‑149 (MQ=255)
           tGGAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTg                                                                                                                     >  2:26800/1‑149 (MQ=255)
             gAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGc                                                                                                                   <  7:129254/149‑1 (MQ=255)
                    ccTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTg                                                                                                             <  3:205258/148‑1 (MQ=255)
                              gTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGa                                                                                                  <  4:254424/149‑1 (MQ=255)
                                                gCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGtggtgg                                                                                >  8:89312/1‑149 (MQ=255)
                                                 cTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGc                                                                               <  4:28961/149‑1 (MQ=255)
                                                   gATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGa                                                                                                                 >  1:234498/1‑113 (MQ=255)
                                                   gATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGa                                                                                                                 <  2:234498/113‑1 (MQ=255)
                                                       cTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCAt                                                                         <  8:112419/149‑1 (MQ=255)
                                                             aaCCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCt                                                                   >  4:90557/1‑149 (MQ=255)
                                                              aCCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTg                                                                  >  6:299502/1‑149 (MQ=255)
                                                                cTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTa                                                                >  7:290838/1‑149 (MQ=255)
                                                                 tGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTAc                                                               >  1:277601/1‑149 (MQ=255)
                                                                       tcaACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTg                                                         >  6:301036/1‑149 (MQ=255)
                                                                         aaCCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTgg                                                        <  2:277601/148‑1 (MQ=255)
                                                                           ccGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGt                                                     >  4:200564/1‑149 (MQ=255)
                                                                              tATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTgg                                                  <  3:85842/149‑1 (MQ=255)
                                                                                  gCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGt                                              >  1:270006/1‑149 (MQ=255)
                                                                                   ccGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGc                                                                                                >  8:221551/1‑98 (MQ=255)
                                                                                   ccGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGc                                                                                                <  7:221551/98‑1 (MQ=255)
                                                                                   ccGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTg                                             <  5:299502/149‑1 (MQ=255)
                                                                                     gATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGc                                           <  2:270006/149‑1 (MQ=255)
                                                                                      aTCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGc                                           >  3:83900/1‑148 (MQ=255)
                                                                                         gCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCaa                                        <  4:83900/148‑1 (MQ=255)
                                                                                          cTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATg                                      >  6:44486/1‑149 (MQ=255)
                                                                                          cTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCCGGTACTGGCCGAGCGTGACCAgtg                                                                                      <  1:22332/101‑1 (MQ=255)
                                                                                          cTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCAGGTACTGGCCGAGCGTGACCAgtg                                                                                      >  2:22332/1‑101 (MQ=255)
                                                                                               gaggatcAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTg                                 <  3:276863/143‑1 (MQ=255)
                                                                                               cGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTg                                 <  5:44486/149‑1 (MQ=255)
                                                                                               cGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCAGAGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGc                                           <  3:90547/139‑1 (MQ=255)
                                                                                               cGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCAGAGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGc                                           >  4:94924/1‑139 (MQ=255)
                                                                                               cGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCAGAGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGc                                           <  3:94924/139‑1 (MQ=255)
                                                                                               cGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCAGAGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGc                                           >  4:90547/1‑139 (MQ=255)
                                                                                                 cTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCt                               <  8:290838/149‑1 (MQ=255)
                                                                                                          cTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCTTTACCGCAt                      >  7:289830/1‑149 (MQ=255)
                                                                                                             gTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTgg                                                  <  4:97508/118‑1 (MQ=255)
                                                                                                             gTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCTGTACCGCATg                     <  3:200564/147‑1 (MQ=255)
                                                                                                                 gTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCTGTACCGCATGACGCTg               <  3:90557/149‑1 (MQ=255)
                                                                                                                    gcgcgcTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCTGTACCGCATGACGCTGtt             <  6:299404/148‑1 (MQ=255)
                                                                                                                        gcTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCTGTACCGCATGACGCTGTTCTCCa        <  5:301036/149‑1 (MQ=255)
                                                                                                                              gCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCTGTACCGCATGACGCTGTTCTCCAGCGAAc  <  7:89312/149‑1 (MQ=255)
                                                                                                                               ccATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCt                                                                   <  5:203026/83‑1 (MQ=255)
                                                                                                                               ccATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCt                                                                   >  6:203026/1‑83 (MQ=255)
                                                                                                                                                 |                                                                                                                                 
GTTGAGTACCCTGGAGTATGCCTTGTGGCAGTTGCCGGTTTTTTGCGGGCTGATCCTCGGTAACCTGATCATCAACCGTATTGCCGATCGCTACCCGCTGCCAGCCCTGGTGCGTGGCGCGCTCTGGCCATACCTGGCTGGCCTGTGCCTGATGGTGCTGGCGACCTGGTACTGGCCGAGCGTGACCAGTGTGGTGGCCGGCATGTCGCTGTACGCGCTGGGGTTGGGCGTGGCCAATGCAGTGCTGTACCGCATGACGCTGTTCTCCAGCGAAC  >  NC_002947/1543964‑1544238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑