| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,544,504 | T→G | 15.4% | intergenic (+63/+51) | mdfA → / ← ampG | multidrug MFS transporter/muropeptide permease AmpG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,544,504 | 0 | T | G | 15.4% | 60.8 / 6.4 | 26 | intergenic (+63/+51) | mdfA/ampG | multidrug MFS transporter/muropeptide permease AmpG |
| Reads supporting (aligned to +/- strand): ref base T (11/11); new base G (2/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01 | |||||||||||
TGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAGGCAC > NC_002947/1544365‑1544650 | gggcgggcgCGTTGGCGCGGGGGCCGCTGGGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGCACCTCGATAAACGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGC‑TC‑CTACAAAGCGGCGCGGCCCTGTGTAGGAGc < 6:240938/148‑1 (MQ=21) ggggggggCGTTGGCGCTGTGGCCGGTGGGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGC‑TC‑CTACAAAGCGGCGCGGCCCTGTGTAGGAGCg < 3:65770/146‑1 (MQ=18) gggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGttt < 1:76121/149‑1 (MQ=255) gggCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAACCCCGC‑GC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAATAGGc < 5:222221/148‑1 (MQ=255) gcCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTAGCGGGTAAAACCGC‑TC‑CTAAAACGGGACGCGCCGCGTTGTATGAGCGGGTTTAACCGCGAATAGGCCAGAACCTGCAATAAAAAGCTTCAGTGcc > 1:203844/1‑149 (MQ=255) ggggCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGC‑TC‑CTACACAGGGCCGCGCCGCGTTGTAGGAGCGGGTTTTCCCGCGATGAGGCCCGCACAGGCAATTCAAAGCTTCAGGGCCGGTCAGCCAc > 5:313533/1‑149 (MQ=37) gCTGTAGCTCGATAATCGGTATTCCCGGGGCCGGCCTCTTCGCGGGTAAACCCGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCaa < 1:85548/119‑1 (MQ=255) gCCGGGGCCGGCCTCTGCGCGGGTAAACCCGC‑TC‑CTCCCCAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTc < 2:203844/148‑1 (MQ=255) ccTCTTCGCGGGTAAACCCGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGCGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCt < 5:66042/149‑1 (MQ=255) ctctTCGCGGGTAAACCCGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGcc > 1:11226/1‑127 (MQ=255) ctctTCGCGGGTAAACCCGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGcc < 2:11226/127‑1 (MQ=255) cgcgGGTAAACCCCC‑AC‑CGAAACACGGCCGCGCCGCTTTGTAGGCGCGGGTTTACCCGCGAAGAGGGCAGCCCACGCAATACAAACGGTAAGGGCCGCTCAGCCACCGAGTGGTCGTCCGGCACCCGCGTCCCCCGCCCCGCTTc > 4:298540/1‑145 (MQ=255) cgGGTAAACCCGCTTCGCTACACAGGCCCGCGCCGCTTTGTAGGAGGGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa < 1:108680/149‑1 (MQ=38) cccGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg > 4:169867/1‑147 (MQ=255) cccGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg > 8:227047/1‑147 (MQ=255) cccGC‑TC‑CTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg > 8:20023/1‑147 (MQ=25) ccGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATCGCCCTGCAg > 4:288301/1‑149 (MQ=255) c‑TC‑CTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCCGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc > 5:202748/1‑149 (MQ=21) cTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCg < 3:169867/147‑1 (MQ=255) caccGGGCCGCGCCGCTTTGTAGGCGGGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca < 7:218427/148‑1 (MQ=255) cacaGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCCGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCcatcag > 8:179738/1‑149 (MQ=255) caGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcagga > 8:274000/1‑149 (MQ=255) gggCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggat > 5:71013/1‑148 (MQ=255) ccgcgccgcTTTGTAGGCGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGc < 3:298540/149‑1 (MQ=255) ccgcgccgcTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATCGCCCTGCAGTGGcatcaggaagccg < 3:288301/149‑6 (MQ=38) cTTTGTAGAAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAGGCAc > 1:138836/1‑149 (MQ=255) tttGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggatca < 5:189901/138‑1 (MQ=255) tttGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggatca > 6:189901/1‑138 (MQ=255) | TGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGC‑TC‑CTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAGGCAC > NC_002947/1544365‑1544650 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |